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Effects of sterilization on an extracellular matrix scaffold: Part I. Composition and matrix architecture

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Abstract

The impact of peracetic acid (PAA), lyophilization, and ethylene oxide (EO) sterilization on the composition and three dimensional matrix structure of small intestinal submucosa (SIS), a biologic scaffold used to stimulate the repair of damaged tissues and organs, was examined. Fibronectin and glycosaminoglycans are retained in SIS following oxidation by peracetic acid and alkylation using ethylene oxide gas. Significant amounts of FGF-2 are also retained, but VEGF is susceptible to the effects of PAA and is dramatically reduced following processing. Further, matrix oxidation, lyophilization, and sterilization with EO can be performed without irreversibly collapsing the three dimensional structure of the native SIS. These structural features and growth promoting extracellular matrix constituents are likely to be important variables underlying cellular attachment, infiltration and eventual incorporation of SIS into healing host tissues.

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Authors and Affiliations

  1. Cook Biotech Incorporated, 1425 Innovation Place, West Lafayette, IN, 47906

    Jason Hodde, Abram Janis, David Ernst, David Zopf & Chad Johnson

  2. Purdue University, Life Sciences Microscopy Facility, West Lafayette, IN, 47907

    Debra Sherman

Authors
  1. Jason Hodde
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  2. Abram Janis
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  3. David Ernst
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  4. David Zopf
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  5. Debra Sherman
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  6. Chad Johnson
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Correspondence to Jason Hodde.

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Hodde, J., Janis, A., Ernst, D. et al. Effects of sterilization on an extracellular matrix scaffold: Part I. Composition and matrix architecture. J Mater Sci: Mater Med 18, 537–543 (2007). https://doi.org/10.1007/s10856-007-2300-x

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  • DOI: https://doi.org/10.1007/s10856-007-2300-x

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