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Changes in Plasma and Colonic Mucosa Fatty Acid Profiles in Rats with Ulcerative Colitis Induced by Trinitrobenzene Sulfonic Acid

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Abstract

Polyunsaturated fatty acids have a key role inthe pathogenesis of inflammatory bowel disease sincesome of the arachidonic acid-derived eicosanoids havebeen found to be increased in inflamed intestinal mucosa in the acute phase of human disease. Theaim of this study was to prospectively assess plasma andcolon mucosa fatty acid patterns in rats withexperimental ulcerative colitis. Twenty rats weretreated with trinitrobenzene sulfonic acid and 20 withNaCl; two groups were killed after one week and twoafter two weeks to evaluate colon damage. Plasma wasobtained by aortic puncture and colonic mucosa was scraped off and the fatty acid pattern wasdetermined by gas-liquid chromatography. Total,saturated, and monounsaturated plasma fatty acids weresignificantly higher in both periods of ulcerativecolitis as compared to controls. Plasma n-6 fatty acidswere increased after treatment, but no significantchanges were observed concerning to n-3 fatty acids.With regard to colon mucosa, saturated andmonounsaturated fatty acids did not change because of thedisease; however, n-6 fatty acids decreased in the firstweek and increased in the second week and n-3 fattyacids were increased. Changes on the fatty aciddistribution in plasma did not parallel to those of colonicmucosa except for 22:6(n-3). We have also found thatexperimental ulcerative colitis induced bytrinitrobenzene sulfonic acid reproduces many of thefeatures related to changes in plasma and colon mucosafatty acids observed in the human disease.

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Authors
  1. N. Nieto
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  2. M. D. Giron
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  3. M. D. Suarez
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  4. A. Gil
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Nieto, N., Giron, M.D., Suarez, M.D. et al. Changes in Plasma and Colonic Mucosa Fatty Acid Profiles in Rats with Ulcerative Colitis Induced by Trinitrobenzene Sulfonic Acid. Dig Dis Sci 43, 2688–2695 (1998). https://doi.org/10.1023/A:1026607428716

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  • DOI: https://doi.org/10.1023/A:1026607428716

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