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The role of alcohol dehydrogenase in the fermentation of D-xylose byCandida shehatae ATCC 22984

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Summary

When the oxygen supply to an aerobic chemostat culture ofCandida shehatae growing on D-xylose was reduced to oxygen-limited and anoxic conditions, accumulation of ethanol occurred, the specific activity of alcohol dehydrogenase (ADH) increased up to four-fold and the number of ADH isozymes detected increased from one to three. ADH in crude extracts prepared from anoxically-grown cells had a lower affinity for but was more tolerant to ethanol than in extracts prepared from aerobically grown cells. ADH activity appeared to be sufficient for ethanol production byC. shehatae under anoxic conditions.

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Author notes

  1. Bernard A. Prior

    Present address: Department of Microbiology, University of the Orange Free State, P.O. Box 339, 9300, Bloemfontein, South Africa

Authors and Affiliations

  1. Institute of Microbial and Biochemical Technology, Forest Products Laboratory, 53705, Madison, Wisconsin, USA

    Bernard A. Prior, Vina Yang & Thomas W Jeffries

  2. Department of Chemical Engineering, University of Wisconsin, 53706, Madison, Wisconsin, USA

    Michael A. Alexander

Authors
  1. Bernard A. Prior
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  2. Michael A. Alexander
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  3. Vina Yang
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  4. Thomas W Jeffries
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Maintained in cooperation with the University of Wisconsin-Madison. The use of trade, firm or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the US Department of any product or service to the exclusion of others which may be suitable.

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Prior, B.A., Alexander, M.A., Yang, V. et al. The role of alcohol dehydrogenase in the fermentation of D-xylose byCandida shehatae ATCC 22984. Biotechnol Lett 10, 37–42 (1988). https://doi.org/10.1007/BF01030021

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