Abstract
A cDNA clone GmPM4 which encodes mRNA species in mature or dry soybean seeds was characterized. DNA sequence analysis shows that the deduced polypeptides have a molecular mass of 68 kDa. GmPM4 proteins have a relatively high amino acid sequence homology with a major biotinylated protein isolated from pea seeds, SBP65, but both of these proteins differ markedly from that of presently known biotin enzymes. The accumulation of GmPM4 mRNA is detectable in the leaf primodium and the vascular tissues of the hypocotyl-radicle axis of mature seeds, and the GmPM4 proteins are present at high levels in dry and mature soybean seeds, but not in fresh immature seeds. It degrades rapidly at the early stage of seed germination. These proteins are boiling-soluble and biotinylated when they are present endogenously in soybean seeds; however, the same recombinant protein expressed in Escherichia coli is boiling-soluble, but it is not biotinylated.
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Blackman SA, Wettlaufer SH, Obendorf RL, Leopold AC: Maturation proteins associated with desiccation tolerance in soybean. Plant Physiol 96: 868–874 (1991).
Bewley JD, Black M: Physiology and Biochemistry of Seeds, vol. 1. Development, Germination and Growth, p. 233. Springer-Verlag, Berlin/Heidelberg/New York (1983).
Chandler CS, Ballard FJ: Regulation of the breakdown rates of biotin-containing proteins in Swiss 3T3-L1 cells. Biochem J 251: 749–755 (1988).
Charles DJ, Cherry JH: Purification and characterization of acetyl-CoA carboxylase from developing soybean seeds. Phytochemistry 25: 1067–1071 (1986).
Chen Z, Hsing YC, Lee P, Chow T: Nucleotide sequences of a soybean cDNA encoding an 18 kilodalton late embryogenesis abundant protein. Plant Physiol 99: 773–774 (1992).
Cronan JE Jr: The E. coli operon: transcriptional repression by an essential protein modification enzyme. Cell 58: 427–429 (1989).
Devereux J, Haeberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395 (1984).
Dure L III: Structural motifs in Lea proteins. In: Close TJ, Bray EA (eds) Plant Responses to Cellular Dehydration during Environmental Stress. Current Topics in Plant Physiology, vol. 10, pp. 91–103. American Society of Plant Physiologists Series (1993).
Dure L III, Crouch M, Harada J, Ho T-hD, Mundy J, Quatrano RS, Thomas T, Sung ZR: Common amino acid sequence domains among the LEA proteins of higher plants. Plant Mol Biol 12: 475–486 (1989).
Duval M, DeRose RT, Job D, Faucher D, Douce R, Job D: The major biotinyl protein from Pisum sativum seeds covalently binds biotin at a novel site. Plant Mol Biol 26: 265–273 (1994).
Duval M, Job C, Alban C, Douce R, Job D: Developmental patterns of free and protein-bound biotin during maturation and germination of seeds of Pisum sativum: characterization of a novel seed-specific biotinylated protein. Biochem J 299: 141–150 (1994).
Duval M, Job C, Alban C, Sparace S, Douce R, Job D: Synthesis and degradation of a novel biotinyl protein in developing and germinating pea seeds. C R Acad Sci (Paris), Ser III 316: 1463–1470 (1993).
Duval M, Loiseau J, Deuaye L, Pepin R, Ledeunff Y, Wang T, Job D: SBP65, a seed-specific biotinylated protein, behaves as a LEA protein in developing pea embryos. C R Acad Sci (Paris) Ser III 319: 585–594 (1996).
Duval M, Pepin R, Job C, Derpierre C, Douce R, Job D: Ultra-structural localization of the major biotinylated protein from Pisum sativum seeds. J Exp Bot 46: 1783–1786 (1995).
Fall RR: Analysis of microbial biotin proteins. Meth Enzymol 67: 390–398 (1979).
Franca Neto JB, Shatters RG, West SH: Developmental pattern of biotinylated proteins during embryogenesis and maturation of soybean seed. Seed Sci Res 7: 377–384 (1997).
Furukawa K, Tagaya M, Masayori I, Preiss J, Fukui T: Identification of Lysine 15 at the active site in Escherichia coli glycogen synthase. J Biol Chem 265: 2086–2090 (1990).
Galau GA, Hughes DW, Dure L: Abscisic acid induction of cloned cotton late embryogenesis-abundant (Lea) mRNAs. Plant Mol Biol 7: 155–170 (1986).
