Abstract
Colored wheat has piqued the interest of breeders and consumers alike. The chromosomal segment from 7E of Thinopyrum ponticum, which carries a leaf rust resistant gene, Lr19, has been rarely employed in wheat breeding operations due to its association with the Y gene, which gives a yellow tint to the flour. By prioritizing nutritional content over color preferences, consumer acceptance has undergone a paradigm change. Through marker-assisted backcross breeding, we introduced an alien segment harboring the Y (PsyE1) gene into a high yielding commercial bread wheat (HD 2967) background to generate rust resistant carotenoid biofortified bread wheat. Agro-morphological characterization was also performed on a subset of developed 70 lines having enhanced grain carotene content. In the introgression lines, carotenoid profiling using HPLC analysis demonstrated a considerable increase in β-carotene levels (up to 12 ppm). Thus, the developed germplasm caters the threat to nutritional security and can be utilized to produce carotenoid fortified wheat.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
AACC (2000) Approved methods of the american association of cereal chemists, methods 14–50 (colorimetric method), 10th edn. American Association of Cereal Chemists, St. Paul, Minnesota
Alexandratos, N. and Bruinsma, J. (2012) World agriculture towards 2030/2050: the 2012 revision.
Álvarez D, Voß B, Maass D, Wüst F, Schaub P, Beyer P, Welsch R (2016) Carotenogenesis is regulated by 5′ UTR-mediated translation of phytoene synthase splice variants. Plant Physiol 172(4):2314–2326
Ceoloni C, Forte P, Kuzmanović L, Tundo S, Moscetti I, De Vita P, Virili ME, D’ovidio R (2017) Cytogenetic mapping of a major locus for resistance to Fusarium head blight and crown rot of wheat on Thinopyrum elongatum 7EL and its pyramiding with valuable genes from a Th. ponticum homoeologous arm onto bread wheat 7DL. Theor Appl Genet 130(10):2005–2024
Datta D, Prashar M, Bhardwaj SC, Singh S (2011) Alternate schemes for combining leaf rust resistance genes through molecular marker. Indian J Agric Sci 81(7):602
Fratianni A, Irano M, Panfili G, Acquistucci R (2005) Estimation of color of durum wheat. Comparison of WSB, HPLC, and reflectance colorimeter measurements. J Agric Food Chem 53(7):2373–2378
Garg M, Sharma N, Sharma S, Kapoor P, Kumar A, Chunduri V, Arora P (2018) Biofortified crops generated by breeding, agronomy, and transgenic approaches are improving lives of millions of people around the world. Frontiers in Nutrition, 12.
Garg M, Kaur S, Sharma A, Kumari A, Tiwari V, Sharma S, Kapoor P, Sheoran B, Goyal A, Krishania M (2022) Rising demand for healthy foods-anthocyanin biofortified rich colored wheat is a new research trend. Frontiers in Nutrition, p.913.
Gazza L, Galassi E, Ciccoritti R, Cacciatori P, Pogna NE (2016) Qualitative traits of perennial wheat lines derived from different Thinopyrum species. Genet Resour Crop Evol 63(2):209–219
Gennaro A, Borrelli GM, D’Egidio MG, De Vita P, Ravaglia S, Ceoloni C (2003). A chromosomally engineered durum wheat-Thinopyrum ponticum recombinant line with novel and promising attributes for varietal development. In Proceedings of the 10th international wheat genetic symposium (Vol. 2, pp. 881–883)
Giorio G, Stigliani AL, D’Ambrosio C (2008) Phytoene synthase genes in tomato (Solanumlycopersicum L.) – new data on the structures, the deduced amino acid sequences and the expression patterns. The FEBS Journal 275(3):527–535
Hefferon KL (2015) Nutritionally enhanced food crops; progress and perspectives. Int J Mol Sci 16(2):3895–3914
IBM Corp. Released (2017) IBM SPSS Statistics for Windows, Version 25.0. Armonk, NY: IBM Corp.
