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1

Digitale Medien

Characterization of K Opioid Receptors in Neurosecretosomes from Bovine Posterior Pituitary (1987)

Pesce, Guido ; Lang, Michael A. ; Russell, James T. ; [weitere]

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 49 (1987), S. 0

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ISSN: 1471-4159

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: The binding properties of opioid receptors on isolated nerve terminals (neurosecretosomes) from bovine posterior pituitaries were characterized. Both [3H]etorphine and [3H]ethylketocyclazocine ([3H]EKC) showed high-affinity binding with complex binding isotherms, consistent with the presence of multiple classes of binding sites. [D-Ala2,D-Leu5]enkephalin showed no specific binding and failed to displace [3H]etorphine at high concentrations, indicating the absence of μ, δ, or benzomorphan (K2) sites. Mathematical modelling of the data suggested the presence of three classes of binding sites. The first was of high affinity with Kd values of 0.9 and 2.0 nM for etorphine and EKC, respectively. The second class of sites appeared to bindetorphine with a KD of 150 nM, and EKC with extremely low affinity (unmeasurable binding). The third class of sites was characterized by KD values of 7 and 2 μM for etorphine and EKC, respectively. These results indicate that the nerve terminals of bovine posterior pituitary contain opioid binding sites of the K type. Futhermore, these binding sites appear heterogeneous, consisting of at least two and possibly more subtypes or states.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1471-4159.1987.tb02882.x

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2

Digitale Medien

Comparative Studies of the Binding of Dimeric and Monomeric Enkephalins to Neuroblastoma-Glioma NG108–15 Cells (1985)

Krumins, Solveig A. ; Rodbard, David

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 45 (1985), S. 0

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ISSN: 1471-4159

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: Abstract: Binding activity of the enkephalin dimer [d-Ala2, Leu5-NH-CH2--]2 (DPE2) to NG108–15 hybrid cells was compared to that of the monomer [d-Ala2, Leu5]enkephalin amide (DALEA). At 25°C, the values of the apparent affinity constant for DPE2, measured to intact and lysed cells and membranes, was 5.0 (±0.09) × 109M−1 for n = 28 experiments, as compared to 0.9 (±0.08) × 109M−1 (n = 16) for DALEA. At 4°C, the binding affinity of DPE2 decreased by 43% and that of DALEA by 33%. An important difference between the binding of DPE2 and DALEA was that, after necessary corrections for difference in maximal “bindability” of the respective tritiated enkephalins, the molar binding capacity for DALEA was twofold higher than for DPE2, although mutual cross-displacement studies indicated that binding occurred to one class of noninteracting homogeneous receptors. The binding capacity for intact and lysed cells and membranes was 20 (±2) × 10−-11M for DPE2 and 43 (±2) × 10−-11M for DALEA. The enkephalin monomers [d-Ala2, d-Leu5]enkephalin (DADLE) and [d-Ala2, Met5]enkephalin amide (DAMEA) showed binding characteristics similar to those of DALEA.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1471-4159.1985.tb05480.x

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3

Digitale Medien

Is the benzodiazepine receptor coupled to a chloride anion channel? (reply) (1979)

RODBARD, DAVID ; COSTA, TOMMASO ; PERT, CANDACE B.

[s.l.] : Nature Publishing Group

Nature 280 (1979), S. 173-174

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ISSN: 1476-4687

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Notizen: [Auszug] In our recent study1, only five anions (G, Br, Cl, NO^ and SCN") of a series of 18 tested increased the affinity of diazepam binding to rat brain membranes. Confirming the report of Tallman et al.2, Martin and Candy3 have reported the enhancement of diazepam binding by ?-aminobutyric acid (GABA) ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/280173a0

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4

Digitale Medien

Is the benzodiazepine receptor coupled to a chloride anion channel? (1979)

COSTA, TOMMASO ; RODBARD, DAVID ; PERT, CANDACE B.

[s.l.] : Nature Publishing Group

Nature 277 (1979), S. 315-317

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ISSN: 1476-4687

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Notizen: [Auszug] Fig. I Effect of various anions on 3H-diazepam binding. Male rats (200-250 g) were decapitated. Cerebral cortices were washed quickly in ice-cold Tris-maleate buffer (50 mM, pH 7.6 at 0 ?C), homogenised in 100 vol of the same buffer (w/v) using a Brink-mann Polytron for 20 s and centrifugea at ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/277315a0

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5

Digitale Medien

Dimeric tetrapeptide enkephalins display extraordinary selectivity for the δ opiate receptor (1982)

Shimohigashi, Yasuyuki ; Costa, Tommaso ; Chen, Hao-Chia ; [weitere]

[s.l.] : Nature Publishing Group

Nature 297 (1982), S. 333-335

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ISSN: 1476-4687

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Notizen: [Auszug] A series of dimeric tetrapeptide enkephalins have been synthesized by a two-step coupling procedure: cross-linking of Boc-Phe-OH with NH2-(CH2)n-NH2 (where w=2-12), followed by elongation with Boc-Tyr-D-Ala-Gly-OH. Purity was confirmed by mass spectrometry, amino acid analysis and TLC. [D-Ala2, ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/297333a0

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6

Digitale Medien

Binding characteristics of a series of dimeric tripeptide enkephalins for δ opiate receptors in rat brain and NG108-15 cells (1989)

Shimohigashi, Yasuyuki ; Kodama, Hiroaki ; Costa, Tommaso ; [weitere]

Chicester [u.a.] : Wiley-Blackwell

Journal of Molecular Recognition 2 (1989), S. 127-131

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ISSN: 0952-3499

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Medizin

Notizen: The N-terminal tripeptide enkephalin analogue, Tyr-D-Ala-Gly, was dimerized at the C-terminus systematically with a series of α,ω-diaminoalkanes, NH2—(CH2)n—NH2 (n = 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, and 22). The binding affinities of dimers for δ opiate receptors in rat brain were evaluated and compared with those for δ receptors in NG108-15 cells. Although the monomeric tripeptide amide was almost inactive, dimers showed a dramatic increase in binding affinity (8-900 times). The enhancement of affinity was apparently related to the number of methylene chains in the crosslinking spacer moiety, and it was maximal at n = 14-18 in the rat brain. In NG cells the activity increased progressively from n = 2 to n = 22 without reaching any apparent peak. These results suggest that δ receptors in rat brain and NG cells may have slight structural differences.

Zusätzliches Material: 1 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jmr.300020305

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