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Utilization and stability of vitamins in serum-containing and serum-free media in CHO cell culture (1990)

Kurano, Satoko ; Kurano, Norihide ; Leist, Christian ; [et al.]

Springer

Cytotechnology 4 (1990), S. 243-250

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ISSN: 1573-0778

Keywords: Chinese hamster ovary (CHO) cells ; vitamins ; ascorbic acid ; choline ; nicotinamide ; thiamine

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract The concentrations of four vitamins, ascorbic acid, nicotinamide, choline and thiamine were evaluated in the culture supernatant of Chinese hamster ovary (CHO) cells. The media used were α-modified Eagle's minimum essential medium (MEM-α) supplemented with 10% fetal calf serum, and a 1:1 mixture of Ham's F12 and Dulbecco's modified Eagle's medium (DME/F12), containing neither serum nor protein. The reference experiment without cells revealed instability of ascorbic acid and thiamine. Moreover, a significant amount of each vitamin decreased in the culture supernatant. The possibility of growth limitation by vitamin depletion is strongly suggested.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00563784

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