GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Comparative Studies. Non-European Languages/Literatures  (4)
Material
Publisher
Person/Organisation
Language
Years
FID
Subjects(RVK)
  • Comparative Studies. Non-European Languages/Literatures  (4)
RVK
  • 1
    Online Resource
    Online Resource
    Cytotoxicity enhancement of DOX-liposome-containing microbubbles combined with ultrasound to the multi-drug resistant cell
    Acoustical Society of America (ASA) ; 2012
    In:  The Journal of the Acoustical Society of America Vol. 131, No. 4_Supplement ( 2012-04-01), p. 3366-3366
    Details
    In: The Journal of the Acoustical Society of America, Acoustical Society of America (ASA), Vol. 131, No. 4_Supplement ( 2012-04-01), p. 3366-3366
    Abstract: Multidrug resistance (MDR) in cancer cells is a significant obstacle to successful cancer therapy. Doxorubicin (DOX) is very active chemotherapeutic agent for the treatment of breast cancer and the efficacy of DOX is also restricted by multidrug resistance.Ultrasound (US) targeted destruction of drug loaded microbubbles (MBs) is gaining more and more attention as a promising strategy for locally drug delivery. In this article, through avidin–biotin binding of DOX-containing liposomes to the microbubbles, we developed DOX-liposome-containing microbubbles in order to investigate its effect of enhancing cellular uptake and cytotoxicity of DOX against drug-resistant cancer cells. The results demonstrated that treatment of cells with ultrasound and DOX-liposome-containing microbubbles caused a significant higher drug uptake in DOX-resistant MCF-7 cells, compared with control (DOX-liposome). More importantly, a significant enhancement of tumor growth inhibition against DOX-resistant MCF-7 cells was found when using the drug-liposome-containing microbubbles combined with ultrasound.Compared with DOX-liposome treatment, the cytotoxicity effect was greatly enhanced from 21% to 60%. By further mechanism study, DOX-loaded microbubbles plus ultrasound induced significant apoptosis in MDR line of MCF-7 cells.
    Type of Medium: Online Resource
    ISSN: 0001-4966 , 1520-8524
    URL: Article
    DOI: 10.1121/1.4708690
    RVK:
    EQ 1000
    Language: English
    Publisher: Acoustical Society of America (ASA)
    Publication Date: 2012
    detail.hit.zdb_id: 1461063-2
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    A novel formulation of lipid microbubbles coated with stearic acid modified polyethylenimine to enhance ultrasound-mediated gene delivery in vitro
    Acoustical Society of America (ASA) ; 2012
    In:  The Journal of the Acoustical Society of America Vol. 131, No. 4_Supplement ( 2012-04-01), p. 3366-3366
    Details
    In: The Journal of the Acoustical Society of America, Acoustical Society of America (ASA), Vol. 131, No. 4_Supplement ( 2012-04-01), p. 3366-3366
    Abstract: The aim of this study was to assess the properties of a new designed cationic microbubbles as gene carriers and the relative gene transfection efficacy with ultrasound triggered microbubble destruction. Polyethylenimine as a high efficient gene transfection agent has higher cell toxicity with molecular weight increasing. Stearic acid was used to modify branched polyethylenimine to change its hydrophilic properties so that it can be assembled onto the lipid shell of the microbubbles and simultaneously decrease its cell toxicity. Cationic microbubbles was prepared by encapsulating perfluoropropane into phospholipids and stearic acid modified polyethylenimine hybrid shell using mechanical vibration method. The mean, median size and zeta potential of the microbubbles were measured 1.84±1.62um, 1.60um and 54mv respectively. Hoechst 33258 was used to stain the green fluorescent protein reporter plasmid which was charge-coupled to cationic microbubbles, and microbubbles was observed emitting blue light under a fluorescence microscope. About 4ug plasmid loaded by 108 microbubles that contain 5% mole ratio stearic acid modified polyethylenimine was measured by gel electrophoresis. A 1.25MHz single element transducer was used to mediate the gene transfection to MCF-7 cell by using the cationic microbubbles and enhancement of green fluorescent protein expression was observed.
