Abstract
Twenty-four microsatellite loci were isolated and characterized from the genome of Rasbora borapetensis. Flanking polymerase chain reaction primers were designed and used to amplify these loci in 32 individuals. All loci were polymorphic with allele numbers ranging from 2 to 27, observed heterozygosity from 0.031 to 1.000 and expected heterozygosity from 0.031 to 0.965. All loci conformed to the Hardy–Weinberg equilibrium and no evidence of null alleles was observed. Pairwise comparisons between alleles did not detect any linkage disequilibrium. The high level of polymorphisms observed in these microsatellite loci will enhance future investigations on the genetic differentiation and structure of populations of Rasbora borapetensis.
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Acknowledgments
This study was conducted in part as a cooperative survey with the Japan International Research Center for Agricultural Sciences (JIRCAS) and the Living Aquatic Resources Research Center (LARRC), Lao PDR. Our thanks go to Mses. Photsamon Phommachan and Phonenaphet Chanthasone for their assistance in the field research, to Mses. Kyoko Yamanoi and Ponthip Goto for their support in the chemical analyses, and to Dr. DeMar Taylor for his English grammar correction of the manuscript. This study was supported in part by a Grant-in-Aid for Scientific Research (C-23580340, B-22405030 and B-22380133) from the Japan Society for the Promotion of Science.
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Koizumi, N., Morioka, S., Mori, A. et al. Characterization of twenty-four polymorphic microsatellite loci of Rasbora borapetensis . Conservation Genet Resour 5, 711–713 (2013). https://doi.org/10.1007/s12686-013-9888-6
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DOI: https://doi.org/10.1007/s12686-013-9888-6