In:
Journal of the American Society for Horticultural Science, American Society for Horticultural Science, Vol. 115, No. 4 ( 1990-07), p. 691-696
Abstract:
Two studies were conducted to test the effects of various tissue culture media on somatic embryogenesis from cotyledon tissue of cucumber (Cucumis sativus L.). The two best media for embryo initiation were Murashige and Skoog (MS) salts and vitamins containing either 1 or 2 mg 2,4-D/liter and 0.5 mg kinetin/liter. In the second study, embryos developed more normally. More plantlets developed when tissue was removed from the initiation medium after 3 weeks and transferred to MS containing 1 mg NAA/liter and 0.5 mg kinetin/liter for 3 weeks, rather than leaving the embryos on a medium containing 2,4-D. Histological evidence indicated that the embryos were multicellular in origin. Charcoal in the maturation medium inhibited embryo development. Chemical names used: (2,4-dichlorophenoxy) -acetic acid (2,4-D); N-(2-furanylmethyl)-lH-purine-6-amine (kinetin); 1-naphthaleneacetic acid (NAA).
Type of Medium:
Online Resource
ISSN:
0003-1062
,
2327-9788
DOI:
10.21273/JASHS.115.4.691
Language:
Unknown
Publisher:
American Society for Horticultural Science
Publication Date:
1990
detail.hit.zdb_id:
2040057-3
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