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Detecting Mollicutes by PCR in goats in southwestern Bahia, Brazil

  • Veterinary Microbiology - Research Paper
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Abstract

Brazil has a herd of over 9 million goats, and the northeast of Brazil is home to over 93% of this herd. Caprine mycoplasmosis are widely disseminated worldwide, being highly contagious with high rates of morbidity and mortality, causing considerable economic loss to goat herders. In addition, there has been a lack of research using molecular testing to monitor the health and detect Mollicutes in this herd in Brazil. Therefore, the aim of this study is to associate animal management with the presence of the caprine origin Mollicutes in goats, in the southwest region of the state of Bahia, Brazil. A cross-sectional study was conducted on twelve farms, and statistical analyses were performed to identify associations between the presence of Mollicutes and the management of goats. Molecular testing identified Mollicutes class, Mycoplasma agalactiae (Ma) and M. conjunctivae (Mc), in the samples analyzed. Statistical associations were observed between animals from intensive livestock facilities and the presence of Mollicutes in nasal samples and dairy ranch animals and the presence of Mollicutes in ocular samples and animals from extensive ranching sites and positive results of Mollicutes in genital samples. We conclude that mycoplasmas are present in goat herds in the southwestern region of Bahia, which supports the need for more focused studies of mycoplasmas throughout the country. Our research also demonstrated the presence of two important opportunistic bacteria, Mc and Ma, and, to the best of our knowledge, this is the first time that M. conjunctivae was detected in Brazilian goats by molecular testing.

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Acknowledgements

We thank Aricelma P. França for invaluable technical assistance and AcademicEnglishSolutions.com for revising the English.

Funding

This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 09/54825-5) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Code 001).

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Author notes

  1. Regis Edgar Castilho Junior and Carlos Augusto Scacchetti de Almeida contributed equally to this work.

Authors and Affiliations

  1. Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sao Paulo, Professor Lineu Prestes Avenue, 2415, Butantã, São Paulo, 05508-900, Brazil

    Regis Edgar Castilho Junior, Carlos Augusto Scacchetti de Almeida, Natália C. Gaeta & Nilson Roberti Benites

  2. Instituto de Ciências Biomedicas, Universidade de Sao Paulo, Brazil, Professor Lineu Prestes Avenue, 2415, Butantã, São Paulo, 05508-900, Brazil

    Regis Edgar Castilho Junior, Carlos Augusto Scacchetti de Almeida, Verena M. Santos, Aline T. Amorim, Natália C. Gaeta, Izadora R. Souza, Maysa B Santos, Guilherme B. Campos, Lucas Miranda Marques & Jorge Timenetsky

  3. Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Hormindo Barros Street, 58, Candeias, Vitória da Conquista, Bahia, 45029-094, Brazil

    Guilherme B. Campos & Lucas Miranda Marques

  4. Setor de Caprino Ovinocultura, Departamento de Fitotecnia e Zootecnia, Universidade Estadual do Sudoeste da Bahia, Estrada do Bem querer, km 4, Vitoria da Conquista, Bahia, 45083-900, Brazil

    Luiz Eduardo Barreto de Souza & Jurandir Ferreira da Cruz

Authors
  1. Regis Edgar Castilho Junior
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  2. Carlos Augusto Scacchetti de Almeida
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  3. Verena M. Santos
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  8. Guilherme B. Campos
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Correspondence to Lucas Miranda Marques.

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Castilho Junior, R.E., de Almeida, C.A.S., Santos, V.M. et al. Detecting Mollicutes by PCR in goats in southwestern Bahia, Brazil. Braz J Microbiol 52, 1589–1595 (2021). https://doi.org/10.1007/s42770-021-00517-z

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  • DOI: https://doi.org/10.1007/s42770-021-00517-z

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