The primary structure of the J3 subunit of desulfovjbrio desulfuricans (atcc 27774) [nife] hydrogenase

Title:The primary structure of the J3 subunit of desulfovjbrio desulfuricans (atcc 27774) [nife] hydrogenase

Volume: 4 Issue: 2

Author(s): Ricardo Francol, Juan J. Calvetel*, Hubert H. Thole, Manfred Raida, Isabel Moura and Jose J. G. Moura

Affiliation:

• Institut fur Reproduktionsmedizin, Tierartzliche Hochschule, Bunteweg 15, 30559-Hannover, Germany

Abstract: The periplasmic [NiFe] hydrogenase isolated from Desulfovibrio (D.) desulfuricans (ATCC 27774) is a heterodimer of a 28 kDa (13) and a 60 kDa (a) subunit. Here we report the complete amino acid sequence of the small (13) polypeptide chain determined by Edman degradation of proteolytic fragments. Electrospray-ionization mass spectrometry of the native protein confirmed the sequencing results. The sequence is compared with that of D. gigas [NiFe] hydrogenase whose three-dimensional structure has been recently published.

Cite this article as:

Francol Ricardo, Calvetel J. Juan*, Thole H. Hubert, Raida Manfred, Moura Isabel and Moura J. G. Jose, The primary structure of the J3 subunit of desulfovjbrio desulfuricans (atcc 27774) [nife] hydrogenase, Protein & Peptide Letters 1997; 4 (2) . https://dx.doi.org/10.2174/092986650402221014110919