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  • 11
    Electronic Resource
    Electronic Resource
    Periodontal ligament fibroblasts, preosteoblasts, and prechondrocytes express receptors for epidermal growth factor in vivo:A comparative radioautographic study (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 23 (1988), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Radioiodinated mouse epidermal growth factor (EGF) was used in light and electron microscopic radioautographic studies of the binding of EGF to various cells of young rats. High levels of bound EGF were noted on periodontal ligament fibroblasts, preosteoblasts, and prechondrocytes. Fibroblast in the oral mucosa, tail subepithelial connective tissue, and tail tendon demonstrated much lower levels of binding. Ultrastructural radioautography revealed that silver grains, indicative of radioiodinated EGF, were positioned adjacent to or over the plasma membranes of the cells at 5 minutes after injection of the growth factor. The significance of the high level of EGF receptors on periodontal ligament fibroblasts, comparable to the number observed on preosteocytes and prechondrocytes, is discussed in terms of the possible progenitor role of periodontal ligament fibroblasts for adjacent hard tissue-producing cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1988.tb01419.x
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  • 12
    Electronic Resource
    Electronic Resource
    Immunocytochemical in vivo localization of fibronectin-rich contact sites on fibroblasts of normal periodontal ligament and inflamed gingiva (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 23 (1988), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Fibroblast-to-matrix attachment sites were studied by routine electron microscopy and immunocytochemistry. Rabbit antibodies to beagle dog plasma fibronectin and sheep antirabbit antibodies conjugated with horseradish peroxidase or ferritin were used to localize fibronectin at fibroblast-to-matrix attachment sites. In fibroblasts of healthy periodontal ligament, the attachment sites consisted of rectangular patches of amorphous material juxtaposed to the external surface of the plasma membrane. At these sites, the cell membrane was more densely stained and the adjacent cytoplasm was characterized by increased density and a high concentration of cytoplasmic filaments. The extracellular plaques contained fibronectin. Morphometric analysis indicated that the attachment plaques were approximately 90 nm thick, 250 nm wide, and 550 nm long, and distributed uniformly over both the cell body and peripheral cytoplasmic processes. In inflamed gingiva, the attachment sites were larger, irregular in shape, and with greater amounts of extracellular amorphous material and fibronectin associated to the cell surface. Cytoplasmic filaments were more often bundled as stress fibers which terminated in fibronexus-type junctions with extracellular fibronectin-coated filaments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1988.tb01364.x
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  • 13
    Electronic Resource
    Electronic Resource
    Ultrastructural evidence of directed cell migration during initial cementoblast differentiation in root formation (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 23 (1988), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Root formation in 14-day-old Sprague-Dawley rats was studied by light and electron microscopy. Special attention was focused on initial cementoblast differentiation. Disruption of the epithelial root sheath appears to be a consequence of directed cell migration by cells of the dental follicle proper which undergo differentiation into precementoblasts. Precementoblasts rapidly develop polarity towards the dentin, exhibiting major cytoplasmic processes rich in cytoplasmic filaments. These processes grow toward and eventually contact the dentin matrix. It is suggested that the cells of the dental follicle proper are cementoblast precursors which respond to chemoattractant substances released from newly deposited dentin matrix- and/or basal lamina-associated material of root sheath origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1988.tb01371.x
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  • 14
    Electronic Resource
    Electronic Resource
    Radioautographic analysis of 3H-fucose utilization by fibroblasts of the periodontal ligament (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 21 (1986), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The secretion of 3labeld glycoproteins from periodontal ligament fibroblasts was, studied by light and electron microseopic radioautography. At 5, 10. and 20 minutes after intravenous injection, 3H-fucose was concentrated in Golgi cisternae and saccules. By 35 minutes, the label was dispersed to the cell periphery and extracellular matrix. At 8 hours after injection, almost all of the radioactive label was associated with the cell surface or the adjacent extracellular matrix. Labeled glycoprotein was contained in collagen secretion granules and appeared to be relesasd simultaneousley with the collagen precursors The distribution of 3H-fucose labeled material was uniform across the periodontal ligament.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1986.tb01439.x
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  • 15
    Electronic Resource
    Electronic Resource
    Comparative radioautographic study of the effects of L-azetidine-2-carboxylic acid on matrix secretion and golgi of the mouse incisor (1984)
    Springer
    Calcified tissue international 36 (1984), S. 409-420 
    Details
    ISSN: 1432-0827