Hsing YC, Chen Z, Chow T: Nucleotide sequences of a soybean complementary DNA encoding a 50-kilodalton late embryogenesis abundant protein. Plant Physiol 99: 354–355 (1992).
Hsing YC, Chen Z, Shih M, Hsieh J, Chow T: Unusual sequences of group 3 LEA mRNA inducible by maturation or drying in soybean seeds. Plant Mol Biol 29: 863–868 (1995).
Hsing YC, Hsieh K, Huang Y, Hsieh J: Premature drying and germination in wild soybean seeds. Taiwania 40: 73–81 (1995).
Hsing YC, Lee P, Chen Z, Chow T: A soybean seed cDNA (X63565) encoding a late embryogenesis abundant protein. Plant Physiol 109: 1125 (1995).
Hsing YC, Rinne RW, Hepburn AG, Zielinski RE: Expression of maturation-specific genes in soybean seeds. Crop Sci 30: 1343–1350 (1990).
Hsing YC, Wu S: Cloning and characterization of cDNA clones encoding soybean seed maturation polypeptides. Bot Bull Acad Sin 33: 191–199 (1992).
Hughes DW, Galau GA: Developmental and environmental induction of Lea and LeaA mRNAs and the post-abscission program during embryo culture. Plant Cell 3: 605–618 (1991).
Jackson DP: In situ hybridization in plants. In: Bowles DJ, Gurr SJ, McPhereson M (eds) Molecular Plant Pathology: A Practical Approach, pp. 163–174. Oxford University Press, London (1992).
Knowles JR: The mechanism of biotin dependent enzymes. Annu Rev Biochem 58: 195–221 (1989).
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685 (1970).
Puupponen-pimia R, Saloheimo M, Vasara T, Ra R, Gaugecz J, Kurten U, Knowles JKC, Keranen S, Kauppinen V: Characterization of a birch (Betula pendula Roth.) embryogenic gene, BP8. Plant Mol Biol 23: 423–428 (1993).
Robinson BH, Oei J, Saunders M, Grvel R: 3H-Biotin-labeled proteins in cultured human skin fibroblasts from patients with pyruvate carboxylase deficiency. J Biol Chem 258: 6660–6664 (1983).
Rosenberg LA, Rinne RW: Moisture loss as a prerequisite for seedling growth in soybean seeds (Glycine max (L.) Merr.). J Exp Bot 37: 1663–1674 (1986).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain termination inhibitors. Proc Natl Acad Sci USA 64: 5463–5467 (1977).
Schneider T, Dinkins R, Robinson K, Shellhammer J, Meinke DW: An embryo-lethal mutant of Arabidopsis thaliana is a biotin auxotroph. Devel Biol 131: 161–167 (1989).
Shatters RG, Boo SP, Franca Neto JB, West SH: Identification of biotinylated proteins in soybean (Glycine max L.) seeds and their characterization during germination and seedling growth. Seed Sci Res 7: 373–376 (1997).
Shellhammer J, Meinke D: Arrested embryos from the bio1 auxotroph of Arabidopsis thaliana contain reduced levels of biotin. Plant Physiol 93: 1162–1167 (1990).
Skriver K, Mundy J: Gene expression in response to abscisic acid and osmotic stress. Plant Cell 2: 503–512 (1990).
Song J, Wurtele ES, Nikolau BJ: Molecular cloning and characterization of the cDNA coding for the biotin-containing subunit of 3-methylcrotonoyl-CoA carboxylase: Identification of the biotin carboxylase and biotin-carrier domains. Proc Natl Acad Sci USA 91: 5779–5783 (1994).
Wurtele ES, Nikolau BJ: Plants contain multiple biotin enzymes: discovery of 3-methylcrotonoyl-CoA carboxylase, propionyl-CoA carboxylase and pyruvate carboxylase in the plant kingdom. Arch Biochem Biophys 278: 179–186 (1990).
Yoneya T, Tagaya M, Kishi F, Nagazawa A, Fukui T: Site-directed mutagenesis of Gly-15 and Gly-20 in the glycine-rich region of adenylate kinase. J Biochem 105: 158–160 (1989).
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Hsing, Yi.C., Tsou, Ch., Hsu, Tf. et al. Tissue- and stage-specific expression of a soybean (Glycine max L.) seed-maturation, biotinylated protein. Plant Mol Biol 38, 481–490 (1998). https://doi.org/10.1023/A:1006079926339
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DOI: https://doi.org/10.1023/A:1006079926339