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  • 11
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In electrophysiological experiments involving intracellular recording from horizontal cells in the isolated retina of the roach, light adaptation of the retina has been shown to result in potentiation both of (1) the depolarizing component of biphasic chromaticity type S-potentials, and (2) the temporal frequency transfer functions of photopic luminosity type horizontal cells. Under identical light adaptation conditions, the number of spinules on horizontal cell dendrites positioned laterally at cone pedicle ribbon synapses, increase by some threefold. The latter effect occurs equally in pedicles of red- and green-sensitive cones. Thus, horizontal cells are ‘plastic’ in both structural and electrophysiological respects. Furthermore, since the two electrophysiological parameters studied depend on negative feedback from horizontal cells onto cones, the results suggest that it is the inhibitory synapses that are plastic and that spinules may be sites of the negative feedback interaction. Physiological and behavioural aspects of light-dependent horizontal cell plasticity are also discussed.
    Type of Medium: Electronic Resource
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  • 12
    ISSN: 1435-1463
    Keywords: Keywords: Serotonin ; voltammetry ; corticosterone ; stress ; rat.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary. The effect of daily repeated 10 min immobilization on the serotoninergic neurotransmission and serum corticosterone levels was studied. Male Lewis rats were immobilized for a 10 min period daily once or on 5 consecutive days. Serotoninergic neurotransmission was followed using differential in vivo pulse voltammetry with carbon fibre electrodes measuring extracellular 5-hydroxyindoleacetic acid (5-HIAA) levels. Recordings were performed in brain areas involved in the control of behaviour, mood, and stress response such as the frontal cortex, the hippocampal CA-3 and dentate gyrus, the striatum, and the raphe nuclei dorsalis (NRD) and medialis (MRN). The first immobilization resulted in an increase of the extracellular 5-HIAA levels in all areas under study, except the striatum where no reaction was observed. The major effect was recorded in the frontal cortex, showing an increase of about 400% as compared to control, which lasted for 3 h after the end of the immobilization period. Beginning on day 2 in all areas, except the striatum, a consecutive habituation to the stressor seemed to occur, since the stress-induced increase in the voltammetric signal was found to be reduced after consecutive immobilization. Serum corticosterone levels were measured directly after a single and after 5 daily immobilization periods. After single immobilization the serum corticosterone level was found to be about 270 ng/ml. After the 5th immobilization about 300 ng/ml were detected. These differences were not found to be significant. In summary, our data indicate that the serotonin metabolism shows habituation in nearly all brain areas after repeated immobilization, though the corticosterone level at the end of the immobilization period was comparable after single and repeated immobilization.
    Type of Medium: Electronic Resource
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  • 13
    Publication Date: 2014-03-28
    Description: Basement rocks exposed in the Acatlán Complex of the Mixteca terrane in southern Mexico record two tectonothermal events: (1) a Devonian–Mississippian (ca. 365–318 Ma) event, recording extrusion and exhumation of high-pressure rocks; and (2) an Early to Middle Permian (ca. 289–263 Ma) event, involving N-S dextral shearing, transtensional deformation, and local S-vergent thrusting in a magmatic arc environment. We document an additional, regionally significant, tectonothermal event during the Middle to Late Triassic recorded by 40 Ar/ 39 Ar step-heating laser-probe ages ranging from ca. 239 and 219 Ma ( cooling from ca. 525 °C to 300 °C) for amphibole, muscovite, and biotite from: (1) the Carboniferous Amarillo unit, consisting of medium-grade, metasedimentary rocks intruded by mafic dikes; and (2) the Pennsylvanian–Middle Permian, low-grade, clastic-calcareous, arc-related Tecomate Formation. U-Pb laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS) data yield an age of 339 ± 6 Ma for the youngest population of detrital zircon grains in the Amarillo unit. Lithogeochemical and Sm-Nd isotopic data for the Amarillo unit dikes are very similar to those of other Carboniferous meta-igneous rocks in the eastern and southwestern part of the Acatlán Complex, displaying affinities transitional between mid-ocean-ridge basalt (MORB) and continental tholeiites, and initial Nd ( t = 339 Ma) values from -6.6 to +6.4, indicating both depleted and enriched mantle sources, as well as variable contamination by continental crust or by subduction-derived fluids. The 40 Ar/ 39 Ar cooling ages coincide with an apparent hiatus in magmatic activity in southern Mexico, which is inferred to record a change from steep to flat subduction.