    Keywords: LACA ; Odontoblasts and ameloblasts ; 3H-glycine ; Collagen secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The effect of a proline analog,l-azetidine-2-carboxylic acid (LACA), on protein matrix secretion by odontoblasts and ameloblasts was compared by light and electron microscopic radioautography after injection of3H-glycine in young mice. LACA inhibited the secretion of dentin matrix with consequent accumulation of3H-glycine labeled procollagen in the cisternae of the rough endoplasmic reticulum. In contrast, LACA had no apparent effect on ameloblasts as enamel matrix continued to be packaged in the Golgi apparatus and secreted from Tomes' process within 30 min after injection of the radioprecursor. Electron microscopy revealed that LACA did not cause any change in ameloblast ultrastructure but produced a marked alteration of the odontoblast Golgi complex. All odontoblast Golgi saccules and collagen secretion granules disappeared within 2 h after LACA administration. Odontoblast Golgi cisternae, however, appeared not to be affected. These observations confirm previous studies conducted in this laboratory showing that Golgi saccules in collagen-secreting cells are the initial staging areas for the formation of secretory granules. These results also indicate that a close correlation exists between form and function in the Golgi apparatus of collagen-secreting cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405353
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  • 16
    Electronic Resource
    Electronic Resource
    Calmodulin blocker inhibits Ca++-ATPase activity in secretory ameloblast of rat incisor (1987)
    Springer
    Cell & tissue research 248 (1987), S. 103-110 
    Details
    ISSN: 1432-0878
    Keywords: Secretory ameloblast ; Ca++-ATPase ; Na+-K+-ATPase ; Ultracytochemistry ; Calmodulin blocker, trifluoperazine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of the calmodulin blocker, trifluoperazine (TEP), on membrane-bound Ca++ -ATPase, Na+ -K+ -ATPase (EC 3.6.1.3.) and the ultrastructure of the enamel organ were investigated in the lower incisors of normal and TFP-injected rats. The rats, of about 100 g body weight, were given either 0.2 ml physiological saline or 100 μg TFP dissolved in 0.2 ml physiological saline through a jugular vein and fixed by transcardiac perfusion with a formaldehyde-glutaraldehyde mixture at 1 and 2 h after TFP administration. Non-decalcified sections of the enamel organ less than 50 μm in thickness, prepared from dissected lower incisors, were processed for the ultracytochemical demonstration of Ca++-ATPase and Na+-K+ -ATPase by the one-step lead method at alkaline pH. In control saline-injected animals the most intense enzymatic reaction of Ca++-ATPase was demonstrated along the plasma membranes of the entire cell surfaces of secretory ameloblasts. Moderate enzymatic reaction was also observed in the plasma membranes of the cells of stratum intermedium and papillary layer. Reaction precipitates of Na+-K+-ATPase activity were localized clearly along the plasma membranes of only the cells of stratum intermedium and papillary layer. The most drastic effect of TFP was a marked disappearance of enzymatic reaction of Ca++-ATPase from the plasma membranes of secretory ameloblasts, except for a weak persistent reaction in the basolateral cell surfaces of the infranuclear region facing the stratum intermedium. The cells of stratum intermedium and papillary layer, however, continued to react for Ca++-ATPase even after TFP treatment. Similarly, Na+-K+-ATPase activity in these cells was not inhibited by TFP administration. Ultrastructural examination of secretory ameloblasts revealed that administration of TFP caused no considerable cytological changes and did not act as a cytotoxic agent. These results suggest that secretory ameloblasts may have an active Ca++ transport system, which is modulated by an endogenous calmodulin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01239969
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  • 17
    Electronic Resource
    Electronic Resource
    Fate of annular gap junctions in the papillary cells of the enamel organ in the rat incisor (1986)
    Springer
    Cell & tissue research 246 (1986), S. 523-530 
    Details
    ISSN: 1432-0878
    Keywords: Enamel organ ; Papillary cell ; Annular gap junction ; Lysosome ; Cytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To investigate the mechanisms whereby annular gap junctions in the papillary cells of the enamel organ are degraded intracellularly, continuously growing rat incisors were examined by electron microscopy of routine thin sections as well as for the cytochemical localization of inorganic trimetaphosphatase activity. Routine thin-section analysis revealed small flat or undulated gap junctions, hemi-annular gap junctions between an invaginated cell process and a cell body, and fully internalized cytoplasmic annular gap junctions. Both hemi-annular and annular gap junctions usually contain various organelles and/or inclusions, such as mitochondria, endoplasmic reticulum, ribosomes, vesicles, and lysosomes in the cytoplasm confined by the junctional membranes. Annular gap junctions are sometimes fused with vesicular or tubulovesicular structures. Cytochemistry of inorganic trimetaphosphatase activity revealed an intense enzymatic reaction within a system of tubular structures and round or oval dense bodies. Both structures are believed to correspond to primary lysosomes. A part of the Golgi apparatus also shows a weak reaction. Although hemi-annular gap junctions never show enzymatic reaction, annular gap junctions sometimes contain reaction products throughout their interior cytoplasm and inclusions. Fusion of annular gap-junctional membranes with reaction-positive tubular structures is also observed. In one instance, revealed in serial sections, an annular gap junction was encircled entirely by a reaction-positive structure. These results suggest that cytoplasmic annular gap junctions are formed by endocytosis of hemi-annular gap junctional membranes from the cell surface and then degraded intracellularly by lysosomal enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215192