    Type of Medium: Online Resource
    ISSN: 0001-4966 , 1520-8524
    URL: Article
    DOI: 10.1121/1.4708688
    RVK:
    EQ 1000
    Language: English
    Publisher: Acoustical Society of America (ASA)
    Publication Date: 2012
    detail.hit.zdb_id: 1461063-2
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Paclitaxel-liposome loaded microbubbles for ultrasound-triggered drug delivery in vitro and in vivo
    Acoustical Society of America (ASA) ; 2012
    In:  The Journal of the Acoustical Society of America Vol. 131, No. 4_Supplement ( 2012-04-01), p. 3366-3366
    Details
    In: The Journal of the Acoustical Society of America, Acoustical Society of America (ASA), Vol. 131, No. 4_Supplement ( 2012-04-01), p. 3366-3366
    Abstract: The aim of this work was to develop paclitaxel-liposome loaded microbubbles (PTX-loaded MBs) and to investigate the efficacy of chemotherapy through ultrasound-triggered drug delivery in vitro and in vivo. PTX-loaded liposomes were prepared by a minor modification of thin film hydration method and further conjugated to the surface of microbubbles through biotin-avidin linkage. The resulting payload-loaded MBs were characterized and applied to ultrasound assisted chemotherapy in breast cancer. Our results showed the MBs were able to achieve satisfactory drug encapsulation efficiency. Under ultrasound exposure, about 9.54-fold higher drug release and a significant improvement of cell uptake than that of loaded liposomes were observed. In addition, PTX-loaded MBs showed significantly greater tumor growth inhibition both in vitro and in vivo xenograft growth of breast tumor cells, compared with PTX-loaded liposomes and unloaded MBs. Drug distributions assay in various organs (heart, liver, spleen, lung, kidney and tumor) indicated about 3-fold higher PTX enriched in tumor. Histological examinations further demonstrated the tumor growth inhibition might contribute to increased apoptosis and reduced angiogenesis in tumor xenografts. In conclusion, the study indicated the PTX-loaded MBs significantly increased the anti-tumor efficacy and can be used as a potential chemotherapy approach for ultrasound assisted breast cancer treatment.
    Type of Medium: Online Resource
    ISSN: 0001-4966 , 1520-8524
    URL: Article
    DOI: 10.1121/1.4708689
    RVK:
    EQ 1000
    Language: English
    Publisher: Acoustical Society of America (ASA)
    Publication Date: 2012
    detail.hit.zdb_id: 1461063-2
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Dependence of the subharmonic signal from ultrasound contrast microbubbles on ambient pressure
    Acoustical Society of America (ASA) ; 2012
    In:  The Journal of the Acoustical Society of America Vol. 131, No. 4_Supplement ( 2012-04-01), p. 3324-3324
    Details
    In: The Journal of the Acoustical Society of America, Acoustical Society of America (ASA), Vol. 131, No. 4_Supplement ( 2012-04-01), p. 3324-3324
    Abstract: Ultrasound contrast agents (UCA) micro-bubbles have been well recognized as a potential noninvasive tool for blood pressure estimation. However, previous UCA indices, e.g., the shift of the resonance frequency, echo amplitude and the disappearance time, suffered from problems of low resolution, nonlinearity in the relationship with blood pressure, and only variations of the local pressure but the absolute values etc. In this paper, the effect of ambient pressure on UCA sub-harmonic optimal driving frequency (SODF) was investigated, at which the sub-harmonic scattering signals were the maximum. By applying transmit frequencies between 3MHz and 8MHz and the acoustic pressure of 300kPa, the acoustic attenuation and scattering of micro-bubbles were measured at overpressures of 4mmHg, 50mmHg and 100mmHg, comparable with the healthy human blood pressure. For groups of micro-bubbles with 80% in the diameter range of 1-2 micrometers, the shift of the SODF (SSODF) was 0.6MHz between over-pressures of 4mmHg and 100mmHg, which was approximately twice of the corresponding shift of the resonance frequency, thus had an improved sensitivity of pressure estimation. The SSODF of UCA micro-bubbles may be as a novel and sensitive index of the local blood pressure estimation.
    Type of Medium: Online Resource
    ISSN: 0001-4966 , 1520-8524
    URL: Article
    DOI: 10.1121/1.4708438
    RVK:
    EQ 1000
    Language: English
    Publisher: Acoustical Society of America (ASA)
    Publication Date: 2012
    detail.hit.zdb_id: 1461063-2
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...