GLORIA — GEOMAR Library Ocean Research Information Access

1

Online Resource

Reevaluating the Role of Acanthamoeba Proteases in Tissue Invasion: Observation of Cytopathogenic Mechanisms on MDCK Cell Monolayers and Hamster Corneal Cells

Omaña-Molina, Maritza ; González-Robles, Arturo ; Iliana Salazar-Villatoro, Lizbeth ; [et al.]

Hindawi Limited ; 2013

In: BioMed Research International Vol. 2013 ( 2013), p. 1-13

add to mindlist on the mindlist

Details

In: BioMed Research International, Hindawi Limited, Vol. 2013 ( 2013), p. 1-13

Abstract: The morphological analysis of the cytopathic effect on MDCK cell monolayers and hamster cornea and qualitative and quantitative analyses of conditioned medium and proteases were evaluated and compared between two strains of Acanthamoeba genotype T4. Further than highlighting the biological differences found between both strains, the most important observation in this study was the fact that proteases both in total extracts and in conditioned medium are apparently not determinant in tissue destruction. An interestingly finding was that no lysis of corneal tissue was observed as it was previously suggested. These results, together with previous studies, allow us to conclude that the invasion and disruption of corneal tissue is performed by the penetration of the amoebae through cell junctions, either by the action of proteases promoting cellular separation but not by their destruction and/or a mechanical effect exerted by amoebae. Therefore, contact-dependent mechanisms in Acanthamoeba pathogenesis are more relevant than it has been previously considered. This is supported because the phagocytosis of recently detached cells as well as those attached to the corneal epithelium leads to the modification of the cellular architecture facilitating the migration and destruction of deeper layers of the corneal epithelium.

Type of Medium: Online Resource

ISSN: 2314-6133 , 2314-6141

URL: Article

DOI: 10.1155/2013/461329

Language: English

Publisher: Hindawi Limited

Publication Date: 2013

detail.hit.zdb_id: 2698540-8

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

2

Online Resource

Silicone Hydrogel Contact Lenses Surface Promote Acanthamoeba castellanii Trophozoites Adherence : Qualitative and Quantitative Analysis

Omaña-Molina, Maritza A. ; González-Robles, Arturo ; Salazar-Villatoro, Lizbeth ; [et al.]

Ovid Technologies (Wolters Kluwer Health) ; 2014

In: Eye & Contact Lens: Science & Clinical Practice Vol. 40, No. 3 ( 2014-05), p. 132-139

add to mindlist on the mindlist

Details

In: Eye & Contact Lens: Science & Clinical Practice, Ovid Technologies (Wolters Kluwer Health), Vol. 40, No. 3 ( 2014-05), p. 132-139

Type of Medium: Online Resource

ISSN: 1542-2321

URL: Article

DOI: 10.1097/ICL.0000000000000024

Language: English

Publisher: Ovid Technologies (Wolters Kluwer Health)

Publication Date: 2014

detail.hit.zdb_id: 2084291-0

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

3

Online Resource

Identification of Compound Heterozygous EVC2 Gene Variants in Two Mexican Families with Ellis–van Creveld Syndrome

Negrete-Torres, Nancy ; Chima-Galán, María del Carmen ; Sierra-López, Ernesto Antonio ; [et al.]

