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Analysis of WAGR deletions and related translocations with gene-specific DNA probes, using FACS-selected cell hybrids

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Somatic Cell and Molecular Genetics

Abstract

We used the fluorescence-activated cell sorter (FACS) to select a series of somatic cell hybrids with deleted or translocated chromosome 11 segregated from its normal homolog. Analysis of these cell hybrids with gene-specific probes and for cell-surface marker expression has allowed us to order the markers and define a smallest region of overlap (SRO) for deletions associated with the WAGR (Wilms' tumor, aniridia, genitourinary abnormalities, and mental retardation) region of chromosome 11. Two translocation breakpoints in 11 p13 (one associated with familial aniridia and one with a sporadic case of congenital renal dysfunction resulting from urethral and ureteral atresia) map within this SRO.

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Authors and Affiliations

  1. MRC Clinical and Population Cytogenetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK

    Anne Seawright, Judy M. Fletcher, Judy A. Fantes, Harris Morrison & David J. Porteous

  2. Laboratory of Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, 27709, Research Triangle Park, North Carolina

    Steven S. -L. Li, Nicholas D. Hastie & Veronica Van Heyningen

Authors
  1. Anne Seawright
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  2. Judy M. Fletcher
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  3. Judy A. Fantes
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  4. Harris Morrison
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  5. David J. Porteous
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  6. Steven S. -L. Li
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  7. Nicholas D. Hastie
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  8. Veronica Van Heyningen
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Seawright, A., Fletcher, J.M., Fantes, J.A. et al. Analysis of WAGR deletions and related translocations with gene-specific DNA probes, using FACS-selected cell hybrids. Somat Cell Mol Genet 14, 21–30 (1988). https://doi.org/10.1007/BF01535046

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  • DOI: https://doi.org/10.1007/BF01535046

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