Abstract
The transport characteristics of immunoisolation membranes can have a critical effect on the design of hybrid artificial organs and cell therapies. However, it has been difficult to quantitatively evaluate the desired transport properties of different hollow fiber membranes due to bulk mass transfer limitations in the fiber lumen and annular space. An attractive alternative to existing methodologies is to use the rate of solute removal or “washout” from the annular space during constant flow perfusion through the fiber lumen. Experimental washout curves were obtained for glucose and a 10 kD dextran in two different hollow fiber devices. Data were analyzed using a theoretical model which accounts for convective and diffusive transport in the lumen, membrane, and annular space. The model was in good agreement with the experimental results and provided an accurate measure of the effective membrane diffusion coefficient for both small and large solutes. This approach should prove useful in theoretical analyses of solute transport and performance of hollow fiber artificial organs. © 1998 Biomedical Engineering Society.
PAC98: 8722Fy, 8790+y
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REFERENCES
Aebischer, P., E. Buchser, J. Joseph, J. Favre, N. de Tribolet, M. Lysaght, S. Rudnick, and M. Goddard. Transplantation in humans of encapsulated xenogeneic cells without immunosuppression: A preliminary report. Transplantation58:1275- 1277, 1994.
Brotherton, J. D., and P. C. Chau. Modeling of axial-flow hollow fiber cell culture bioreactors. Biotechnol. Prog.12:575-590, 1996.
Catapano, G. Mass transfer limitations to the performance of membrane bioartificial liver support devices. Int. J. Artif. Organs19:18-35, 1996.
Chick, W. L., A. A. Like, and V. Lauris. Beta cell culture on synthetic capillaries: An artificial endocrine pancreas. Science187:847-849, 1975.
Colton, C. K., and E. S. Avgoustiniatos. Bioengineering in development of the hybrid artificial pancreas. J. Biomech. Eng.113:152-170, 1991.
Colton, C. K., and E. G. Lowrie. Hemodialysis: Physical principles and technical considerations. In: The Kidney, edited by B. M. Brenner and F. C. Rector. Philadelphia: Saunders, 1981, pp. 2425-2489.
Deen, W. M. Hindered transport of large molecules in liquid-filled pores. AIChE. J.33:1409-1425, 1987.
Dionne, K. E., B. M. Cain, R. H. Li, E. J. Doherty, M. J. Lysaght, D. H. Rein, and F. T. Gentile. Transport characterization of membranes for immunoisolation. Biomaterials17:257-271, 1996.
Giorgio, T. D., A. D. Moscioni, J. Rozga, and A. A. Demetriou. Mass transfer in a hollow fiber device used as a bioartificial liver. ASAIO J.39:886-892, 1993.
Granath, K. A. Solution properties of branched dextrans. J. Colloid Sci.13:308-322, 1958.
Jesser, C., L. Kessler, A. Lambert, A. Belcourt, and M. Pinget. Pancreatic islet macroencapsulation: A new device for the evaluation of artificial membrane. Artif. Organs20:997- 1007, 1996.
Kelsey, L. J. Fluid flow and mass transfer in hollow fiber membrane devices. Ph.D. Thesis, University of Delaware, Newark, DE, 1992.
Kelsey, L. J., M. R. Pillarella, and A. L. Zydney. Theoretical analysis of convective flow profiles in a hollow-fiber membrane bioreactor. Chem. Eng. Sci.45:3211-3220, 1990.
Kessler, L., M. Pinget, M. Aprahamian, D. Poinsot, M. Keipes, and C. Damgé. Diffusion properties of an artificial membrane used for Langerhans islets encapsulation: Interest of an in vitrotest. Transplant. Proc.24:953-954, 1992.
Knazek, R. A., P. M. Gullino, P. O. Kohler, and R. L. Dedrick. Cell culture on artificial capillaries: An approach to tissue growth in vitro. Science178:65-67, 1972.
Langsdorf, L. J., and A. L. Zydney. Diffusive and convective solute transport through hemodialysis membranes: A hydrodynamic analysis. J. Biomed. Mater. Res.28:573-582, 1994.
Mochizuki, S., and A. L. Zydney. Dextran transport through asymmetric ultrafiltration membranes: Comparison with hydrodynamic models. J. Membrane Sci.68:21-41, 1992.
Pillarella, M. R., and A. L. Zydney. Theoretical analysis of the effect of convective flow on solute transport and insulin release in a hollow-fiber bioartificial pancreas. J. Biomech. Eng.112:220-228, 1990.
Ramírez, C. A., M. López, and C. L. Stephens. In vitroperfusion of hybrid artificial pancreas devices at low flow rates. ASAIO J.38:M443-M449, 1992.
Scharp, D. W., C. J. Swanson, B. J. Olack, P. P. Latta, O. D. Hegre, E. J. Doherty, F. T. Gentile, K. S. Flavin, M. F. Ansara, and P. E. Lacy. Protection of encapsulated human islets implanted without immunosuppression in patients with Type I or Type II diabetes and in nondiabetic control subjects. Diabetes43:1167-1170, 1994.
Sullivan, S. J., T. Maki, K. M. Borland, M. D. Mahoney, B. A. Solomon, T. E. Muller, A. P. Monaco, and W. L. Chick. Biohybrid artificial pancreas: Long-term implantation studies in diabetic, pancreatectomized dogs. Science252:718-721, 1991.
Ward, R. S., K. A. White, C. A. Wolcott, A. Y. Wang, R. W. Kuhn, J. E. Taylor, and J. K. John. Development of a hybrid artificial pancreas with a dense polyurethane membrane. ASAIO J.39:M261-M267, 1993.
Zekorn, T., U. Siebers, L. Filip, K. Mauer, U. Schmitt, R. G. Bretzel, and K. Federlin. Bioartificial pancreas: The use of different hollow fibers as a diffusion chamber. Transplant. Proc.21:2748-2750, 1989.
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Boyd, R.F., López, M., Stephens, C.L. et al. Solute Washout Experiments for Characterizing Mass Transport in Hollow Fiber Immunoisolation Membranes. Annals of Biomedical Engineering 26, 618–626 (1998). https://doi.org/10.1114/1.102
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DOI: https://doi.org/10.1114/1.102