    Print ISSN: 1941-8264
    Electronic ISSN: 1947-4253
    Topics: Geosciences
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  • 14
    Publication Date: 2016-10-08
    Description: Magmatic activity in continental arcs is known to vary in a non-steady-state manner, with the mechanisms driving magmatic activity being a matter of ongoing discussion. Of particular importance is the question of what extent episodic magmatism in continental arcs is governed by external factors (e.g., plate motions) and internal factors (e.g., feedback processes in the upper plate). To test existing models for magmatic episodicity, which are mostly based on temporally and spatially limited records, this study uses large data sets of geochronological, geochemical, and plate kinematic data to document the Paleozoic to Mesozoic development of the North and South American Cordilleras in eight transects from British Columbia to Patagonia. The temporal distribution of U/Pb bedrock and detrital zircon ages, used as a proxy for timing of magmatic accretion, shows that some minima and maxima of zircon abundance are nearly synchronous for thousands of kilometers along the arc. Some age patterns are characterized by a periodicity of 50–80 Ma, suggesting a cyclic controlling mechanism. Other magmatic lulls or flare-ups find no equivalents in adjacent sectors, indicating that either discrete events or variable lag times may also be important in governing magmatic activity in continental arcs. Magma composition in Mexico, the Peninsular Ranges, and the Sierra Nevada varies episodically and proportionally with the temporal record of arc activity. During flare-up events, there is an increase in Sm/Yb, indicating deeper melting, and a decrease in Nd i , suggesting a higher degree of crustal assimilation. Geochemical scatter also increases during the initiation of flare-up events. Plate kinematic data provide a means of evaluating mantle heat input. The correlation between plate convergence rate and magmatic accretion varies for each sector, suggesting that different flare-ups or lulls likely reflect variable combinations of processes.
    Print ISSN: 0003-004X
    Electronic ISSN: 1945-3027
    Topics: Geosciences
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  • 15
    Publication Date: 2017-06-28
    Description: Author(s): J. Struckmeier, J. Muench, D. Vasak, J. Kirsch, M. Hanauske, and H. Stoecker In this paper, the generic part of the gauge theory of gravity is derived, based merely on the action principle and on the general principle of relativity. We apply the canonical transformation framework to formulate geometrodynamics as a gauge theory. The starting point of our paper is constituted ... [Phys. Rev. D 95, 124048] Published Tue Jun 27, 2017
    Keywords: General relativity, alternative theories of gravity
    Print ISSN: 0556-2821
    Electronic ISSN: 1089-4918
    Topics: Physics
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  • 16
    Publication Date: 2017-09-19
    Description: Biallelic mutations of three prime repair exonuclease 1 (TREX1) cause the lupus-like disease Aicardi–Goutières syndrome in which accumulation of a yet unknown endogenous DNA substrate of TREX1 triggers a cyclic GMP–AMP synthase-dependent type I IFN response and systemic autoimmunity. Products of reverse transcription originating from endogenous retroelements have been suggested to be a major substrate for TREX1, and reverse transcriptase inhibitors (RTIs) were proposed as a therapeutic option in autoimmunity ensuing from defects of TREX1. In this study, we treated Trex1 –/– mice with RTIs. The serum RTI levels reached were sufficient to block retrotransposition of endogenous retroelements. However, the treatment did not reduce the spontaneous type I IFN response and did not ameliorate lethal inflammation. Furthermore, long interspersed nuclear elements 1 retrotransposition was not enhanced in the absence of Trex1. Our data do not support the concept of retroelement-derived cDNA as key triggers of systemic autoimmunity in Trex1-deficient humans and mice and motivate the continuing search for the pathogenic IFN-inducing Trex1 substrate.