MDPI AG ; 2023

In: Genes Vol. 14, No. 4 ( 2023-04-09), p. 887-

add to mindlist on the mindlist

Details

In: Genes, MDPI AG, Vol. 14, No. 4 ( 2023-04-09), p. 887-

Abstract: Background: Ellis–van Creveld syndrome (EvCS) is an autosomal recessive ciliopathy with a disproportionate short stature, polydactyly, dystrophic nails, oral defects, and cardiac anomalies. It is caused by pathogenic variants in the EVC or EVC2 genes. To obtain further insight into the genetics of EvCS, we identified the genetic defect for the EVC2 gene in two Mexican patients. Methods: Two Mexican families were enrolled in this study. Exome sequencing was applied in the probands to screen potential genetic variant(s), and then Sanger sequencing was used to identify the variant in the parents. Finally, a prediction of the three-dimensional structure of the mutant proteins was made. Results: One patient has a compound heterozygous EVC2 mutation: a novel heterozygous variant c.519_519 + 1delinsT inherited from her mother, and a heterozygous variant c.2161delC (p.L721fs) inherited from her father. The second patient has a previously reported compound heterozygous EVC2 mutation: nonsense mutation c.645G 〉 A (p.W215*) in exon 5 inherited from her mother, and c.273dup (p.K92fs) in exon 2 inherited from her father. In both cases, the diagnostic was Ellis–van Creveld syndrome. Three-dimensional modeling of the EVC2 protein showed that truncated proteins are produced in both patients due to the generation of premature stop codons. Conclusion: The identified novel heterozygous EVC2 variants, c.2161delC and c.519_519 + 1delinsT, were responsible for the Ellis–van Creveld syndrome in one of the Mexican patients. In the second Mexican patient, we identified a compound heterozygous variant, c.645G 〉 A and c.273dup, responsible for EvCS. The findings in this study extend the EVC2 mutation spectrum and may provide new insights into the EVC2 causation and diagnosis with implications for genetic counseling and clinical management.

Type of Medium: Online Resource

ISSN: 2073-4425

URL: Article

DOI: 10.3390/genes14040887

Language: English

Publisher: MDPI AG

Publication Date: 2023

detail.hit.zdb_id: 2527218-4

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

4

Online Resource

Flavonoids Present in Propolis in the Battle against Photoaging and Psoriasis

Rivera-Yañez, Claudia Rebeca ; Ruiz-Hurtado, Porfirio Alonso ; Mendoza-Ramos, María Isabel ; [et al.]

MDPI AG ; 2021

In: Antioxidants Vol. 10, No. 12 ( 2021-12-19), p. 2014-

add to mindlist on the mindlist

Details

In: Antioxidants, MDPI AG, Vol. 10, No. 12 ( 2021-12-19), p. 2014-

Abstract: The skin is the main external organ. It protects against different types of potentially harmful agents, such as pathogens, or physical factors, such as radiation. Skin disorders are very diverse, and some of them lack adequate and accessible treatment. The photoaging of the skin is a problem of great relevance since it is related to the development of cancer, while psoriasis is a chronic inflammatory disease that causes scaly skin lesions and deterioration of the lifestyle of people affected. These diseases affect the patient’s health and quality of life, so alternatives have been sought that improve the treatment for these diseases. This review focuses on describing the properties and benefits of flavonoids from propolis against these diseases. The information collected shows that the antioxidant and anti-inflammatory properties of flavonoids play a crucial role in the control and regulation of the cellular and biochemical alterations caused by these diseases; moreover, flavones, flavonols, flavanones, flavan-3-ols, and isoflavones contained in different worldwide propolis samples are the types of flavonoids usually evaluated in both diseases. Therefore, the research carried out in the area of dermatology with bioactive compounds of different origins is of great relevance to developing preventive and therapeutic approaches.

Type of Medium: Online Resource

ISSN: 2076-3921

URL: Article

DOI: 10.3390/antiox10122014

Language: English

Publisher: MDPI AG

Publication Date: 2021

detail.hit.zdb_id: 2704216-9

SSG: 15,3

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

5

Online Resource

DataSheet_1.docx

Ponciano-Gómez, Alberto ; Valle-Solis, Martha Iris ; Campos-Aguilar, Myriam ; [et al.]

