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Chromosomal location of human genes encoding major heat-shock protein HSP70

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Somatic Cell and Molecular Genetics

Abstract

The HSP70 family of heat-shock proteins constitutes the major proteins synthesized in response to elevated temperatures and other forms of stress. In eukaryotes members of the HSP70 family also include a protein similar if not identical to bovine brain uncoating ATPase and glucose-regulated proteins. An intriguing relation has been established between expression of heat-shock proteins and transformation in mammalian cells. Elevated levels of HSP70 are found in some transformed cell lines, and viral and cellular gene products that are capable of transforming cells in vitro can also stimulate transcription of HSP70genes. To determine the organization of this complex multigene family in the human genome, we used complementary approaches: Southern analysis and protein gels of Chinese hamster-human somatic cell hybrids, and in situ hybridization to human chromosomes. We demonstrate that functional genes encoding HSP70 proteins map to human chromosomes 6, 14, 21, and at least one other chromosome.

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Authors and Affiliations

  1. Eleanor Roosevelt Institute for Cancer Research, 1899 Gaylord Street, 80206, Denver, Colorado

    Gail Singer Harrison, Harry A. Drabkin, Fa -Ten Kao, Judith Hartz & Iris M. Hart

  2. Division of Medical Oncology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, 80262, Denver, Colorado

    Gail Singer Harrison & Harry A. Drabkin

  3. Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, 80262, Denver, Colorado

    Fa -Ten Kao

  4. Department of Human Genetics, University of Michigan Medical School, 1137 East Catherine Street, 48109, Ann Arbor, Michigan

    Ernest H. Y. Chu

  5. Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2153 Sheridan Road, 60201, Evanston, Illinois

    Barbara J. Wu & Richard I. Morimoto

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  1. Gail Singer Harrison
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  2. Harry A. Drabkin
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  3. Fa -Ten Kao
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  4. Judith Hartz
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  6. Ernest H. Y. Chu
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  7. Barbara J. Wu
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  8. Richard I. Morimoto
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Harrison, G.S., Drabkin, H.A., Kao, F.T. et al. Chromosomal location of human genes encoding major heat-shock protein HSP70. Somat Cell Mol Genet 13, 119–130 (1987). https://doi.org/10.1007/BF01534692

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  • DOI: https://doi.org/10.1007/BF01534692

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