ISSN:
1432-0983
Schlagwort(e):
Key words Mismatch repair
;
mutS and mutL homologs
;
Exonucleases
;
Intragenic recombination
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract Besides the MutLS-like system, Schizosaccharomyces pombe has an additional pathway of mismatch repair. This minor pathway, producing short excision tracts, repairs C/C and, with lower efficiency, other mismatches also. We investigated the involvement of the exo1 +, msh2 + and pms1 + genes in the two pathways. The exo1 + gene encodes a 5′ to 3′ exonuclease, while msh2 + and pms1 + are homologs of Escherichia coli mutS and mutL, respectively. Intragenic two-factor crosses showed that exo1 +, msh2 + and pms1 + are involved in the major, but not in the C/C-correcting, pathway. Post-meiotic segregation frequencies and mitotic mutation rates in single and double mutants supported this finding. Furthermore, msh2Δ was epistatic over exo1Δ, and the ExoI enzyme is likely to be redundant with other exonucleases.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s002940050405
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