Frontiers Media SA ; 2022

In: Frontiers in Immunology Vol. 13 ( 2022-8-16)

add to mindlist on the mindlist

Details

In: Frontiers in Immunology, Frontiers Media SA, Vol. 13 ( 2022-8-16)

Abstract: The current pandemic generated by SARS-CoV-2 has led to mass vaccination with different biologics that have shown wide variations among human populations according to the origin and formulation of the vaccine. Studies evaluating the response in individuals with a natural infection before vaccination have been limited to antibody titer analysis and evaluating a few humoral and cellular response markers, showing a more rapid and intense humoral response than individuals without prior infection. However, the basis of these differences has not been explored in depth. In the present work, we analyzed a group of pro and anti-inflammatory cytokines, antibody titers, and cell populations in peripheral blood of individuals with previous SARS-CoV-2 infection using BNT162b2 biologic. Our results suggest that higher antibody concentration in individuals with an earlier disease could be generated by higher production of plasma cells to the detriment of the presence of memory B cells in the bloodstream, which could be related to the high baseline expression of cytokines (IL-6 and IL-10) before vaccination.

Type of Medium: Online Resource

ISSN: 1664-3224

URL: Article

DOI: 10.3389/fimmu.2022.946770

DOI: 10.3389/fimmu.2022.946770.s001

Language: Unknown

Publisher: Frontiers Media SA

Publication Date: 2022

detail.hit.zdb_id: 2606827-8

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

6

Online Resource

Antifungal Activity of Mexican Propolis on Clinical Isolates of Candida Species

Rivera-Yañez, Claudia Rebeca ; Ruiz-Hurtado, Porfirio Alonso ; Reyes-Reali, Julia ; [et al.]

MDPI AG ; 2022

In: Molecules Vol. 27, No. 17 ( 2022-09-01), p. 5651-

add to mindlist on the mindlist

Details

In: Molecules, MDPI AG, Vol. 27, No. 17 ( 2022-09-01), p. 5651-

Abstract: Infections caused by micro-organisms of the genus Candida are becoming a growing health problem worldwide. These fungi are opportunistic commensals that can produce infections—clinically known as candidiasis—in immunocompromised individuals. The indiscriminate use of different anti-fungal treatments has triggered the resistance of Candida species to currently used therapies. In this sense, propolis has been shown to have potent antimicrobial properties and thus can be used as an approach for the inhibition of Candida species. Therefore, this work aims to evaluate the anti-Candida effects of a propolis extract obtained from the north of Mexico on clinical isolates of Candida species. Candida species were specifically identified from oral lesions, and both the qualitative and quantitative anti-Candida effects of the Mexican propolis were evaluated, as well as its inhibitory effect on C. albicans isolate’s germ tube growth and chemical composition. Three Candida species were identified, and our results indicated that the inhibition halos of the propolis ranged from 7.6 to 21.43 mm, while that of the MFC and FC50 ranged from 0.312 to 1.25 and 0.014 to 0.244 mg/mL, respectively. Moreover, the propolis was found to inhibit germ tube formation (IC50 ranging from 0.030 to 1.291 mg/mL). Chemical composition analysis indicated the presence of flavonoids, including pinocembrin, baicalein, pinobanksin chalcone, rhamnetin, and biochanin A, in the Mexican propolis extract. In summary, our work shows that Mexican propolis presents significant anti-Candida effects related to its chemical composition, and also inhibits germ tube growth. Other Candida species virulence factors should be investigated in future research in order to determine the mechanisms associated with antifungal effects against them.

Type of Medium: Online Resource

ISSN: 1420-3049

URL: Article

DOI: 10.3390/molecules27175651

Language: English

Publisher: MDPI AG

Publication Date: 2022

detail.hit.zdb_id: 2008644-1

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

7

Online Resource

Autoevaluación de los profesores de clínica integral de medicina sobre su desempeño docente

Osornio-Castillo, Leticia ; Sánchez-Reyes, Acela ; Ríos-Saldaña, M. Refugio ; [et al.]