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  • 18
    Electronic Resource
    Electronic Resource
    Sequential events in the formation of collagen secretion granules with special reference to the development of segment-long-spacing-like aggregates (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 199 (1981), S. 309-320 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The formation and maturation of collagen secretion granules in periodontal ligament (PDL) fibroblasts of young male Balb-C mice were studied by electron microscopy and cytochemistry. The Golgi apparatus was composed of several dictyosomes, each consisting of a stack of five smooth-walled cisternae. Each cisterna was connected at both ends to a dilated saccule. The cisternae, with their associated pairs of saccules, underwent progressive maturational changes from the immature to mature face of each dictyosome. The most immature saccules (type 1) were large, spherical, and filled with loosely arranged short filamentous structures. These saccules were continuous with the first and second Golgi cisternae (counting from the immature side). Golgi saccules type 2 were ellipsoidal, associated with the third and fourth cisternae, and contained parallel, elongate filaments. The most mature saccules, type 3, were more rectangular and connected by a short fifth cisterna. They contained aggregated filamentous material in the form of eight segment-long-spacing (SLS)-like crystallites, one in the center and seven at the periphery, to form a basic secretory unit. As type 3 saccules matured, the interconnecting cisterna progressively shortened until the two saccules were nearly juxtaposed. At this time the shortened fifth cisterna split to give rise to two independent presecretory granules. By progressive condensation, presecretory granules matured into secretion granules that contained a densely packed SLS- like aggregate, within which individual crystallites were no longer discernable. Maturation of cisternae and saccules involved removal of membrane, apparently by the formation and detachment of coated vesicles. The staining reaction with silver methenamine and phosphotungstic acid increased over the procollagen as the saccules matured, indicating addition of carbohydrate moieties and possible crosslinkages. It is concluded that the formation and maturation of collagen secretion granules in PDL fibroblasts involves the packaging and further modification of eight SLS-like crystallites, which are secreted as a basic unit.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091990302
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  • 19
    Electronic Resource
    Electronic Resource
    Effects of L-azetidine-2-carboxylic acid on matrix secretion and Golgi structure in fibroblasts and osteoblasts of the mouse (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 212 (1985), S. 232-238 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The effect of a proline analogue, L-azetidine-2-carboxylic acid (LACA), on collagenous matrix secretion by periodontal ligament fibroblasts and alveolar bone osteoblasts was studied by light and electron microscopic radioautography after injection of 3H-glycine; 3H-glycine labeled material accumulated in the cisternae of the rough endoplasmic reticulum and was not secreted for over 4 hours.The Golgi complex of both fibroblasts and osteoblasts showed a marked alteration of its composition after LACA administration. All Golgi saccules and collagen secretion granules disappeared within 2 hours. Flattened Golgi cisternae were still present and appeared to be unaffected by the administration of LACA.These observations indicate that Golgi saccules in collagen-secreting cells are the initial staging areas for the formation of collagen secretory granules and that there is a close correlation between form and function in the Golgi apparatus of collagensecreting cells.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092120303
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  • 20
    Electronic Resource
    Electronic Resource
    Radioautographic demonstration of receptors for epidermal growth factor in various cells of the oral cavity (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 222 (1988), S. 191-200 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Mouse iodinated epidermal growth factor (EGF) was localized by light and electron microscopic radioautography in basal cells of oral epithelium, papillary cells of the enamel organ, periodontal ligament fibroblasts, preodontoblast precursor cells, and preosteoblasts of the alveolar bone of 13-day-old Sprague-Dawley rats. The specificity of binding in these cells was suggested by an observed reduction of about 90% in the labeling when excess unlabeled EGF was injected along with the 125I-EGF. In contrast, fully differentiated cells, such as ameloblasts, odontoblasts, and osteoblasts, were only poorly labeled. Quantitative analysis of the light microscopic radioautographs revealed that the papillary cells had the highest level of labeling (5.5 grains per 100 μm2 of cell area). The significance of the rather high labeling of the preosteoblasts of the alveolar bone and the fibroblasts of the periodontal ligament is unknown. However, the well-known effect of EGF in producing precocious eruption of teeth may be a consequence of an effect on these two cell types.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092220212
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