Abstract
Balloon flower (Platycodon grandiflorum A. DC) is widely distributed in South Korea and there are some local landraces that are cultivated as a vegetable crop or medicinal plant. Making use of the gene resources of wild-type and landraces is a way to increase the genetic diversity of the cultivars. However, few tools or information are available on an efficient identification system for maintaining and management of these landraces. To improve the genetic resources for balloon flower, 22 simple sequence repeat (SSR) markers, also known as microsatellite markers, were evaluated in a collection of 42 balloon flower landraces, 34 of which were from Korea and eight from China. All microsatellite markers produced the 107 alleles ranging from 2 to 10 with a mean of 4.864 alleles per each locus (NA). The values of observed heterozygosity (HO) and expected heterozygosity (HE) ranged from 0.00 to 0.667 (mean of 0.285) and from 0.024 to 0.741 (mean of 0.416), respectively. An average value of polymorphic information contents (PIC) were 0.382 with a range of 0.023 to 0.703. Results of population structure and phylogenetic and principal coordinate analysis (PCoA) indicated that P. grandiflorum germplasm formed two largely distinct clusters according to their origins and the genetic differentiation. There was a high level of genotypic diversity at broad geographic regions between Korea and China, but the low genetic differentiation was found within the collections from Korea. The results of the genetic diversity will be useful for the selection of the parents for developing balloon flower breeding and the multi-locus SSR markers developed herein will be a valuable resource for germplasm assessments, evaluation of genetic diversity, and population genetic studies of balloon flower.
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Cho YI, Park JH, Lee CW, Ra W-H, Chung J-W, Lee J-R, Ma KH, Lee S-Y, Lee K-S, Lee M-C, Park Y-J. 2011. Evaluation of the genetic diversity and population structure of sesame (Sesamum indicum L.) using microsatellite marker. Genes Genom. 33: 187–195
Evanno G, Regnaut S, Goudet J. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Mol. Ecol. 14: 2611–2620
Germida JJ, Siciliano SD, Freitas JR, Seib AM. 1998. Diversity of root-associated bacteria associated with field-grown canola (Brassica napus L.) and wheat (Triticum aestivum L.). FEMS Microbiol. Ecol. 26: 43–50.
Gupta PK, Varshney RK. 2000. The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113: 163–185
Hong DY, Lian YS, Shen LD. 1983. Flora Reipublicae Popularis Sinicae. Sci. Press, 73: 76–77
Kang YS, Hong KP, Jung DC, Hong S, Lee JH, Nah SY, Lim Y, Bae DH. 2008. Calcium channel-blocking activity of Chinese balloon flower (Platycodon grandiflorum) for producing blood pressure-lowering functional foods. Food Sci. Biotechnol. 17: 156–160
Kim KS, Seo EK, Lee YC, Lee TY, Cho YW, Ezaki O, Kim CH. 2000. Effect of dietary Platycodon grandiflorum on the improvement of insulin resistance in obese Zucker rats. J. Nutr. Biochem. 11: 420–424
Kim YS, Kim JS, Choi SU, Kim JS, Lee HS and Roh SH. 2005. Isolation of a new saponin and cytotoxic effect of saponins from the root of Platycodon grandiflorum on human tumor cell lines. Planta Med. 71: 566–568
Kump B, Javornik B. 1996. Evaluation of genetic variability among common buckwheat (Fagopyrum esculentum Moench) populations by RAPD markers. Plant Sci. 114: 149–158
Lee JY, Hwang WI, Lim ST. 2004 Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. J. Ethnopharmacol. 93: 409–415
Li J, Schulz B, Stich B. 2010. Population structure and genetic diversity in elite sugar beet germplasm investigated with SSR markers. Euphytica 175: 35–42