    Print ISSN: 0022-1767
    Electronic ISSN: 1550-6606
    Topics: Medicine
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  • 17
    Publication Date: 2018-06-02
    Description: Purpose: Somatic mutations in the human cytosolic isocitrate dehydrogenase 1 ( IDH1 ) gene cause profound changes in cell metabolism and are a common feature of gliomas with unprecedented predictive and prognostic impact. Fourier-transform infrared (FT-IR) spectroscopy addresses the molecular composition of cells and tissue and was investigated to deduct the IDH1 mutation status. Experimental Design: We tested the technique on human cell lines that were transduced with wild-type IDH1 or mutated IDH1 and on 34 human glioma samples. IR spectra were acquired at 256 positions from cell pellets or tissue cryosections. Moreover, IR spectra were obtained from fresh, unprocessed biopsies of 64 patients with glioma. Results: IDH1 mutation was linked to changes in spectral bands assigned to molecular groups of lipids and proteins in cell lines and human glioma. The spectra of cryosections of brain tumor samples showed high interpatient variability, for example, bands related to calcifications at 1113 cm –1 . However, supervised classification recognized relevant spectral regions at 1103, 1362, 1441, 1485, and 1553 cm –1 and assigned 88% of the tumor samples to the correct group. Similar spectral positions allowed the classification of spectra of fresh biopsies with an accuracy of 86%. Conclusions: Here, we show that vibrational spectroscopy reveals the IDH1 genotype of glioma. Because it can provide information in seconds, an implementation into the intraoperative workflow might allow simple and rapid online diagnosis of the IDH1 genotype. The intraoperative confirmation of IDH1 mutation status might guide the decision to pursue definitive neurosurgical resection and guide future in situ therapies of infiltrative gliomas. Clin Cancer Res; 24(11); 2530–8. ©2017 AACR . See related commentary by Hollon and Orringer, p. 2467
    Print ISSN: 1078-0432
    Electronic ISSN: 1557-3265
    Topics: Medicine
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  • 18
    Publication Date: 2012-09-01
    Description: In the Acatlán Complex of southern Mexico, a late Paleozoic assemblage, consisting of a gabbro-diorite-tonalite-trondhjemite suite (Totoltepec pluton) and clastic-calcareous metasedimentary rocks (Tecomate Formation), postdates collisional orogeny that resulted in the amalgamation of Pangea. This region offers a rare opportunity to examine assemblages developed at different crustal levels along the periphery of Pangea at the critical stage between amalgamation and breakup. The Totoltepec pluton consists of minor mafic-ultramafic rocks (306 ± 2 Ma; concordant U-Pb zircon analysis) that are marginal to the main mafic-felsic intrusion (289 ± 2 Ma). Geochemistry of the marginal rocks indicates an arc tholeiitic to calc-alkaline character with high large ion lithophile elements (LILEs)/high field strength elements (HFSEs), flat rare earth element (REE) patterns, and initial Nd values of +1.3 to +3.3. The younger Totoltepec phase exhibits a calc-alkaline trace-element geochemistry with flat to moderately fractionated light (L) REE–enriched patterns and initial Nd values of –0.8 to +2.6, which are also consistent with an arc environment. The Sm-Nd isotopic signature is more primitive compared to contemporaneous arc-related igneous rocks in southern Mexico, suggesting the pluton was emplaced in a less mature, outboard part of the arc, and/or along a fault conduit. The Tecomate Formation, as currently defined, is a composite of lithologically similar strata deposited in several fault-bounded basins ranging from Carboniferous to Early Permian in age. To the south of the Totoltepec pluton, the depositional age of the Tecomate Formation is tightly constrained in one section to ca. 300 Ma, but in another section, it is between ca. 288 and ca. 263 Ma. The Tecomate Formation rocks are interpreted to have been derived from a late Paleozoic arc based on (1) arc-related geochemistry, (2) Nd (t) values ranging from –5.6 to +0.3 (t = 288 Ma) that overlap those of the Totoltepec pluton, and (3) detrital zircons with predominantly Carboniferous–Permian ages. The Totoltepec and Tecomate units in the study area form part of a continental arc extending from Guatemala to California, which necessitates subduction of the paleo-Pacific oceanic lithosphere beneath the western margin of a Pangea-A configuration.
