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  • 1
    ISSN: 1435-5922
    Keywords: Key words: hepatitis C virus ; nonstructural gene 5 ; quasispecies ; sequence analysis ; interferon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: The hepatitis C virus (HCV) quasispecies nature in the hypervariable region (HVR) has been reported and found to relate to the effectiveness of interferon (IFN) treatment. However, the quasispecies nature in the nonstructural (NS) 5b region remains to be addressed. To examine this characterization and relationship with IFN therapy, we sequenced six independent HCV clones from each of eight patients. The eight patients were classified as responders or nonresponders to IFN. In the four responders, we found one to three isolates in each of the six clones. In the nonresponders, the six clones consisted of four, five, six, and six isolates, respectively. Compared the (NS) 5b genes of the isolates obtained from the patients with that of the reported hepatitis C virus HC-C2 or HC-J6 isolate the ratio of nonsynonymous to total substitutions ranged from 17.61% to 30.95% in the responders and from 33.11% to 76.47% in the nonresponders. We also compared posttreatment with pretreatment sequences. The average number of varying amino acids ranged from 5.5 to 9.0 in isolates remaining after IFN treatment and from 4.3 to 5.5 in the isolates that disappeared with IFN treatment. Two changed amino acids (glycine to arginine and valine to isoleucine) (compared with the pretreatment clones) were found in the posttreatment clones of one of the responders and one amino acid change (valine to alanine) was found in another responder. These results suggest that the NS5b quasispecies correlates with IFN treatment effectiveness. These results also implied that the heterogeneity in different hierarchical strata has a common impact on IFN treatment, making infected patients resistant to IFN. Our study also provides evidence that HCV elimination and mutation may occur simultaneously during IFN therapy.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-5922
    Keywords: Key words: hepatitis C virus ; nonstructural region 5 ; sequence analysis ; China
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: Sequence variation in the putative nonstructural region 5b (NS5b) of hepatitis C virus (HCV) was analyzed in China. Complementary DNA fragments from sera of 49 Chinese patients were amplified by polymerase chain reaction (PCR) and the products were cloned and sequenced. Based on the comparison in NS5b of 33 clones of genotype 1b and 16 clones of genotype 2a, Chinese isolates of HCV belong to the same subtype as HCV-J, and HC-J6 from Japan. There does exist, however, some heterogeneity in the primary structure of the nucleotide acid. Higher homology was found among Chinese isolates than among Chinese isolates and Japanese isolates. Furthermore, among Chinese isolates, we found some conserved nucleotide acid positions different from those of Japanese isolates. Comparison of average homology among the 33 clones of genotype 1b and the 16 clones of genotype 2a indicated that the average homology among genotype 2a was lower than that among genotype 1b. In addition, a deletion of three nucleotide acids and a frame-shift, resulting in the introduction of an in-frame stop codon, were first observed in the NS5b region. These results indicated geographical differences in the distribution of individual HCV isolates, and the existence of a local variant in the same subtype. Our findings also suggested the need for further study on the sequence of genotype 2a, to improve diagnosis and help to advance the development of a vaccine.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2021-05-19
    Description: Taiwan Island is at the joint of Eurasian Continent and Pacific Plate, under threatening of typhoons and northeasterly strong winds. Consequently, enormous human lives and properties are lost every year. It is necessary to develop a coastal sea-state monitoring system. This paper introduces the coastal sea-state monitoring system (CSMS) along Taiwan coast. The COMC (Coastal Ocean Monitoring Center in National Cheng Kung University) built the Taiwan coastal sea-state monitoring system, which is modern and self-sufficient, consisting of data buoy, pile station, tide station, coastal weather station, and radar monitoring station. To assure the data quality, Data Quality Check Procedure (DQCP) and Standard Operation Procedure (SOP) were developed by the COMC. In further data analysis and data implementation of the observation, this paper also introduces some new methods that make the data with much more promising uses. These methods include empirical mode decomposition (EMD) used for the analysis of storm surge water level, wavelet transform used for the analysis of wave characteristics from nearshore X-band radar images, and data assimilation technique applied in wave nowcast operation. The coastal sea-state monitoring system has a great potential in providing ocean information to serve the society.
    Description: Published
    Keywords: Mooring buoys ; Acoustic waves ; Monitoring
    Repository Name: AquaDocs
    Type: Journal Contribution , Non-Refereed , Article
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