Skip to main content
SpringerLink
Log in

Characterization of crotonate grown Clostridium kluyveri by its assimilatory metabolism

  • Published:
Archiv für Mikrobiologie Aims and scope Submit manuscript

Summary

Considerable behavioral differences were observed during growth of Clostridium kluyveri on ethanol-acetate and on crotonate media. The identity of the crotonate grown Clostridium with the ethanol grown Clostridium kluyveri was therefore established by three characteristic biosynthetic routes: 1. ribose is synthesized from CO2 and acetate via pyruvate, triose phosphate and a non-oxidative pentose phosphate pathway, 2. reduced one-carbon units are formed predominantly from CO2 and not from serine as usual, and 3. glutamate biogenesis follows an atypical stereochemical course.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barker, H. A., and S. M. Taha: Clostridium kluyveri, an organism concerned in the formation of caproic acid from ethyl alcohol. J. Bact. 43, 347–363 (1942).

    Google Scholar 

  • Bergmeyer, H. U., G. Holz, H. Klotsch, u. G. Lang: Phosphotransacetylase aus Clostridium kluyveri. Biochem. Z. 338, 114–121 (1963).

    PubMed  Google Scholar 

  • Decker, K., Chr. Barth u. H. Metz: Die Kohlenhydratsynthese in Clostridium kluyveri. II. Enzymatische Studien. Biochem. Z. 345, 472–492 (1966a).

    Google Scholar 

  • — u. K. Jungermann: Die Kohlenhydratsynthese in Clostridium kluyveri. I. Isotopen-Versuche zur Biosynthese der Ribose. Biochem. Z. 345, 461–471 (1966b).

    Google Scholar 

  • —, and S. V. Hunt: Net CO2 fixation into the S-methyl group of methionine and into the positions 2 and 8 of the purines in Clostridium kluyveri. Biochim. biophys. Acta (Amst.) 141, 202–204 (1967).

    Google Scholar 

  • Gottschalk, G., and H. A. Barker: Synthesis of Glutamate and Citrate by Clostridium kluyveri. A new type of citrate synthase. Biochemistry 5, 1125–1133 (1966)

    PubMed  Google Scholar 

  • ——: Presence and stereospecificity of citrate synthase in anaerobic bacteria. Biochemistry 6, 1027–1034 (1967).

    PubMed  Google Scholar 

  • Jungermann, K., R. K. Thauer, and K. Decker: The synthesis of one-carbon units from CO2 in Clostridium kluyveri. Europ. J. Biochem. 3, 351–359 (1968a).

    PubMed  Google Scholar 

  • —, and K. Decker: Confirmation of unusual stereochemistry of glutamate biosynthesis in Clostridium kluyveri. FEBS Letters 1, 74–76 (1968b).

    Article  PubMed  Google Scholar 

  • Thauer, R. K., K. Jungermann, and K. Decker: A quantitative isotope method for regulation studies of aromatic amino acid synthesis under growth conditions. Europ. J. Biochem. 1, 482–486 (1967).

    PubMed  Google Scholar 

  • —, and K. Decker: The energy metabolism of Clostridium kluyveri. Europ. J. Biochem. 4, 173–180 (1968).

    PubMed  Google Scholar 

  • Tomlinson, N.: Carbon dioxide and acetate utilization by Clostridium kluyveri. III. A new path of glutamic acid synthesis. J. biol. Chem. 209, 605–609 (1954).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biochemisches Institut der Universität Freiburg, Freiburg, Germany

    Rudolf K. Thauer, Kurt Jungermann, Joseph Wenning & Karl Decker

Authors
  1. Rudolf K. Thauer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kurt Jungermann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Joseph Wenning
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Karl Decker
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This paper contains parts of the doctoral thesis of J. W., Medizinische Fakultät, Universität Freiburg, 1968.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thauer, R.K., Jungermann, K., Wenning, J. et al. Characterization of crotonate grown Clostridium kluyveri by its assimilatory metabolism. Archiv. Mikrobiol. 64, 125–129 (1968). https://doi.org/10.1007/BF00406971

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00406971

Keywords

Search

Navigation