ISSN:
1573-5125
Keywords:
alkaline phosphatase activity
;
enzyme interaction
;
nutrient regeneration
;
aminopeptidase activity
;
substrate stimulation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract From the observed pattern of aminopeptidase and alkaline phosphatase activities in the Baltic Sea, the question arose whether there is an interaction between the activities of both enzymes. In experiments with 0.8 μm filtered seawater, the effects of commercial alkaline phosphatase on bacterial aminopeptidase, the effects of commercial peptidase on bacterial alkaline phosphatase activity (APA), and the effects of proteins, carbohydrates and inorganic nutrients on the activities of both enzymes were investigated. Addition of commercial alkaline phosphatase stimulated bacterial aminopeptidase activity and, similarly, the addition of commercial peptidase increased the APA in bacteria. The proteins, albumin and casein, stimulated aminopeptidase activity and APA simultaneously. Experiments using ammonium and glucose suggested that stimulation of APA by peptidase could be mediated by nitrogen and carbon availability. There were also some indications that stimulation of aminopeptidase activity by alkaline phosphatase functioned by catalysing phosphate release from organic phosphorus compounds.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1011482815250
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