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Squalene and other unsaturated hydrocarbons, inBryocladia cuspidata

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Summary

By gas chromatography-electron impact mass spectrometry the red algaBryocladia cuspidata, was found to contain n-pentadecane and n-heptadecane. Gas chromatography-chemical ionization mass spectrometry indicated the presence of squalene as well as a C21 pentaene and a C21 hexaene, tentatively identified as 1,6,9,12,15-heneicosapentaene and 1,6,9,12,15,18-heneicosahexaene.

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References

  1. The alga was tentatively identified by reference to W. R. Taylor, in: Marine Algae of the Eastern Tropical and Subtropical Coasts of the Americas, p. 586. Univ. of Michigan Press, Ann Arbor 1972. The identification was confirmed by R. B. Setzer, Allan Hancock Foundation Herbarium, University of Southern California, Los Angeles, Cal. 90007, USA.

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  2. B. cuspidata was extracted by the previously described procedure. The wet alga (5.0 g) was homogenized in a mixture of methanol-toluene (3∶1) and centrifuged. Sodium nitrate solution was added and the resulting toluene layer was separated by centrifugation and dried to give 0.7% of lipid; L. P. Hager, R. H. White, P. F. Hollenberg, D. L. Doubek, R.C. Brusca and R. Guerrero, in: Food-Drugs from the Sea Proceedings 1974, p. 421. Ed. H. H. Webber and G. D. Ruggieri. Marine Technology Society, Washington 1976.

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  3. We thank Dr L. P. Hager and Dr. D. L. Doubek (Department of Biochemistry, University of Illinois) for collecting the alga.

  4. W. W. Youngblood and M. Blumer, Mar. Biol.21, 163 (1973).

    Article  CAS  Google Scholar 

  5. W. W. Youngblood, M. Blumer, R. L. Guillard, and F. Fiore, Mar. Biol.8, 190 (1971).

    Article  CAS  Google Scholar 

  6. R. C. Clark, and M. Blumer, Limnol. Oceanogr.12, 79 (1967).

    Article  CAS  Google Scholar 

  7. S. Caccamese, K. L. Rinehart, Jr, P. D. Shaw and R. D. Johnson, 170th nat. Meet. Am. chem. Soc. Chicago 1975, Abstracts, ORGN 19.

  8. A Varian MAT mass spectrometer, Model 112, equipped with a chemical ionization ion source and interfaced to a Varian SS 100 computer system, was coupled to a Varian gas chromatograph, Model 1740.

  9. T. G. Halsall and I. R. Hills, Chem. Commun.1971, 448.

  10. R. F. Lee, J. C. Nevenzel, G. A. Paffenhöfer, A. A. Benson, S. Patton and T. E. Kavanaugh, Biochim. Biophys. Acta202, 386 (1970).

    Article  CAS  PubMed  Google Scholar 

  11. M. Blumer, M. M. Mullin and R. R. L. Guillard, Mar. Biol.6, 226 (1970).

    Article  CAS  Google Scholar 

  12. R. F. Lee and A. R. Loeblich III, Phytochemistry10, 593 (1971).

    Article  CAS  Google Scholar 

  13. J. S. Warner, Analyt. Chem.48, 578 (1976).

    Article  CAS  Google Scholar 

  14. The presence of C3H7+ ions in the reactant gas is shown by the weak peaks at m/e 329 for component C and m/e 327 for component D.

  15. F. H. Field, J. Am. chem. Soc.90, 5649 (1968).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Roger Adams Laboratory, University of Illinois, 61801, Urbana, Illinois, USA

    S. Caccamese & K. L. Rinehart Jr

Authors
  1. S. Caccamese
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  2. K. L. Rinehart Jr
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Additional information

Acknowledgments. This study was supported in part by a grant (AI 04769) from the National Institute of Allergy and Infectious Diseases. S.C. on leave of absence from the University of Catania, thanks the National Research Council of Italy for a Fellowship (1974–1975).

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Caccamese, S., Rinehart, K.L. Squalene and other unsaturated hydrocarbons, inBryocladia cuspidata. Experientia 34, 1129–1130 (1978). https://doi.org/10.1007/BF01922909

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