Knott DR (1989) The effect of transfers of alien genes for leaf rust resistance on the agronomic and quality characteristics of wheat. Euphytica 44(1):65–72
Kumar J, Saripalli G, Gahlaut V, Goel N, Meher PK, Mishra KK, Mishra PC, Sehgal D, Vikram P, Sansaloni C, Singh S (2018) Genetics of Fe, Zn, β-carotene, GPC and yield traits in bread wheat (Triticum aestivum L.) using multi-locus and multi-traits GWAS. Euphytica 214(11):1–17
Li J, Xuan J, Cai R (2020) Wheat, a popular cereal crop, Field Crop, 3.
Li H, Wang X (2009) Thinopyrum ponticum and Th. intermedium: the promising source of resistance to fungal and viral diseases of wheat. Journal of Genetics and Genomics 36(9):557–565
Matsumoto H, Ikoma Y, Kato M, Kuniga T, Nakajima N, Yoshida T (2007) Quantification of carotenoids in citrus fruit by LC-MS and comparison of patterns of seasonal changes for carotenoids among citrus varieties. J Agric Food Chem 55(6):2356–2368
Mishra DK, Gupta PK (1995) Protocol for evaluation of wheat quality. Technical Bulletin No.3. Karnal, India.
Mlalazi B, Welsch R, Namanya P, Khanna H, Geijskes RJ, Harrison MD, Harding R, Dale JL, Bateson M (2012) Isolation and functional characterisation of banana phytoene synthase genes as potential cisgenes. Planta 236(5):1585–1598
Monneveux P, Reynolds MP, Aguilar JG, Singh RP, Weber WE (2003) Effects of the 7DL. 7Ag translocation from Lophopyrum elongatum on wheat yield and related morphophysiological traits under different environments. Plant breeding 122(5):379–384
Newell-McGloughlin M (2008) Nutritionally improved agricultural crops. Plant Physiol 147(3):939–953
Nishino H, Tokuda H, Murakoshi M, Satomi Y, Masuda M, Onozuka M, Yamaguchi S, Takayasu J, Tsuruta J, Okuda M, Khachik F (2000) Cancer prevention by natural carotenoids. BioFactors 13(1–4):89–94
Nishino H, Murakoshi M, Ii T, Takemura M, Kuchide M, Kanazawa M, Yang Mou X, Wada S, Masuda M, Ohsaka Y, Yogosawa S (2002) Carotenoids in cancer chemoprevention. Cancer Metastasis Rev 21(3):257–264
Padhy AK (2019) Marker assisted selection in wheat for high carotenoid and protein content in grains. Available at: http://krishikosh.egranth.ac.in/handle/1/5810127613 (Accessed: February 20, 2022).
Padhy AK, Kaur P, Singh S, Kashyap L, Sharma A (2022) Coloured wheat and derived products: key to global nutritional security. Crit Rev Food Sci Nutr. https://doi.org/10.1080/10408398.2022.2119366
Padhy AK, Kaur P, Singh B, Kaur R, Bhatia S, Shamshad M, Sharma H, Kaur S, Srivastava P, Sharma A (2022a) In silico characterization of Thinopyrum elongatum-derived PsyE1 gene and validation in 7D/7E bread wheat introgression lines open avenues for carotenoid biofortification in wheat. Cereal Research Communications, pp.1–11. https://doi.org/10.1007/s42976-022-00279-w
Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res 26(5):496–500
R Core Team (2020) R: a language and environment for statistical computing. R Foundation for Statistical Computing
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci 84:8014–18
Sears ER (1972) Agropyron-wheat transfers through induced by homoeologous pairing. Can J Genet Cytol 14:736
Sears E R (1972b) Chromosome engineering in wheat, vol 4. Stadler Symposia, Columbia, pp 23–38.
