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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 32 (1985), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The cytosol fraction of a symbiont-bearing strain of Amoeba proteus exerted a lethal effect when injected into symbiont-free amoebae of the original strain. The lethal factor appeared to be a protein with a molecular weight of over 200,000. While the effect of the lethal factor on the nucleus was reversible, the host cytoplasm was permanently damaged so that it could not form a viable cell when combined with a normal nucleus.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 32 (1985), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Long neglected has been the extensive and more or less intimate association of protozoa with a wide variety of other cells, either prokaryotic or eukaryotic in nature. Yet study of such relationships can provide important information concerning certain basic aspects of cellular evolution in general. A survey is offered here of the whole range of such symbiotic associations (i.e. with species of protozoa serving as hosts) with the purposes of drawing attention to the exciting possibilities of such research and of reviewing significant findings made to date. Because of the vastness of the overall field, examples and discussion are primarily limited to consideration of the following major studies: methanogenic bacteria in certain ciliates, bacterial endosymbionts of the large freshwater amoeba Pelomyxa palustris (itself an amazing organism from an evolutionary/phylogenetic point of view), the rod-shaped bacteria found in Amoeba proteus, the “Greek-letter” prokaryotes of Paramecium species, the xenosomes (sensu stricto) of the marine scuticociliate Parauronema acutum, and the diverse algal endosymbionts of similarly diverse protozoan taxa–ciliates, flagellates, radiolarians, acantharians, and foraminifera.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cells of Amoeba proteus and Chaos carolinensis that were in the process of phagocytosing large prey organisms were studied to find a structural basis for the generation of mechanical forces exerted by newly forming food cups. It was found that the food-cup walls facing prey organisms have a more prominent network of thin filaments inside the plasmalemma and that the glycocalyx covering the area is more condensed than usual.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 23 (1976), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Phagocytosing Amoeba proteus at different stages of forming foodcups have been observed by scanning electron microscopy. A nonphagocytosing ameba is characterized by dorsal and lateral ridges running longitudinally over the posterior half of the cell and its attachment to the substrate over small areas. When stimulated by prey organisms, the ameba loses polarity and ridges, and adheres to the substrate more firmly over a wider area of contact. Then it forms broad pseudopods to surround its prey and this results in the formation of foodcups. The surface of all amebae is covered with small projections, and membranous blebs are often seen on the surface of phagocytosing organisms.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 34 (1987), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A monoclonal antibody was obtained against a 29-kD polypeptide in the cytosol of a symbiont-bearing strain (xD) of Amoeba proteus and was used to determine the distribution of the antigen in amoebae. The 29-kD polypeptides (xD protein) are produced by bacterial endosymbionts that are necessary for the survival of host xD amoebae. Results of indirect immunofluorescent and electron-microscopic immunogold-labeling studies showed that the xD protein was present diffusely in the amoeba cytoplasm as well as in the symbiotic bacteria. The native protein containing 29-kD polypeptides was purified using an immunoaffinity column prepared with the monoclonal antibody and its molecular weight was determined to be 87,000.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Two-dimensional gel electrophoresis was used to compare the protein composition of normal (D strain) and symbiont-containing (xD strain) A. proteus. Over 500 different peptide spots could be identified on a typical gel of cytosol proteins. In addition to differences in a few minor peptides, one prominent polypeptide with an isoelectric point of about 5.5 and a molecular weight of about 29,000 was present in the cytoplasm of symbiont-containing amoebae and in the endosymbionts but not in D-strain amoebae. When endosymbionts were removed from xD-strain amoebae, the xD-specific polypeptide also disappeared, suggesting that it is produced by the endosymbionts.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 24 (1977), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. The effect of chloramphenicol (CAP) on the bacterial endosymbiotes of a strain of Amoeba proteus was studied by growing the symbiotic amebae in media containing 0.5–1.6 mg/ml CAP for up to 4 weeks. Treatments with CAP caused such ultrastructural changes as expansion of the nuclear zone and deformation of symbiotes. The CAP treatment also damaged the mitochondria, e.g. disappearance of central and protrusion of peripheral cristae. Number of bacteria per ameba decreased to 〈 10% of control in CAP-containing media, but no viable amebae became completely free of symbiotes. The resuts supported previous studies that amebae were dependent on endosymbiotes.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 221 (1969), S. 1073-1074 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Amoeba discoides, cultured in Chalkley's medium with Tetrahymena as a food organism10, was starved for 24 h and exposed to AM in fresh medium at 18 C. The treated amoebae were washed and cultured individually. They were observed directly, or were fixed in osmium tetroxide, embedded in Maraglass ...
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  • 9
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 195 (1962), S. 400-401 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] It has recently been shown that the anterior portion of an advancing amoeba is depolarized, and this fact has been incorporated in a theory of amoeboid movement4. In an investigation, to be reported elsewhere5, it has been found that heparin and some other natural mucopolysaccharides also ...
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  • 10
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 198 (1963), S. 675-676 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A CENTRAL problem in cell biology is cellular movement and locomotio Many workers investigate the locomotion of amoebae and several mechanisms have been proposed to account for the phenomena observed1"3. We have come to the conclusion that a satisfactory account of amoeboid locomotion must ...
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