Universidad Nacional Autonoma de Mexico ; 2015

In: Investigación en Educación Médica Vol. 4, No. 16 ( 2015-10), p. 183-189

add to mindlist on the mindlist

Details

In: Investigación en Educación Médica, Universidad Nacional Autonoma de Mexico, Vol. 4, No. 16 ( 2015-10), p. 183-189

Type of Medium: Online Resource

ISSN: 2007-5057

URL: Article

DOI: 10.1016/j.riem.2015.04.003

Language: Spanish

Publisher: Universidad Nacional Autonoma de Mexico

Publication Date: 2015

detail.hit.zdb_id: 2834876-X

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

8

Online Resource

The Role of Propolis as a Natural Product with Potential Gastric Cancer Treatment Properties: A Systematic Review

Rivera-Yañez, Nelly ; Ruiz-Hurtado, Porfirio Alonso ; Rivera-Yañez, Claudia Rebeca ; [et al.]

MDPI AG ; 2023

In: Foods Vol. 12, No. 2 ( 2023-01-16), p. 415-

add to mindlist on the mindlist

Details

In: Foods, MDPI AG, Vol. 12, No. 2 ( 2023-01-16), p. 415-

Abstract: Gastric cancer is one of the most common, aggressive, and invasive types of malignant neoplasia. It ranks fifth for incidence and fourth for prevalence worldwide. Products of natural origin, such as propolis, have been assessed for use as new complementary therapies to combat cancer. Propolis is a bee product with antiproliferative and anticancer properties. The concentrations and types of secondary metabolites contained in propolis mainly vary according to the geographical region, the season of the year, and the species of bees that make it. The present study is a systematic review of the main articles related to the effects of propolis against gastric cancer published between 2011 and 2021 in the PubMed and Science Direct databases. Of 1305 articles published, only eight studies were selected; among their principal characteristics was the use of in vitro analysis with cell lines from gastric adenocarcinoma and in vivo murine models of the application of propolis treatments. These studies suggest that propolis arrests the cell cycle and inhibits proliferation, prevents the release of oxidizing agents, and promotes apoptosis. In vivo assays showed that propolis decreased the number of tumors by regulating the cell cycle and the expression of proteins related to apoptosis.

Type of Medium: Online Resource

ISSN: 2304-8158

URL: Article

DOI: 10.3390/foods12020415

Language: English

Publisher: MDPI AG

Publication Date: 2023

detail.hit.zdb_id: 2704223-6

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

9

Online Resource

Tumor-infiltrating regulatory T cells, CD8/Treg ratio, and cancer stem cells are correlated with lymph node metastasis in patients with early breast cancer

Solis-Castillo, Luis Alberto ; Garcia-Romo, Gina Stella ; Diaz-Rodriguez, Alvaro ; [et al.]

Springer Science and Business Media LLC ; 2020

In: Breast Cancer Vol. 27, No. 5 ( 2020-09), p. 837-849

add to mindlist on the mindlist

Details

In: Breast Cancer, Springer Science and Business Media LLC, Vol. 27, No. 5 ( 2020-09), p. 837-849

Type of Medium: Online Resource

ISSN: 1340-6868 , 1880-4233

URL: Article

DOI: 10.1007/s12282-020-01079-y

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2020

detail.hit.zdb_id: 2394259-9

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

10

Online Resource

Type 2 diabetes mellitus BALB/c mice are more susceptible to granulomatous amoebic encephalitis: Immunohistochemical study

Omaña-Molina, Maritza ; Sanchez-Rocha, Raquel ; Hernandez-Martinez, Dolores ; [et al.]

Elsevier BV ; 2017

In: Experimental Parasitology Vol. 183 ( 2017-12), p. 150-159

add to mindlist on the mindlist

Details

In: Experimental Parasitology, Elsevier BV, Vol. 183 ( 2017-12), p. 150-159

Type of Medium: Online Resource

ISSN: 0014-4894

URL: Article

DOI: 10.1016/j.exppara.2017.09.001

Language: English

Publisher: Elsevier BV

Publication Date: 2017

detail.hit.zdb_id: 1466937-7

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher