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First isolation and characterization of pteropine orthoreoviruses in fruit bats in the Philippines

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Abstract

Pteropine orthoreovirus (PRV) causes respiratory tract illness (RTI) in humans. PRVs were isolated from throat swabs collected from 9 of 91 wild bats captured on the Mindanao Islands, The Philippines, in 2013. The nucleic acid sequence of the whole genome of each of these isolates was determined. Phylogenetic analysis based on predicted amino acid sequences indicated that the isolated PRVs were novel strains in which re-assortment events had occurred in the viral genome. Serum specimens collected from 76 of 84 bats were positive for PRV-neutralizing antibodies suggesting a high prevalence of PRV in wild bats in the Philippines. The bat-borne PRVs isolated in the Philippines were characterized in comparison to an Indonesian PRV isolate, Miyazaki-Bali/2007 strain, recovered from a human patient, revealing that the Philippine bat-borne PRVs had similar characteristics in terms of antigenicity to those of the Miyazaki-Bali/2007 strain, but with a slight difference (e.g., growth capacity in vitro). The impact of the Philippine bat-borne PRVs should be studied in human RTI cases in the Philippines.

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Acknowledgements

We thank Yuto Suda and Momoko Ogata for their technical assistance. This work was supported in part by a Grant-in-aid from the Japan Society for the Promotion of Science KAKENHI (H25-Kiban-B-25304043), by a grant-in-aid from the Ministry of Health, Labour, and Welfare of Japan (H25-Shinko-Ippan-004, H26-Shinkogyousei-Shitei-002), JST/JICA SATREPS (Project: Project for ecological studies on flying foxes and their involvement in rabies-related and other viral infectious Diseases), and AMED (16fk0108101j0001).

Author information

Authors and Affiliations

  1. Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208–0011, Japan

    Satoshi Taniguchi, Kazutaka Egawa, Harpal Singh, Aiko Fukuma, Tomoki Yoshikawa, Hideki Tani, Shuetsu Fukushi, Masayuki Shimojima & Masayuki Saijo

  2. Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary MedicineYamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan

    Ken Maeda

  3. Department of Veterinary Microbiology, Graduate School of Agricultural and Life SciencesThe University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Taisuke Horimoto

  4. College of Veterinary Medicine, University of the Philippine Los Baños, Los Baños, 4031, Laguna, Philippines

    Joseph S. Masangkay

  5. University of the Philippines Mindanao, Bago-Oshiro, Davao, Philippines

    Roberto Puentespina Jr.

  6. Museum of Natural History, University of the Philippines Los Baños, Los Baños, 4031, Laguna, Philippines

    James Alvarez, Eduardo Eres & Edison Cosico

  7. Department of Pathology, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, 208-0011, Japan

    Noriyo Nagata

  8. Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan

    Shinobu Tsuchiaka, Tsutomu Omatsu & Tetsuya Mizutani

  9. Laboratory of Veterinary Pathology, School of Veterinary Medicine Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 252-5201, Japan

    Yumi Une

  10. Department of Animal Risk Management, Chiba Institute of Science, Choshi, Chiba, 288-0025, Japan

    Yasuhiro Yoshikawa

  11. Department of Biomedical Science, Graduate School of Agricultural and Life SciencesThe University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Shigeru Kyuwa

  12. Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan

    Masayuki Saijo

Authors
  1. Satoshi Taniguchi
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  2. Ken Maeda
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  3. Taisuke Horimoto
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  4. Joseph S. Masangkay
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  5. Roberto Puentespina Jr.
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  6. James Alvarez
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  7. Eduardo Eres
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  8. Edison Cosico
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  9. Noriyo Nagata
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  10. Kazutaka Egawa
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  11. Harpal Singh
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  12. Aiko Fukuma
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  13. Tomoki Yoshikawa
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  14. Hideki Tani
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  15. Shuetsu Fukushi
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  16. Shinobu Tsuchiaka
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  17. Tsutomu Omatsu
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  18. Tetsuya Mizutani
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  19. Yumi Une
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  20. Yasuhiro Yoshikawa
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  21. Masayuki Shimojima
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  22. Masayuki Saijo
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  23. Shigeru Kyuwa
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Corresponding author

Correspondence to Masayuki Saijo.

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Conflict of interest

The authors declare that they have no conflict of interest.

Wild bats were captured under a permit issued by the Department of Environment and Natural Resources (DENR) to the University of the Philippines Los Baños for this research purpose (Wildlife Gratuitous Permit No. WGP No. RXI-2013-06). Furthermore, for every scientific expedition undertaken by the authors to capture bats, a permit was issued by the Protected Areas and Wildlife Bureau (PAWB). Each scientific expedition to capture bats was also covered by a permit granted by the local regional office of the DENR. The procedures for oral swab and blood sample collection after euthanasia of the captured bats were carried out based on the guidance of the institutional animal care and use committee of the University of the Philippines Los Baños.

The patient serum sample was used in this study with informed consent and a written document [6] and in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Taniguchi, S., Maeda, K., Horimoto, T. et al. First isolation and characterization of pteropine orthoreoviruses in fruit bats in the Philippines. Arch Virol 162, 1529–1539 (2017). https://doi.org/10.1007/s00705-017-3251-2

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