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Apoptosis, cell proliferation and in vivo biological behaviour of primary and metastatic tumour cells of an AKR lymphoma variant

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Abstract

The possibility that apoptosis and/or cell proliferation have a role in tumour progression in a murine T cell lymphoma was tested. The model consisted of the comparison of primary (PT) and metastatic tumour (MT) cells. The PT cells, but not the MT cells displayed a very pronounced tendency for spontaneous apoptosis. Proliferative capacity of MT cells was lower than that of PT cells, suggesting that it does not contribute to the metastatic phenotype in this system. Release from apoptosis does however, probably, play a role in the aggressiveness of the lymphoma.

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References

  1. Hall PA, Levison DA. Review: Assessment of cell proliferation in histological material. J Clin Pathol 1990; 17: 1122–1125.

    Google Scholar 

  2. Brandt L, Johnson A, Olsson H, Akerman M. Mitotic activity and survival in advanced non-Hodgkin's lymphoma of unfavorable histology. Eur J Cancer 1990; 26: 227–230.

    Google Scholar 

  3. Macartney JC, Camplejohn RS, Morris R, Hollowood K, Clarke D, Timothy A. DNA flow cytometry of follicular non-Hodgkin's lymphoma. J Clin Pathol 1991; 44: 215–218.

    Google Scholar 

  4. Cotran RS, Kumar V, Robbins SL. Pathologic Basis of Disease, 5th Edition. Philadelphia, PA (USA): WB Saunders Company, 1994: 245–250.

    Google Scholar 

  5. Hockenbery DM. The bcl-2 oncogene and apoptosis. Seminars Immunol 1992; 4: 413–420.

    Google Scholar 

  6. Leoncini L, Del Vecchio MT, Megha T, et al. Correlation between apoptotic and proliferative indices in malignant non-Hodgkin's lymphomas. Am J Pathol 1993; 142: 755–763.

    Google Scholar 

  7. Lotem J, Cragoe EJ Jr, Sachs L. Rescue from programmed cell death in leukemic and normal myeloid cells. Blood 1991; 78: 953.

    Google Scholar 

  8. Collins MKL, Rivas AL. The control of apoptosis in mammalian cells. TIBS 1993; 18: 307–309.

    Google Scholar 

  9. Knight CRL, Rees RC, Griffin M. Apoptosis: a potential role for cytosolic transglutaminase and its importance in tumor progression. Biochim Biophys Acta 1991; 1096: 312–318.

    Google Scholar 

  10. Neiman PE, Thomas SJ, Loring G. Induction of apoptosis during normal and neoplastic B-cell development in the bursa of Fabricius. Proc Natl Acad Sci 1991; 88: 5857–5861.

    Google Scholar 

  11. Kerr JF, Searle J. A model of cell loss in malignant neoplasms. J Pathol 1972; 106: 41.

    Google Scholar 

  12. Kypriano UN, English HF, Davidson NE, Isaacs JT. Programmed cell death during regression of the MCE-7 human breast cancer following estrogen ablation. Cancer Res 1991; 51: 162–166.

    Google Scholar 

  13. Lotem J, Sachs L. Hematopoietic cells from mice deficient in wild-type p53 are more resistant to induction of apoptosis by some agents. Blood 1993; 82: 1092–1096.

    Google Scholar 

  14. Hickman JA, Potten CS, Merritt AJ, Fisher TC. Apoptosis and cancer chemotherapy. Phil Trans R Soc Lond B 1994; 345: 319–325.

    Google Scholar 

  15. Chin KV, Ueda K, Pastan I, Gottesman MM. Modulation of activity of the promotor of the human mdr1 gene by ras and p53. Science 1992; 255: 459–462.

    Google Scholar 

  16. Liu Y, Mullbacker A, Waring P. Natural killer cells and cytotoxic T cells induce DNA fragmentation both in human and murine target cells in vitro. Scand J Immunol 1989; 30: 31–37.

    Google Scholar 

  17. Martz E, Howell DM. CTL: virus control cells first and cytolytic cells second? DNA fragmentation, apoptosis and the prelytic halt hypothesis. Immunol Today 1989; 10: 79–86.

    Google Scholar 

  18. Knight CRL, Rees RC, Platts A, Johnson T, Griffin M. Interleukin-2-activated human effector lymphocytes mediate cytotoxicity by inducing apoptosis in human leukaemia and solid tumor target cells. Immunology 1993; 79: 535–541.

    Google Scholar 

  19. Korsmeyer SJ. bcl-2 initiates a new category of oncogenes: regulators of cell death. Blood 1992; 80: 879–886.

    Google Scholar 

  20. Leibovici J. A model for the study of the long duration pathogenesis of cancer and its relevance to therapy. Medical Hypoth 1983; 10: 105–112.

    Google Scholar 

  21. Leibovici J. Serial passage of tumors in mice in the study of tumor progression and testing of antineoplastic drugs. Cancer Res 1984; 44: 1981–1984.

    Google Scholar 

  22. Michowitz M, Klein O, Sharabi Y, Siegal A, Leibovici J. AKR lymphoma malignancy variants differing at late phase of metastasis. Cancer Lett 1990; 52: 39–47.

