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Extraction of Inulinase Obtained by Solid State Fermentation of Sugarcane Bagasse by Kluyveromyces marxianus NRRL Y-7571

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Abstract

Production of inulinase by solid state fermentation always involves an extraction step, which dictates enzyme recovery yield and is related to cultivation conditions and control of process parameters. This work is focused on the study of extraction conditions aiming to maximize yield of an inulinase obtained by solid state fermentation of sugar cane bagasse and Kluyveromyces marxianus NRRL Y-7571. Kinetics of extraction was followed varying the kind of solvent used. After determining the best solvent, an experimental design was carried out to study the effect of the solid/liquid ratio (1:10–1:20), extraction temperature (20–53°C), and stirring rate (50–177 rpm). Results showed that maximum yield was obtained when sodium acetate buffer 0.1 M pH 4.8 was used, using a solid/liquid ratio of 1:10, at 53°C and 150 rpm for 40 min.

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References

  1. Gill, P. K., Manhas, R. K., & Singh, P. (2005). Bioresource Technology, 97, 894–902.

    Article  Google Scholar 

  2. Kim, C. H., & Rhee, S. K. (1989). Biotechnology Letters, 11, 201–206.

    Article  CAS  Google Scholar 

  3. Kalil, S. J., Suzan, R., Maugeri, F., & Rodrigues, M. I. (2001). Applied Biochemistry and Biotechnology, 94, 257–264.

    Article  CAS  Google Scholar 

  4. Silva-Santisteban, B. O. Y., & Maugeri, F. (2005). Enzyme and Microbial Technology, 36, 17–724.

    Article  Google Scholar 

  5. Selvakumar, P., & Pandey, A. (1999). Process Biochemistry, 34, 851–858.

    Article  CAS  Google Scholar 

  6. Mazutti, M. A., Bender, J. P., Treichel, H., & Di Luccio, M. (2006). Enzyme and Microbial Technology, 39, 56–59.

    Article  CAS  Google Scholar 

  7. Bender, J. P., Mazutti, M. A., Oliveira, D., Di Luccio, M., & Treichel, H. (2006). Applied Biochemistry and Biotechnology, 129–132, 951–958.

    Article  Google Scholar 

  8. Heck, J. X., Hertz, P. F., & Ayub, M. A. Z. (2005). Process Biochemistry, 40, 2891–2895.

    Article  CAS  Google Scholar 

  9. Singh, S. A., Ramakrishna, M., & Rao, A. G. A. (1999). Process Biochemistry, 35, 411–417.

    Article  CAS  Google Scholar 

  10. Castilho, L. R., Alves, T. L. M., & Medronho, R. A. (1999). Process Biochemistry, 34, 181–186.

    Article  CAS  Google Scholar 

  11. Castilho, L. R., Medronho, R. A., & Alves, T. L. M. (2000). Bioresource Technology, 71, 45–50.

    Article  CAS  Google Scholar 

  12. Mazutti, M., Ceni, G., Di Luccio, M., & Treichel, H. (2007). Bioprocess and Biosystems Engineering, 30(5), 297–304.

    Article  CAS  Google Scholar 

  13. Miller, G. L. (1959). Analytical Chemistry, 31, 426–428.

    Article  CAS  Google Scholar 

  14. Cruz-Guerrero, A., Garcia-Peña, I., Barzana, E., Racia-Garibay, M., & Gómez-Ruiz, L. Journal of Fermentation and Bioengineering, 80, 159–163.

  15. Pandey, A. (2003). Biochemical Engineering Journal, 13, 81–84.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Departamento de Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões Campus de Erechim, Av. Sete de Setembro 1621, Erechim, 99700-000, Brazil

    João Paulo Bender, Marcio Antônio Mazutti, Marco Di Luccio & Helen Treichel

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  1. João Paulo Bender
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  2. Marcio Antônio Mazutti
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  3. Marco Di Luccio
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  4. Helen Treichel
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Correspondence to Helen Treichel.

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Bender, J.P., Mazutti, M.A., Di Luccio, M. et al. Extraction of Inulinase Obtained by Solid State Fermentation of Sugarcane Bagasse by Kluyveromyces marxianus NRRL Y-7571. Appl Biochem Biotechnol 149, 195–203 (2008). https://doi.org/10.1007/s12010-007-8111-1

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  • DOI: https://doi.org/10.1007/s12010-007-8111-1

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