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A Standardized Protocol for the Induction of Specific Social Fear in Mice

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References

  1. American Psychiatric Association. Diagnostic and statistical manual of mental disorders (DSM-5), 5th ed. American Psychiatric Publishing, Washington DC, 2013, pp 202–208.

    Book  Google Scholar 

  2. Leichsenring F, Leweke F. Social anxiety disorder. N Engl J Med 2017, 376: 2255–2264.

    Article  Google Scholar 

  3. Pelissolo A, Abou Kassm S, Delhay L. Therapeutic strategies for social anxiety disorder: where are we now? Expert Rev Neurother 2019, 19: 1179–1189.

    Article  CAS  Google Scholar 

  4. Xu H, Liu L, Tian Y, Wang J, Li J, Zheng J. et al. A disinhibitory microcircuit mediates conditioned social fear in the prefrontal cortex. Neuron 2019, 102: 668-682.e5.

    Article  CAS  Google Scholar 

  5. Siegmund A, Wotjak CT. A mouse model of posttraumatic stress disorder that distinguishes between conditioned and sensitised fear. J Psychiatr Res 2007, 41: 848–860.

    Article  Google Scholar 

  6. Niwa M, Matsumoto Y, Mouri A, Ozaki N, Nabeshima T. Vulnerability in early life to changes in the rearing environment plays a crucial role in the aetiopathology of psychiatric disorders. Int J Neuropsychopharmacol 2011, 14: 459–477.

    Article  Google Scholar 

  7. Yamamuro K, Bicks LK, Leventhal MB, Kato D, Im S, Flanigan ME, et al. A prefrontal-paraventricular thalamus circuit requires juvenile social experience to regulate adult sociability in mice. Nat Neurosci 2020, 23: 1240–1252.

    Article  Google Scholar 

  8. Saavedra-Rodríguez L, Feig LA. Chronic social instability induces anxiety and defective social interactions across generations. Biol Psychiatry 2013, 73: 44–53.

    Article  Google Scholar 

  9. Slattery DA, Uschold N, Magoni M, Bär J, Popoli M, Neumann ID, et al. Behavioural consequences of two chronic psychosocial stress paradigms: Anxiety without depression. Psychoneuroendocrinology 2012, 37: 702–714.

    Article  Google Scholar 

  10. Feng X, Zhao HY, Shao YJ, Lou HF, Zhu LY, Duan S, et al. Anxiolytic effect of increased NREM sleep after acute social defeat stress in mice. Neurosci Bull 2020, 36: 1137–1146.

    Article  Google Scholar 

  11. Tchenio A, Lecca S, Valentinova K, Mameli M. Limiting habenular hyperactivity ameliorates maternal separation-driven depressive-like symptoms. Nat Commun 2017, 8: 1135.

    Article  Google Scholar 

  12. Straube T, Kolassa IT, Glauer M, Mentzel HJ, Miltner WH. Effect of task conditions on brain responses to threatening faces in social phobics: an event-related functional magnetic resonance imaging study. Biol Psychiatry 2004, 56: 921–930.

    Article  Google Scholar 

  13. Scaini S, Belotti R, Ogliari A. Genetic and environmental contributions to social anxiety across different ages: a meta-analytic approach to twin data. J Anxiety Disord 2014, 28: 650–656.

    Article  Google Scholar 

  14. Stein MB, Chen CY, Jain S, Jensen KP, He F, Heeringa SG, et al. Genetic risk variants for social anxiety. Am J Med Genet B Neuropsychiatr Genet 2017, 174: 120–131.

    Article  CAS  Google Scholar 

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Acknowledgements

We thank the members of Prof. Han Xu’s laboratory for their valuable advice. We are grateful for the technical support by the Core Facilities of Zhejiang University Institute of Neuroscience. This work was supported by grants from the National Natural Science Foundation of China (32071005, 31471025, and 91432110), the National Key R&D Program of China (2016YFA0501000), and the Fundamental Research Funds for the Central Universities (2019QNA5001).

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Author notes

  1. Junqiang Zheng and Yuanyuan Tian have contributed equally to this work.

Authors and Affiliations

  1. Department of Neurobiology and Department of Neurology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China

    Junqiang Zheng, Yuanyuan Tian, Haifeng Xu, Linfan Gu & Han Xu

  2. NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Science and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, 310058, China

    Junqiang Zheng, Yuanyuan Tian, Haifeng Xu, Linfan Gu & Han Xu

Authors
  1. Junqiang Zheng
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  2. Yuanyuan Tian
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  3. Haifeng Xu
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  5. Han Xu
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Correspondence to Han Xu.

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Zheng, J., Tian, Y., Xu, H. et al. A Standardized Protocol for the Induction of Specific Social Fear in Mice. Neurosci. Bull. 37, 1708–1712 (2021). https://doi.org/10.1007/s12264-021-00754-0

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  • DOI: https://doi.org/10.1007/s12264-021-00754-0

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