Liu K, Muse SV. 2005. PowerMarker: An integrated analysis environment for genetic marker analysis. Bioinformatics 21: 2128–2129
Mariac C, Luong V, Kapran I, Mamadou A, Sagnard F, Deu M, Ndjeunga J, Bezancon G, Pham J-L, Vigouroux Y. 2006. Diversity of wild and cultivated pearl millet accessions (Pennisetum glaucum [L.] R. Br.) in Niger assessed by microsatellite markers. Theor. Appl. Genet. 114: 49–58
Peakall R, Smouse PE. 2006. GENALEX 6: genetic analysis in Excel Population genetic software for teaching and research. Mol. Ecol. Notes 6: 288–295
Phumichai C, Doungchan W, Puddhanon P, Jampatong S, Grudloyma P, Kirdsri C, Chunwongse J, Pulam T. 2008. SSR-based and grain yield-based diversity of hybrid maize in Thailand. Field Crops Res. 108: 157–162
Pritchard JK, Stephens M, Donnelly P. 2000. Inference of population structure using multilocus genotype data. Genetics 155: 945–959
Pritchard JK, Wen X, Falush D. 2007. Documentation for structure software: Version 2.2. Department of Human Genetics, University of Chicago; Department of Statistics, University of Oxford. Available at http://pritch.bsd.uchicago.edu/software
Saxena B, Kaur R, Bhardwaj SV. 2011. Assessment of Genetic Diversity in Cabbage Cultivars Using RAPD and SSR Markers. J. Crop Sci. Biotech. 14: 191–196
Schuelke M. 2000. An economic method for the fluorescent labeling of PCR fragments. Nat. Biotechnol. 18: 233–234
Sirithunya P, Ukoskit K. 2010. Population Genetic Structure and Genetic Diversity of Jatropha curcas Germplasm as Investigated by 5′-anchored Simple Sequence Repeat Primers. J. Crop Sci. Biotech. 13: 147–153
Song JY, Lee G-A, Yoon M-U, Ma K-H, Choi Y-M, Lee JR, Park H-J, Lee M-C. (in press). Development and characterization of 22 polymorphic microsatellite markers for the balloon flower Platycodon grandiflorus (Campanulaceae). Genet. Mol. Res.
Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596–1599
Tani N, Yoshimaru H, Kawahara T, Hoshi Y, Nobushima F, Yasui T. 2006. Determination of the genetic structure of remnant Morus boninensis Koidz. trees to establish a conservation program on the Bonin Islands, Japan. BMC Ecol. 6: 14
Vignal A, Milan D, SanCristobal M, Eggen A. 2002. A review on SNP and other types of molecular markers and their use in animal genetics. Genet. Sel. Evol. 34: 275–305
Vigouroux Y, Mitchell S, Matsuoka Y, Hamblin M, Kresovich S, Stephen J, Smith C, Jaqueth J, Smith OS, Doebley J. 2005. An analysis of genetic diversity across the maize genome using microsatellites. Genetics 169: 1617–1630
Wei JH, Yang CM, Chen SL, Cheng HZ, Huang LQ. 2006. Analysis of genetic diversity of the cultivation and wild germplasms of Platycodon grandiflorum based RAPD markers. World Sci. Technol./Mod. Tradit. Chin. Med. Mater Medica 8: 37–41
Yan Y-Z, Wu J-R. 2007. Genetic Relationships of Platycodon grandiflorum Cultivars from East-Asian Area Using RAPD Markers. Bull. Bot. Res. 27: 308–312
Yang X, Quiros C. 1993. Identification and classification of celery cultivars with RAPD markers. Theor. Appl. Genet. 86: 205–212
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First two authors equally contributed: Gi-An Lee and Jae Young Song
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Lee, GA., Song, J.Y., Sung, J.S. et al. Analysis of population structure and genetic diversity in balloon flower (Platycodon grandiflorum (Jacq.) A. DC.) germplasm using Simple Sequence Repeat (SSR) markers. J. Crop Sci. Biotechnol. 15, 281–287 (2012). https://doi.org/10.1007/s12892-012-0067-1
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DOI: https://doi.org/10.1007/s12892-012-0067-1