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Biogenic Nanoparticles from Schwanniomyces occidentalis NCIM 3459: Mechanistic Aspects and Catalytic Applications

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Abstract

When cells of Schwanniomyces occidentalis NCIM 3459 were incubated with 1 mM tetrachloroauric acid (HAuCl4) or silver nitrate (AgNO3), cell-associated nanoparticles were obtained. Their presence was confirmed by scanning electron microscope observations. The cell-free supernatant (CFS) of the yeast mediated the synthesis of gold nanoparticles. On account of the difficulties associated with the use of cell-bound nanoparticles, further work was restricted to extracellular nanoparticles. It was hypothesized that the CFS contained thermostable biomolecule(s) that mediated metal reduction reactions. Extraction of the CFS with chloroform/methanol (2:1) and subsequent separation by preparative thin layer chromatography led to the activity-guided purification of a glycolipid. The glycolipid was hydrolyzed and the glycone (glucose) and aglycone components (palmitic acid and oleic acid) were identified by gas chromatography-mass spectrometry. The purified glycolipid mediated the synthesis of gold and silver nanoparticles that were characterized by using an X-ray diffractometer and transmission electron microscope (TEM). The extracellular nanoparticles displayed catalytic activities and reduced 4-nitroaniline to benzene-1,4-diamine. This paper thus highlights nanoparticle synthesis by a hitherto unreported yeast culture, identifies the biomolecules involved in the process, and describes a potential application of the nanostructures.

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Acknowledgments

This work was supported by funds from University Grants Commission under University with Potential for Excellence, Phase II. PTM thanks Council of Scientific and Industrial Research (CSIR), New Delhi, India, for Senior Research Fellowship.

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  1. Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune, 411007, India

    Pallavi Mohite, Mugdha Apte, Ameeta Ravi Kumar & Smita Zinjarde

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  1. Pallavi Mohite
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Mohite, P., Apte, M., Kumar, A.R. et al. Biogenic Nanoparticles from Schwanniomyces occidentalis NCIM 3459: Mechanistic Aspects and Catalytic Applications. Appl Biochem Biotechnol 179, 583–596 (2016). https://doi.org/10.1007/s12010-016-2015-x

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