Skip to main content
SpringerLink
Log in

Purification of anhydrotetracycline oxygenase fromStreptomyces rimosus using fast protein liquid chromatography

  • Published:
Biotechnology Techniques

Summary

Anhydrotetracycline oxygenase was purified from cleared cell lysates ofStreptomyces rimosus, using Fast Protein Liquid Chromatography. The preparation was subjected to preparative gel electrophoresis, yielding a single major species which was sufficiently pure to allow direct N-terminal amino acid sequence determination.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Behal, V., Hostalek, Z and Vanek, Z (1979) Biotechnol. Lett. 1, 177–182.

    Google Scholar 

  2. Binnie, C., Warren, M. and Butler, M.J. (1989) J. Bact. 171, 887–895.

    Google Scholar 

  3. Butler, M.J., Friend, E.J., Hunter, I.S., Sugden, D.A. and Warren, M. (1989) Molec. Gen. Genet. 215, 231–238.

    Google Scholar 

  4. Rhodes, P.M., Winskill, N., Friend, E.J. and Warren M (1981) J. Gen Microbiol. 124, 329–338.

    Google Scholar 

  5. Vancurova, I., Volc, J., Flieger, M., Neuzil, J., Novotna, J., Vlach, J. and Behal, V. (1988) Biochem. J. 253, 263–267.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. International Process Development, Pfizer Limited, CT13 9NJ, Sandwich, Kent, UK

    Michael J. Butler

  2. Analytical Laboratory, Pfizer Limited, CT13 9NJ, Sandwich, Kent, UK

    Brian N Gedge

Authors
  1. Michael J. Butler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Brian N Gedge
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Butler, M.J., Gedge, B.N. Purification of anhydrotetracycline oxygenase fromStreptomyces rimosus using fast protein liquid chromatography. Biotechnol Tech 3, 235–238 (1989). https://doi.org/10.1007/BF01876055

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01876055

Keywords

Search

Navigation