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Sulfhydryl oxidation induces calcium release from fragmented sarcoplasmic reticulum even in the presence of glutathione

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Abstract

Alcian blue and plumbagin induced transient Ca2+ release from fragmented sarcoplasmic reticulum. Dithiothreitol (DTT) and glutathione (GSH) partially blocked Ca2+ release induced by these oxidizing compounds. Pretreatment of alcian blue and plumbagin with DTT or GSH for more than 1 min was required to abolish the ability of the oxidizing compounds to release Ca2+. Mg2+ and ruthenium red completely blocked alcian blue-and plumbagin-induced Ca2+ release. These results suggest that oxidation of sulfhydryls on Ca2+ release channels induces Ca2+ release even in the presence of GSH in situ.

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Authors and Affiliations

  1. Department of Physiology, Nagoya City University Medical School, Mizuho-ku, 467, Nagoya, (Japan)

    M. Koshita, K. Miwa & T. Oba

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  1. M. Koshita
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  2. K. Miwa
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  3. T. Oba
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Koshita, M., Miwa, K. & Oba, T. Sulfhydryl oxidation induces calcium release from fragmented sarcoplasmic reticulum even in the presence of glutathione. Experientia 49, 282–284 (1993). https://doi.org/10.1007/BF01923402

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  • DOI: https://doi.org/10.1007/BF01923402

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