Abstract
Nitrogenase activity, nitrogenase synthesis, and the growth of four species of Azospirillum were examined at 30°C and 42°C. Azospirillum brasilense Sp 7 did not synthesize nitrogenase at 42°C nor was the enzyme stable at this temperature. In A. lipoferum 708 and A. brasilense 12S the nitrogenase activity was stable at 42°C but the synthesis of nitrogenase was reduced. In A. brasilense Sp 9 the nitrogenase activity was stable and showed the highest C2H2 reduction activity at 42°C.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brooks SJ, Collins JJ, Brill WJ (1984) Repression of nitrogen fixation in Klebsiella pneumoniae at high temperature. J Bacteriol 157:460–464
Bulow JWF, Döbereiner J (1975) Potential for nitrogen fixation in maize genotypes in Brazil. Proc Natl Acad Sci USA 72:2389–2393
Chaudhary K, Yadav KS, Tauro P (1992) Nitrogen fixation by Azospirillum brasilense: temperature effect. Indian J Microbiol 32: 41–44
Collins JJ, Brill WJ (1985) Control of Klebsiella pneumoniae nif mRNA synthesis. J Bacteriol 162:1186–1190
Hennecke H, Shanmugam KT (1979) Temperature control of nitrogen fixation in Klebsiella pneumoniae. Arch Microbiol 123:259–265
Kapulnik Y, Kigel J, Okon Y, Nur I, Henis Y (1981) Effect of Azospirillum inoculum on some growth parameters and N-content of wheat, sorghum and panicum. Plant Soil 61:65–70
Kundu BS (1992) Physiological studies of typical strain of A. brasilense 12S from sorghum roots. Indian J Exp Biol 30: 1188–1192
Okon Y, Albrecht SL, Burris RH (1976) Factors affecting growth and nitrogen fixation of Spirillum lipoferum. J Bacteriol 127:1248–1254
Purushothaman D, Vigila K (1989) Accumulation of proline and poly-B-hydroxybutyrate in high temperature resistant strains of Azospirillum. Curr Sci 58:1047–1048
Raju PN, Evans HJ, Seidler RJ (1972) An asymbiotic nitrogen-fixing bacterium from the root environment of corn. Proc Natl Acad Sci USA 69:3474–3478
Wani SP, Chandrapalaiah S, Dart PJ (1985) Response of pearlmillet cultivars to inoculation with nitrogen fixing bacteria. Exp Agric 21:175–182
Wani SP, Chandrapalaiah S, Zambra MA, Lee KK (1988) Association between N2-fixing bacteria and pearlmillet. Plant Soil 110: 289–302
Zhu JB, Li ZG, Wang LW, Shen SS, Shen SC (1986) Temperature sensitivity of a nif A like gene in Enterobacter cloacae. J Bacteriol 166:357–359
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Aggarwal, P., Chaudhary, K. Biological nitrogen fixation at elevated temperature in different Azospirillum species and strains. Biol Fertil Soils 20, 260–262 (1995). https://doi.org/10.1007/BF00336087
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00336087