    Google Scholar 

  23. Leibovici J, Klein O, Argaman H, Klorin G, Michowitz M. Differential metastatic capacity of three AKR lymphoma variants. Int J Exp Pathol 1992; 73: 273–286.

    Google Scholar 

  24. Leibovici J, Stark Y, Kopel S. Different biological behavior of AKR lymphoma cells from primary and metastatic tumors. Experientia 1985; 41: 404–407.

    Google Scholar 

  25. Leibovici J, Zur-Lerman A, Kay S, Donin N, Barot R. Differentiation antigens and DNA cytofluorimetric pattern in relation to tumor progression in a murine T lymphoma. XXIst Meeting of the International Society for Oncodevelopmental Biology and Medicine. Jerusalem, Israel, 1993: 60.

  26. Leibovici J, Avichezer D, Gibboa-Garber N. Effect of lectins on tumorigenicity of AKR lymphoma cells of varying malignancy. Anticancer Res 1986; 6: 1411–1416.

    Google Scholar 

  27. Leibovici J, Klorin G, Huszar M, Hoenig S, Klein O, Michowitz M, Pinchassov A. Sensitivity to thermochemotherapy of AKR lymphoma and B16 melanoma variants of malignancy. Clin Exp Metastasis 1990; 8: 33–46.

    Google Scholar 

  28. Leibovici J, Michowitz M, Argaman H. Change in the role of the spleen from protective to harmful following tumor progression in AKR Lymphoma. Invasion Metastasis 1990; 10: 289–300.

    Google Scholar 

  29. Vindelov LL, Christensen LJ, Nissen NI. Standardisation of high-resolution flow cytometric DNA analysis by the simultaneous use of chicken and trout red blood cells as internal reference standard. Cytometry 1983; 3: 328–331.

    Google Scholar 

  30. Weiss L. Metastasis differences between cancer cells in primary and secondary tumors. Pathobiol Ann 1980; 10: 51–81.

    Google Scholar 

  31. Arends MJ, Wyllie AH. Apoptosis: mechanisms and roles in pathology. Int Rev Exp Pathol 1991; 32: 223–254.

    Google Scholar 

  32. Carson D, Ribeiro JM. Apoptosis and disease. Lancet 1993; 341: 1251–1254.

    Google Scholar 

  33. Rowan S, Ludwig RL, Haupt Y, Bates Y, Lu X, Oren M, Vousden KR. Specific loss of apoptotic but not cell cycle arrest function in a human tumor derived p53 mutant. EMBO J 1996; 15: 827–838

    Google Scholar 

  34. Majno G, Joris I. Apoptosis, oncosis and necrosis: an overview of cell death. Am J Pathol 1995; 146: 3–15.

    Google Scholar 

  35. La Biche RA, Tressler RJ, Nicolson GL. Selection for enhanced adhesion to microvessel endothelial cells or resistance to interferon-gamma modulates the metastatic potential of murine RAW117 large-cell-lymphoma cells. Clin Exp Metastasis 1993; 11: 472–481.

    Google Scholar 

  36. Klein O, Savion N, Staroselsky A, Leibovici J. Cellular functions related to metastasis differing between low-and high-malignancy variants of AKR lymphoma. Pathobiology 1992; 60: 157–162.

    Google Scholar 

  37. Gilletti S, Raz A. Autocrine motility factor is a growth factor. Biochim Biophys Res Commun 1993; 194: 446–457.

    Google Scholar 

  38. Garbisa S, Giovanni C, Biagini G, et al. Different metastatic aggressiveness in murine TS/A clones: ultrastructure extracellular glycoproteins and type IV collagenolytic activity. Invasion Metastasis 1988; 8: 177–192.

    Google Scholar 

  39. Yeatman TJ, Nicolson GL. Molecular basis of tumor progression: mechanisms of organ specific tumor metastasis. Semin Surg Oncol 1993; 9: 256–263.

    Google Scholar 

  40. Raff MC, Barres BA, Burne JF, Coles HSR, Ishizaki Y, Jacobson MD. Programmed cell death and the control of cell survival. Phil Trans R Soc Lond B 1994; 345: 265–268.

    Google Scholar 

  41. Wyllie AH. Death from inside out: an overview. Phil Trans R Soc Lond B 1994; 345: 237–241.

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel

    N. Donin, D. Katzenelson, J. Ravia, J. Hiss, G. Schiby, S. Kay, J. Sinai, M. Michowitz & J. Leibovici

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  1. N. Donin
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  2. D. Katzenelson
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  3. J. Ravia
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  4. J. Hiss
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  5. G. Schiby
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  6. S. Kay
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  7. J. Sinai
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  8. M. Michowitz
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  9. J. Leibovici
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Donin, N., Katzenelson, D., Ravia, J. et al. Apoptosis, cell proliferation and in vivo biological behaviour of primary and metastatic tumour cells of an AKR lymphoma variant. Apoptosis 2, 214–220 (1997). https://doi.org/10.1023/A:1026424817392

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  • DOI: https://doi.org/10.1023/A:1026424817392

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