Sharma D, Knott DR (1966) The transfer of leaf rust resistance from Agropyron to Triticum by irradiation. Can J Genet Cytol 8:137–143
Singh RP, Huerta-Espino J, Rajaram S, Crossa J (1998) Agronomic effects from chromosome translocations 7DL.7Ag and 1BL.1RS in spring wheat. Crop Sci 38:27–33
Singh G, Saini JS, Bains NS, Singh RP (2014) Positive influence of Lophopyrum ponticum derived Y gene on yellow pigment content-a major durum wheat quality trait. Indian J Genet Plant Breed 74:651–655
Wang C, Zeng J, Li Y, Hu W, Chen L, Miao Y, Deng P, Yuan C, Ma C, Chen X, Zang M (2014) Enrichment of provitamin A content in wheat (Triticum aestivum L.) by introduction of the bacterial carotenoid biosynthetic genes CrtB and CrtI. J exp bot 65(9):2545–2556
Weigand C (2011) Wheat import projections towards 2050. Arlington: US Wheat Associates.
Yang W, Mirbahar AA, Shoaib M, Lou X, Sun L, Sun J, Zhan K, Zhang A (2022) The carotenoid cleavage dioxygenase gene CCD7-B, at large, is associated with tillering in common wheat. Agriculture 12(2):306
Zeng J, Wang X, Miao Y, Wang C, Zang M, Chen X, Li M, Li X, Wang Q, Li K, Chang J (2015) Metabolic engineering of wheat provitamin A by simultaneously overexpressing CrtB and silencing carotenoid hydroxylase (TaHYD). J Agric Food Chem 63(41):9083–9092
Zhang W, Dubcovsky J (2008) Association between allelic variation at the Phytoene synthase 1 gene and yellow pigment content in the wheat grain. Theor Appl Genet 116(5):635–645
Zhang W, Lukaszewski AJ, Kolmer J, Soria MA, Goyal S, Dubcovsky J (2005) Molecular characterization of durum and common wheat recombinant lines carrying leaf rust resistance (Lr19) and yellow pigment (Y) genes from Lophopyrum ponticum. Theor Appl Genet 111(3):573–582
Zhou X, Welsch R, Yang Y, Álvarez D, Riediger M, Yuan H, Fish T, Liu J, Thannhauser TW, Li L (2015) Arabidopsis OR proteins are the major posttranscriptional regulators of phytoene synthase in controlling carotenoid biosynthesis. Proc Natl Acad Sci 112(11):3558–3563
Acknowledgements
The authors from Punjab Agricultural University are grateful to field/lab staff (Gagandeep Singh and Khushbir Kaur) for timely help in raising/maintenance of the crop and assistance in lab work. The authors from NIPGR are thankful to Sh. Sobha Ram for assistance in HPLC analysis.
Funding
The Department of Science and Technology, Government of India provided financial support under project, “Addressing Food Security through Nutritionally Enriched Improved Cultivars and Technologies for Swasth Bharat” under “Promotion of University Research and Scientific Excellence (PURSE) Grant” Grant number SR/PURSE Phase 2/25(G), 28.09.2017.
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Contributions
Conceiving and designing the study: Navtej Singh Bains and Achla Sharma.
Conduct of experiment: Asish Kumar Padhy and Himanshu Sharma.
Data analysis: Satinder Singh, Asish Kumar Padhy, Gurvinder Singh Mavi and Puja Srivastava.
Biochemical analysis: Harinderjit Kaur, Ashutosh Pandey, Ruchika Rajput and Lenika Kashyap.
Manuscript drafting: Achla Sharma, Asish Kumar Padhy, Satinder Singh, Satinder Kaur and Jaspal Kaur.
Editing and finalizing the manuscript: Virinder Singh Sohu, Parveen Chhuneja and Achla Sharma.
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Key message
First report of developing hexaploid wheat biofortified with carotenoid with a 3.5-fold increase than that of existing ones (3–4 ppm) and increased level of β-carotene (12 ppm) owing to introgression of Y gene (PsyE1) from Thinopyrum ponticum.
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Padhy, A.K., Sharma, A., Sharma, H. et al. Bread wheat with enhanced grain carotenoid content: a novel option for wheat biofortification. Mol Breeding 42, 67 (2022). https://doi.org/10.1007/s11032-022-01338-0
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DOI: https://doi.org/10.1007/s11032-022-01338-0