Abstract
Objective: To identify the apoptotic cells in gastric MALT lymphoma and its relationship between bcl-2 and p53 gene expression. Methods: TdT-mediated dUTP biotin Nick End labeling (TUNEL) and immuno-histochemistry ABC method were used to display apoptotic cells and the gene protein expression of bcl-2 and p53 independently. Results: Apoptotic indices (AI) in high-grade MALT lymphomas were significantly higher than in mixed-grade group and low-grade group (P<0.05). Bcl-2 was expressed in 83% of low-grade tumors, 61.6% of the median-grade tumors and 43.7% of high-grade tumors. An inverse correlation was observed between the expression of bcl-2 and apoptotic indices. Only 27 cases were p53 positive. The frequency of p53 positivity was significantly increased as the histologic grade advanced (P<0.05). There was also an inverse correlation between the expression of bcl-2 and p53. Conclusion: Apoptosis may be important in tumors development and transmission. p53 and bcl-2 were important regulatory genes of apoptosis and may be associated with transformation from low- grade to high-grade lymphomas.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Isaacson PG. Gastrointestinal lymphoma. Human Pathology 1994; 25:1020.
Isaacson P, Wright DH. Malignant lymphoma of mucosa-associated lymphoid tissue. Cancer 1983; 52:1410.
Evan G, Littlewood T. A matter of life and cell death. Science 1998; 281:1317.
Isaacson PG, Wotherspoon AG, Diss TC, et al. Bcl-2 expression in lymphomas. Lancet 1991; 337:175.
Chan WK, Mole MM, Levison DA, et al. Nuclear and cytoplasmic bcl-2 expression in endometrial hyperplasia and adenocarcinoma. J Pathol 1995; 177:241.
Silvestrini R, veneoni S, Daidone MG, et al. The bcl-2 protein: a prognostic indicator strongly related to p53 protein in lymph node-negative breast cancer patients. J Nat Cancer Inst 1994; 86:499.
Dogusoy G, Karayel FA, Gener S, et al. Histopathologic features and expression of bcl-2 and p53 proteins in primary gastric lymphomas. Pathol Oncol Res 1999; 5:36.
Navratil E, Gaulard P, Kanavaros P, et al. Expression of the bcl-2 protein in B cell lymphomas arising from mucosa associated lymphoid tissue. J Clin Pathol 1995; 48:18.
Bobsing BA, Corver WE, Gorsira MCB, et al. Specificity of seven monoclonal antibodies against p53 evaluated with Weatern blotting, immunohistochemistry, confocal laser scanning, microscopy and flow cytometry. Cytometry 1997; 28:11.
Said JW, Barrera R, Shintaku IP, et al. Immuno-histochemical analysis of p53 expression in malignant lymphomas. Am J Pathol 1992; 141:1343.
Du M, Singh N, Husseuin A, et al. Positive correlation between apoptotic and proliferative indices in gastrointestinal lymphomas of mucosa-associated lymphoid tissue (MALT). J Pathol 1996; 178:379.
Polyak K, Xia Y, Zweier JL, et al. A model for p53-induced apoptosis. Nature 1997; 389:300.
Lu QL, Abel P, Foster CS, et al. Bcl-2: role in epithelial differentiation and oncogenesis. Hum Pathol 1996; 27:2095.
Mosnier JF, Perret AG, Vindimian M, et al. An immunhistochemical study of the simultaneous expression of bcl-2 and p53 oncoproteins in epithelial tumors of the colon and rectum. Arch Pathol Lab Med 1996; 120: 654.
Hoshida Y, Kusakabe H, Furukawa H, et al. Reassessment of gastric lymphoma in light of the concept of mucosa-associated lymphoid tissue lymphoma: analysis of 53 patients. Cancer 1997; 80:1151.
Haldar S, Negrini M, Monne M, et al. Down-regulation of bcl-2 by p53 in breast cancer cells. Cancer Res 1994; 54:2095.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Xiao, Hl., Wang, D., Li, Zp. et al. Detection of apoptotic cells and immunohistochemical study of bcl-2 and p53 gene protein in primary gastric mucosa-associated lymphoid tissue (MALT) lymphoma. Chin J Cancer Res 12, 224–228 (2000). https://doi.org/10.1007/BF02983473
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02983473