Abstract
A gram-negative bacterium designated BN30T, which is motile with a polar flagellum, non-endospores forming, oxidase- and catalase-positive, was isolated from soybean root nodule. The organism is facultative anaerobic and surface-wrinkled rod. It can grow at 10–40 °C, pH 6–8 and 6 % (w/v) NaCl. BLASTn search based on 16S rRNA gene sequence revealed that the strain is closely related to Diaphorobacter aerolatus 8604S-37T, Alicycliphilus denitrificans K601T, Simplicispira limi EMB325T, Diaphorobacter nitroreducens NA10BT and Diaphorobacter oryzae RF3T, which all belonged to the family Comamonadaceae in class Betaproteobacteria. Phylogenetic analysis showed that the strain formed a firm clade with three Diaphorobacter species, being closest to Diaphorobacter aerolatus 8604S-37T with similarity of 98.64 %. DNA–DNA relatedness values between strain BN30T and five reference strains ranged from 11.5 to 35.9 %. All the results of phylogenetic analysis, chemotaxonomical data (predominant fatty acids are C16:0, sum feature 3, sum feature 8 and C17:0 cyclo; major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol; major quinone is Q-8; G+C of total DNA is 65.2 %), physiological and phenotypic results supported that BN30T represented a novel species within the genus Diaphorobacter. The name Diaphorobacter ruginosibacter was proposed, and the type strain is BN30T (=ACCC06116T = DSM 27467T).
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Acknowledgments
We are grateful to Professor Jean Euzéby for his helpful advice on the nomenclature; Professor Soon-Wo Kwon, for kindly supplying type strain Diaphorobacter aerolatus 8604S-37T; Professor Jie Gu and Ph. D Wei Sun for their supporting and help with the use of Biolog GN2 microplates. This research was financially supported by the 863 project of China (2012AA100402) and the National Science Foundation of China (31125007 and 31370142).
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Communicated by Erko Stackebrandt.
The GenBank accession number for the 16S rRNA gene sequence of strain BN30T is KC825337. The strain had been deposited in Leibniz-Institut Deutsche Sammlung von Mikroorganismen und Zellkulturen Gmbh (DSMZ) and Agricultural Culture Collection of China (ACCC) under deposition number DSM27467 and ACCC06116. Reference strains Diaphorobacter nitroreducens NA10BT, Diaphorobacter oryzae RF3T, Simplicispira limi EMB325T and Alicycliphilus denitrificans K601T were bought from DSMZ; Diaphorobacter aerolatus 8604S-37T was supplied kindly by Professor Kwon (National Academy of Agricultural Science, Republic of Korea).
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Wei, X.L., Han, M.S., Xia, C.C. et al. Diaphorobacter ruginosibacter sp. nov., isolated from soybean root nodule, and emended description of the genus Diaphorobacter . Arch Microbiol 197, 683–692 (2015). https://doi.org/10.1007/s00203-015-1102-7
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DOI: https://doi.org/10.1007/s00203-015-1102-7