Issue 17, 2010
From the journal:

Soft Matter

Arrangement of Annexin A2 tetramer and its impact on the structure and diffusivity of supported lipid bilayers†

Kirstin Fritz,a   Georg Fritz,b   Barbara Windschiegl,c   Claudia Steinemd  and  Bert Nickel*a  

* Corresponding authors

a Department für Physik and CeNS, Ludwig-Maximilians-Universität, Geschwister-Scholl-Platz 1, München, Germany
E-mail: nickel@lmu.de
Fax: (+49) 89 2180-1452
Tel: (+49) 89 2180-1460

b Arnold Sommerfeld Center for Theoretical Physics und CeNS, Ludwig-Maximilians-Universität, München, Germany

c Department of Micro- and Nanotechnology, Technical University of Denmark, 4000 Roskilde, Denmark

d Institut für Organische und Biomolekulare Chemie, Georg August Universität, Göttingen, Germany

Abstract

Annexins are a family of proteins that bind to anionic phospholipid membranes in a Ca2+-dependent manner. Annexin A2 forms heterotetramers (Anx A2t) with the S100A10 (p11) protein dimer. The tetramer is capable of bridging phospholipid membranes and it has been suggested to play a role in Ca2+-dependent exocytosis and cell–cell adhesion of metastatic cells. Here, we employ X-ray reflectivity measurements to resolve the conformation of Anx A2t upon Ca2+-dependent binding to single supported lipid bilayers (SLBs) composed of different mixtures of anionic (POPS) and neutral (POPC) phospholipids. Based on our results we propose that Anx A2t binds in a side-by-side configuration, i.e., both Anx A2 monomers bind to the bilayer with the p11 dimer positioned on top. Furthermore, we observe a strong decrease of lipid mobility upon binding of Anx A2t to SLBs with varying POPS content. X-Ray reflectivity measurements indicate that binding of Anx A2t also increases the density of the SLB. Interestingly, in the protein-facing leaflet of the SLB the lipid density is higher than in the substrate-facing leaflet. This asymmetric densification of the lipid bilayer by Anx A2t and Ca2+ might have important implications for the biochemical mechanism of Anx A2t-induced endo- and exocytosis.

Graphical abstract: Arrangement of Annexin A2 tetramer and its impact on the structure and diffusivity of supported lipid bilayers

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Article information

DOI
https://doi.org/10.1039/C0SM00047G
Article type
Paper
Submitted
02 Mar 2010
Accepted
30 Apr 2010
First published
01 Jul 2010

Soft Matter, 2010,6, 4084-4094

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Arrangement of Annexin A2 tetramer and its impact on the structure and diffusivity of supported lipid bilayers

K. Fritz, G. Fritz, B. Windschiegl, C. Steinem and B. Nickel, Soft Matter, 2010, 6, 4084 DOI: 10.1039/C0SM00047G

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