Skip to main content
Log in

ATP assay on cell monolayers as an index of cytotoxicity

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Andersson M, Forsby A, Lewan L (1990) Determination of ATP leakage from cultured cells in toxicity testing: a two step bioluminiscent assay. ATLA 17:188–190

    Google Scholar 

  • Babich H, Shopsis C, Borenfreund E (1986) In vitro Cytotoxicity testing of aquatic pollutants (cadmium, copper, zinc, nickel) using established fish cell lines. Ecotoxicol Environ Saf 11:91–99

    Google Scholar 

  • Becerril C, Hidalgo R, Santamaria A, Sanz F (1989) Determinación de ATP en células V79 y CHO. 1st Meeting Soc. Esp. Mutag. Ambient. Barcelona (Spain)

  • Bols NC, Boliska SA, Dixon DG, Hodson PV, Kaiser KLE (1985) The use of fish cell cultures as an indication of contaminant toxicity to fish. Aquat Toxicol 6:147–155

    Google Scholar 

  • Block JC, Bauda P, Reteuna C (1989) Ecotoxicity testing using aquatic bacteria. In: Boudou A & Ribeyre F (eds) Aquatic Ecotoxicology Fundamental Concepts and Methodologies, vol 2. CRC Press, Boca Raton, p 187

    Google Scholar 

  • Bridges JW, Benford DJ, Hubbard SA (1983) Mechanisms of toxic injury. Ann NY Acad Sci, vol 407, p 42

    Google Scholar 

  • Chapin RE, Gray TJB, Phelps JL, Dutton SL (1988) The effects of mono-(2-ethylhexyl)-phthalate on rat sertoli cell-enriched primary cultures. Toxicol Appl Pharmacol 92:467–479

    Google Scholar 

  • De Luca MA, McElroy WD (1981) Bioluminiscence and Chemiluminiscence. Academic Press, New York, p 122

    Google Scholar 

  • Galli CL, Viviani B, Casartelli G, Marinovich M (1991) ATP and protein synthesis assays to evaluate the toxicities of preservatives in vitro. ATLA 19:60–67

    Google Scholar 

  • Gille JJP, Berkel CGM, Mullaart E, Vijg J, Joenje H (1989) Effects of lethal exposure to hyperoxia and to hydrogen peroxide on NAD(H) and ATP pools in Chinese hamster ovary cells. Mutat Res 214:89–96

    Google Scholar 

  • Kemp RB, Cross DM, Meredith RWJ (1986) Adenosine triphosphate as an indicator of cellular toxicity in vitro. Fd Chem Toxic 24:465–466

    Google Scholar 

  • Kemp RB, Meredith RWJ, Gamble SH (1985) Toxicity of commercial products on cells in suspension culture: A possible screen for the draize eye irritation test. Fd Chem Toxic 23:267–270

    Google Scholar 

  • Knox P, Uphill PF, Fry JR, Benford J, Balls M (1986) The frame multicentre project on in vitro cytotoxicology. Fd Chem Toxic 24:457–463

    Google Scholar 

  • Köszegi T, Kellermayer M, Kövecs F, Jobst K (1988) Bioluminescent monitoring of ATP release from human red blood cells (treated with nonionic detergent). J Clin Chem Clin Biochem 26:599–604

    Google Scholar 

  • Nungester WJ, Paradise LJ, Adair JA (1969) Loss of cellular ATP as a measure of cytolytic activity of antiserum Proc Soc Exp Biol Med 132:582

    Google Scholar 

  • Trevors JT (1986) A basic program for estimating LD50 values using the IBM-PC. Bull Environ Contain Toxicol 37:18–26

    Google Scholar 

  • Vernetti LA, MacDonald JR, Wolfgang GHI, Dominick MA, Pegg DG (1993) ATP depletion is associated with cytotoxicity of novel lipid regulator in guinea pig adrenocortica cells. Toxicol Appl Pharmacol 118:30–38

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Present address: Sanidad Animal, INIA, E-28130 Valdeolmos, Madrid, Spain

Rights and permissions

Reprints and permissions

About this article

Cite this article

Castaño, A., Tarazona, J.V. ATP assay on cell monolayers as an index of cytotoxicity. Bull. Environ. Contam. Toxicol. 53, 309–316 (1994). https://doi.org/10.1007/BF00192050

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00192050

Keywords

Navigation