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Comparison of various BCG preparations in the EORTC — ICIG experimental screening for systemic immunity adjuvants applicable to cancer immunotherapy

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Summary

Nine lyophilized, five frozen, and one fresh BCG preparation from various sources were tested for their activity as adjuvants of systemic immunity in mice. Using three tests, the IgM response to sheep red blood cells, immunoprophylaxis of L1210 leukemia, and immunoprophylaxis of Lewis tumor, results consistent with both immunostimulation and immunodepression were observed, varying as a function of the BCG strain, its storage conditions, and the assay used. Only fresh Pasteur strain BCG was effective in all three tests; its activity was modified by lyophilization and by freezing. Possible reasons for the superiority of fresh Pasteur BCG as an adjuvant are discussed.

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Authors and Affiliations

  1. Institut de Cancérologie et d'Immunogénétique (INSERM), Hôpital Paul Brousse, 14-16 avenue Paul-Vaillant-Couturier, F-94800, Villejuif, France

    O. Halle-Pannenko, Ch. Bourut & M. Kamel

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  1. O. Halle-Pannenko
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  2. Ch. Bourut
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  3. M. Kamel
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European Organization for Research and Treatment of Cancer

Institut de Cancérologie et d'Immunogénétique

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Halle-Pannenko, O., Bourut, C. & Kamel, M. Comparison of various BCG preparations in the EORTC — ICIG experimental screening for systemic immunity adjuvants applicable to cancer immunotherapy. Cancer Immunol Immunother 1, 17–23 (1976). https://doi.org/10.1007/BF00205289

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