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RNA-sequencing attest increased sperm motility in bovine spermatozoa treated with ethanolic extract of Putranjiva roxburghii

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Abstract

In the course of time, scientific communities have a growing interest in understanding ethano medicines. The Putranjiva roxburghii, a native plant of the Indian Subcontinent is described as a “Child amulet tree” in Ayurveda. Based on the fact that this herbal medicine has an indispensable component of integrative medicine, the present study was planned to assess the effect of ethanolic dried extract of Putranjiva seeds on the motility of X and Y-bearing bovine spermatozoa. The in-vitro effect of seed extract diluted in S-TALP medium on bull semen has been evaluated by Computer Assisted Semen Analysis (CASA) shows a marked increase in the motility of spermatozoa. Motile and non-motile spermatozoa have been separated by glass wool column from the control as well as treated group. The X and Y-bearing sperm quantification have been carried out by droplet digital polymerase chain reaction (ddPCR). The extract didn’t exert any differential effect on the motility and viability of X and Y chromosome-bearing spermatozoa. The transcriptome profiling (RNA-Seq) identified 93 differentially expressed genes between the extract treated and control group. It unveils the up-regulation of CATSPER, AKAP3, SPAG, ADAM1B, ADAM2 and ADAM32 genes that are involved in increasing sperm motility. Transcriptome profile also unveil the expression of ZAR1, CYP17A1, APPL2, HOXB4 and SP9 genes involved with embryonic development processes in Putranjiva extract-treated motile spermatozoa. The results envisaged the medicinal value of Putranjiva herb on increased fertility due to combinatory effect like increased sperm motility and favourableness on embryogenesis. The study ruled out the possibility of herbs having any biased effect on the selection of either male or female-bearing spermatozoa in the bull.

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Data availability

Data generated and analysed under this study is avaible with Accession Numbers: PRJNA545120; GEO: GSE131849.

Abbreviations

P. roxburghii :

Putranjiva roxburghii

CASA:

Computer assisted semen analysis

KEGG:

Kyoto Encyclopedia of Genes and Genomes

MAPK:

Mitogen-activated protein kinase

FPKM:

Fragments per kilobase million

ddPCR:

Digital droplet polymerase chain reaction

S-TALP:

Sterile-Tyrode's albumin lactate pyruvate

VCL:

Curvilinear velocity

VAP:

Average path velocity

VSL:

Straight line velocity

LIN:

Linearity

STR:

Straightness

WOB:

Wobbling Index

BCF:

Beat-cross frequency

ALH:

Amplitude of lateral head displacement

DNC:

Dancing

DNM:

Dancing mean

MAPK:

Mitogen-activated protein kinase

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Acknowledgements

Authors wish to thank Anand Agricultural University for providing financial support and facilities to carry out the research work.

Funding

The present work is supported by Anand Agricultural University, Anand, Gujarat, India.

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Author notes

  1. Jignesh M. Italiya and Mayank R. Patel share equal authorship.

Authors and Affiliations

  1. Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India

    Jignesh M. Italiya, Bhumi K. Thakkar, Subhash J. Jakhesara & Prakash G. Koringa

  2. Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India

    Mayank R. Patel & Shiven S. Patel

  3. Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India

    Akash V. Golaviya

  4. Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, Gujarat, 382010, India

    Chaitanya G. Joshi

  5. Centre for Infectious Animal Diseases, Faculty of Tropical AgriSciences, Czech University of Life Sciences, Prague, Czech Republic

    Jignesh M. Italiya

Authors
  1. Jignesh M. Italiya
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  2. Mayank R. Patel
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Contributions

JMI—data curation; methodology, original draft; MRP—data curation; formal analysis, software, visualization; AVG—data curation; formal analysis, software, supervision, validation; SSP—data curation; formal analysis, software, validation; BKT—formal analysis, review and editing; SJJ—conceptualization, investigation, resources, supervision, validation; CGJ—investigation, resources, supervision, validation; PGK—conceptualization, funding acquisition, investigation, project administration, resources, supervision, validation, review and editing.

Corresponding author

Correspondence to Prakash G. Koringa.

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Conflict of interest

The authors declare that they have no conflict of interest in the publication, though two of the authors are Editorial board members of this journal.

Compliance with ethical standards

This article does not contain any studies involving animals.

Additional information

Accession numbers: PRJNA545120; GEO: GSE131849.

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Supplementary file1 (DOCX 33 KB)

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Italiya, J.M., Patel, M.R., Golaviya, A.V. et al. RNA-sequencing attest increased sperm motility in bovine spermatozoa treated with ethanolic extract of Putranjiva roxburghii. 3 Biotech 13, 33 (2023). https://doi.org/10.1007/s13205-022-03452-4

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