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Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria

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Abstract

A novel halophilic actinomycete, strain H32T, was isolated from a Saharan soil sample collected in El-Oued province, south Algeria. The isolate was characterized by means of polyphasic taxonomy. Optimal growth was determined to occur at 28–32 °C, pH 6.0–7.0 and in the presence of 15–25 % (w/v) NaCl. The strain was observed to produce abundant aerial mycelium, which formed long chains of rod-shaped spores at maturity, and fragmented substrate mycelium. The cell wall was determined to contain meso-diaminopimelic acid and the characteristic whole-cell sugars were arabinose and galactose. The predominant menaquinones were found to be MK-10(H4) and MK-9(H4). The predominant cellular fatty acids were determined to be anteiso C17:0, iso-C15:0 and iso-C16:0. The diagnostic phospholipid detected was phosphatidylcholine. Phylogenetic analyses based on the 16S rRNA gene sequence showed that this strain formed a distinct phyletic line within the radiation of the genus Actinopolyspora. The 16S rRNA gene sequence similarity indicated that strain H32T was most closely related to ‘Actinopolyspora algeriensis’ DSM 45476T (98.8 %) and Actinopolyspora halophila DSM 43834T (98.5 %). Furthermore, the result of DNA–DNA hybridization between strain H32T and the type strains ‘A. algeriensis’ DSM 45476T, A. halophila DSM 43834T and Actinopolyspora mortivallis DSM 44261T demonstrated that this isolate represents a different genomic species in the genus Actinopolyspora. Moreover, the physiological and biochemical data allowed the differentiation of strain H32T from its closest phylogenetic neighbours. Therefore, it is proposed that strain H32T represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora saharensis sp. nov. is proposed. The type strain is H32T (=DSM 45459T=CCUG 62966T).

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Acknowledgments

The authors are grateful to Khaled Kherraz for soil sampling from El-Oued province, Gabriele Pötter (DSMZ) for growing A. saharensis cultures and for assistance with chemotaxonomical analyses and Bettina Sträubler for assistance with DNA–DNA hybridizations.

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Authors and Affiliations

  1. Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria

    Atika Meklat, Noureddine Bouras, Abdelghani Zitouni & Nasserdine Sabaou

  2. Département de Biologie, Faculté des sciences Agronomiques, Vétérinaires et Biologiques, Université Saâd Dahleb de Blida, Blida, Algeria

    Atika Meklat

  3. Laboratoire de Génie Chimique, UMR 5503 (CNRS/INPT/UPS), INPT-ENSAT, Université de Toulouse, 1 Avenue de l’Agrobiopôle, Auzeville Tolosane, BP 32607, 31326, Castanet-Tolosan, France

    Florence Mathieu & Ahmed Lebrihi

  4. Université Moulay Ismail, Marjane 2, BP 298, Meknes, Morocco

    Ahmed Lebrihi

  5. Leibniz Institute DSMZ, German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany

    Peter Schumann, Cathrin Spröer & Hans-Peter Klenk

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  1. Atika Meklat
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  2. Noureddine Bouras
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  3. Abdelghani Zitouni
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  4. Florence Mathieu
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  9. Nasserdine Sabaou
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Correspondence to Nasserdine Sabaou.

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Meklat, A., Bouras, N., Zitouni, A. et al. Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria. Antonie van Leeuwenhoek 103, 771–776 (2013). https://doi.org/10.1007/s10482-012-9859-z

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  • DOI: https://doi.org/10.1007/s10482-012-9859-z

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