    Print ISSN: 0016-7606
    Electronic ISSN: 1943-2674
    Topics: Geosciences
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  • 19
    Publication Date: 2012-07-03
    Description: Background We previously reported the results of a phase II study for patients with newly diagnosed primary central nervous system lymphoma treated with autologous peripheral blood stem-cell transplantation (aPBSCT) and response-adapted whole-brain radiotherapy (WBRT). Now, we update the initial results. Patients and methods From 1999 to 2004, 23 patients received high-dose methotrexate. In case of at least partial remission, high-dose busulfan/thiotepa (HD-BuTT) followed by aPBSCT was carried out. Patients refractory to induction or without complete remission after HD-BuTT received WBRT. Eight patients still alive in 2011 were contacted and Mini-Mental State Examination (MMSE) and the European Organisation for Research and Treatment of Cancer quality-of-life questionnaire (QLQ)-C30 were carried out. Results Of eight patients still alive, median follow-up is 116.9 months. Only one of nine irradiated patients is still alive with a severe neurologic deficit. In seven of eight patients treated with HD-BuTT, health condition and quality of life are excellent. MMSE and QLQ-C30 showed remarkably good results in patients who did not receive WBRT. All of them have a Karnofsky score of 90%–100%. Conclusions Follow-up shows an overall survival of 35%. In six of seven patients where WBRT could be avoided, no long-term neurotoxicity has been observed and all patients have an excellent quality of life.
    Print ISSN: 0923-7534
    Electronic ISSN: 1569-8041
    Topics: Medicine
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  • 20
    Publication Date: 2013-03-19
    Description: In this study, mapping of ultrashort T 2 and T 2 * of acutely isolated porcine menisci at B 0  = 9.4 T was investigated. Maps of T 2 were measured from a slice through the pars intermedia with a spin echo-prepared two-dimensional ultrashort-TE T 2 mapping technique published previously. T 2 * mapping was performed by two-dimensional ultrashort-TE MRI with variable acquisition delay. The measured signal decays were fitted by monoexponential, biexponential and Gaussian-exponential fitting functions. The occurrence of Gaussian-like signal decays is outlined theoretically. The quality of the curve fits was visualized by mapping the value δ = abs(1 – χ 2 red ). For T 2 * mapping, the Gaussian-exponential fit showed the best performance, whereas the monoexponential and biexponential fits showed regionally high values of δ ( δ 〉 20). Interpretation of the Gaussian-exponential parameter maps was found to be difficult, because a Gaussian signal component can be related to mesoscopic (collagen texture) or macroscopic (slice profile, shim, sample geometry) magnetic field inhomogeneities and/or residual 1 H dipole–dipole couplings. It seems likely that an interplay of these effects yielded the observed signal decays. Modulation of the T 2 * signal decay caused by chemical shift was observed and addressed to fat protons by means of histology. In the T 2 measurements, no modulation of the signal decay was observed and the biexponential and Gaussian-exponential fits showed the best performance with comparable values of δ . Our results suggest that T 2 mapping provides the more robust method for the characterization of meniscal tissue by means of MRI relaxometry. However, mapping of ultrashort T 2 , as performed in this study, is time consuming and provides less signal-to-noise ratio per time than the mapping of T 2 *. If T 2 * mapping is used, pixel-wise monitoring of the fitting quality based on reduced χ 2 should be employed and great care should be taken when interpreting the parameter maps of the fits. Copyright © 2013 John Wiley & Sons, Ltd. Mapping of ultrashort T 2 and T 2 * of isolated porcine menisci was investigated. The signal decays were fitted by monoexponential (ME), biexponential (BIE) and Gaussian-exponential (GE) fitting functions. The occurrence of Gaussian-like T 2 * signal decays is theoretically outlined. The quality of the fit was pixel-wise visualized by mapping of δ  = abs(1 –  χ 2 red ). The GE and BIE fits showed the best performance. The results suggest that T 2 mapping is more robust than T 2 * mapping in the context of MRI relaxometry of menisci.
    Print ISSN: 0952-3480
    Electronic ISSN: 1099-1492
    Topics: Medicine
    Published by Wiley-Blackwell
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