Abstract
The somatic cell hybrid C121, with chromosome 7 as its sole human component, arose when mouse macrophages were immortalized by fusion with SV40-transformed human fibroblasts. The transforming SV40 genomes are integrated at 7q31-7q35. We show that hybrids with a reduced chromosome 7 component, but which retain markers linked to the cystic fibrosis locus, can be generated by direct in vivo tumor selection or following chromosome-mediated gene transfer and SV40-mediated cellular transformation. Our methods for chromosome fragmentation and fine-structure mapping can now be applied to the substantial number of SV40-transformed human cell lines, with independent chromosomal integration sites, already available. Our results also suggest that expression of human epidermal growth factor receptor augments the tumorigenic potential of the SV40-transformed C121 hybrid.
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Porteous, D.J., Dorin, J.R., Wilkinson, M.M. et al. SV40-mediated tumor selection and chromosome transfer to enrich for cystic fibrosis region. Somat Cell Mol Genet 16, 29–38 (1990). https://doi.org/10.1007/BF01650477
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DOI: https://doi.org/10.1007/BF01650477