Abstract
The effect of the monovalent carboxylic ionophore monensin, which mediates a one-for-one exchange of intracellular H+ for extracellular Na+, was investigated in purified rat peritoneal mast cells. Monensin inhibited histamine secretion induced by compound 48/80, adriamycin and the calcium ionophore A23187; the inhibitory effect was maximal when the compound was added at least 10 min before the secretagogues. Washing of cells before addition of the secretagogues did not abolish the inhibitory effect of monensin. On the contrary the carboxylic ionophore was completely ineffective in preventing concanavalin A-induced histamine release.
When rat peritoneal mast cells were incubated in the presence of monensin for longer period (up to 5 hours), the substance induced a slow, progressive and dose dependent histamine release, which, at least for lower doses was noncytotoxic. The secretory effect of monensin was still present if the ionophore was washed away after 10 min of incubation, and the incubation continued in drug-free medium. Monensin stimulated histamine secretion was strictly dependent on extracellular Na+ concentrations, and independent on extracellular Ca++.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
D. Lagunoff and T. W. Martin,Agents that release histamine from mast cells. Ann. Rev. Pharmacol. Toxicol.23, 331–351 (1983).
B. C. Pressman,Biological applications of ionophores. Ann. Rev. Biochem.45, 501–530 (1976).
B. C. Pressman and M. Fahim,Pharmacology and toxicology of monovalent carboxylic ionophores. Ann. Rev. Pharmacol. Toxicol.22, 465–490 (1982).
F. Di Virgilio and B. D. Gomperts,Ionophore monensin induces Na +-dependen secretion from rabbit neutrophils. Requirement for intracellular Ca2+ stores. Biochim. Biophys. Acta763, 292–298 (1983).
G. Decorti, F. Bartoli Klugmann, L. Candussio and L. Baldini,characterization of histamine secretion induced by anthracyclines in rat peritoneal mast cells. Biochem. Pharmacol.35, 1939–1942 (1986).
D. Lagunoff and A. Rickard,Methods for the study of rat peritoneal mast cell secretion InIn vitro methods for studying secretion, the secretory process. Vol. 3, pp. 13–28 (Eds. A. M. Poisner and J. M. Trifarò). Elsevier Science Publishers B.V., Amsterdam 1987.
P. A. Shore, A. Burkhalter and V. H. Cohn,A method for the fluorometric assay of histamine in tissues. J. Pharmacol. Exp. Ther.127, 182–186 (1959).
M. J. Brody, R. Hakanson, C. Owman and F. Sundler,An improved method for the hystochemical demonstration of histamine and other compounds producing fluorophores with o-phthaldialdehyde. J. Histochem. Cytochem.20, 945–948 (1972).
G. Decorti, F. Bartoli Klugmann, L. Candussio, A. Furlani, V. Scarcia and L. Baldini,Uptake of adriamycin by rat and mouse mast cells and correlation with histamine release. Cancer Res.49, 1921–1926 (1989).
N. Uchida, H. Smilowitz and M. L. Tanzer,Monovalent ionophores inhibit secretion of procollagen and fibronectin from cultured human fibroblasts. Proc. Natl. Acad. Sci. USA76, 1868–1872 (1979).
A. M. Tartakoff and P. Vassalli,Plasma cell immunoglobulin secretion. Arrest is accompanied by alterations of the Golgi complex. J. Exp. Med.146, 1332–1345 (1977).
H. Smilowitz,Monovalent ionophores inhibit acetylcholinesterase release from cultured chick embryo skeletal muscle cells. Mol. Pharmacol.16, 202–214 (1979).
A. C. Rustan, J. O. Nossen, T. Tefre and C. A. Drevon,Inhibition of very-low-density lipoprotein secretion by chloroquine, verapamil an monensin takes place in the Golgi complex. Biochim. Biophys. Acta930, 311–319 (1987).
A. M. Tartakoff and P. Vassalli,Comparative studies of intracellular transport of secretory proteins. J. Cell Biol.79, 696–707 (1978).
E. L. Watson, C. J. Farnham, J. Friedman and W. Farnham,Effects of monensin on amylase release from mouse parotid acini. Am. J. Physiol.240, C189-C192 (1981).
S. J. Suchard, F. A. Lattanzio, R. W. Rubin and B. C. Pressman,Stimulation of catecholamine secretion from cultured chromaffin cells by an ionophore-mediated rise in intracellular sodium. J. Cell Biol.94, 531–539 (1982).
R. L. Perlman, A. F. Cossi and L. W. Role,Mechanisms of ionophore-induced catecholamine secretion. J. Pharmacol. Exp. Ther.213, 241–246 (1980).
M. B. Feinstein, E. G. Henderson and R. I. Sha'afi,The effects of alterations of transmembrane Na + and K+ gradients by ionophores (nigericin, monensin) on serotonin transport in human blood platelets. Biochim. Biophys. Acta458, 284–295 (1977).
W. Siffert and J. W. N. Akkerman,Na +/H+ exchange as a modulator of platelet activation. Trends. Biochem. Sci.13, 148–151 (1988).
D. A. Lowe, B. P. Richardson, P. Taylor and P. Donatsch,Increasing intracellular sodium triggers calcium release from bound pools. Nature260, 337–338 (1976).
S. J. Galli, A. M. Dvorak and H. F. Dvorak,Basophils and mast cells: morphologic insights into their biology, secretory patterns, and function. Prog. Allergy34, 1–141 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Decorti, G., Bartoli, F., Crivellato, E. et al. Effect of the carboxylic ionophore monensin on histamine release from rat peritoneal mast cells. Agents and Actions 32, 253–260 (1991). https://doi.org/10.1007/BF01980882
Issue Date:
DOI: https://doi.org/10.1007/BF01980882