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  • 1
    Online Resource
    Online Resource
    Tropical Forests : Regional Paths of Destruction and Regeneration in the Late Twentieth Century. (2005)
    New York :Columbia University Press,
    Details
    Keywords: Deforestation-Tropics. ; Rain forest conservation. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (250 pages)
    Edition: 1st ed.
    ISBN: 9780231506908
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=5275837
    DDC: 333.751370913
    Language: English
    Note: Intro -- Table of Contents -- List of Figures -- List of Tables -- Preface -- Acknowledgments -- 1. Introduction -- 2. Theory and Method in Studying Regional Deforestation Processes -- 3. Central America and the Caribbean: Island and Isthmus Deforestation -- 4. The Amazon Basin: The Breakdown of Passive Protection -- 5. West Africa: From Cocoa Groves in Forests to Food Crops in Scrub Growth -- 6. Central Africa: Passive Protections for Rain Forests -- 7. East Africa: Sustainable Spots Surrounded by Degrading Expanses -- 8. South Asia: A Turning Point for Forests? -- 9. Southeast Asia: Deforesting the Lowlands, Afforesting the Highlands -- 10. Through a Regional Lens: Conservation Policies in Large and Small Forests -- Appendix: Case Studies and Accompanying QCAs for Each Region -- Notes -- References -- Index.
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  • 2
    Electronic Resource
    Electronic Resource
    Construction of hermes shuttle vectors: a versatile system useful for genetic complementation of transformable and non-transformableNeisseria mutants (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 558-569 
    Details
    ISSN: 1617-4623
    Keywords: Plasmid vector ; Conjugation ; Generalized mutagenesis ; Homologous recombination ; Natural transformation competence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A versatile shuttle system has been developed for genetic complementation with cloned genes of transformable and non-transformableNeisseria mutants. By random insertion of a selectable marker into the conjugativeNeisseria plasmidptetM25.2, a site within this plasmid was identified that is compatible with plasmid replication and with conjugative transfer of plasmid. Regions flanking the permissive insertion site of ptetM25.2 were cloned inEscherichia coli and served as a basis for the construction of the Hermes vectors. Hermes vectors are composed of anE. coli replicon that does not support autonomous replication inNeisseria, e.g. ColE1, p15A, orori fd, fused with a shuttle consisting of a selectable marker and a multiple cloning site flanked by the integration region of ptetM25.2. Complementation of a non-transformableNeisseria strain involves a three-step process: (i) insertion of the desired gene into a Hermes vector; (ii) transformation of Hermes into aNeisseria strain containing ptetM25.2 to create a hybrid ptetM25.2 via gene replacement by the Hermes shuttle cassette; and (iii) conjugative transfer of the hybrid ptetM25.2 into the finalNeisseria recipient. Several applications for the genetic manipulation of pathogenicNeisseriae are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174444
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Construction of Hermes shuttle vectors: a versatile system useful for genetic complementation of transformable and non-transformable Neisseria mutants (1996)
    Springer
    Molecular genetics and genomics 250 (1996), S. 558-569 
    Details
    ISSN: 1617-4623
    Keywords: Key words Plasmid vector ; Conjugation ; Generalized mutagenesis ; Homologous recombination ; Natural transformation competence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A versatile shuttle system has been developed for genetic complementation with cloned genes of transformable and non-transformable Neisseria mutants. By random insertion of a selectable marker into the conjugative Neisseria plasmid ptetM25.2, a site within this plasmid was identified that is compatible with plasmid replication and with conjugative transfer of plasmid. Regions flanking the permissive insertion site of ptetM25.2 were cloned in Escherichia coli and served as a basis for the construction of the Hermes vectors. Hermes vectors are composed of an E. coli replicon that does not support autonomous replication in Neisseria, e.g. ColE1, p15A, or ori fd, fused with a shuttle consisting of a selectable marker and a multiple cloning site flanked by the integration region of ptetM25.2. Complementation of a non-transformable Neisseria strain involves a three-step process: (i) insertion of the desired gene into a Hermes vector; (ii) transformation of Hermes into a Neisseria strain containing ptetM25.2 to create a hybrid ptetM25.2 via gene replacement by the Hermes shuttle cassette; and (iii) conjugative transfer of the hybrid ptetM25.2 into the final Neisseria recipient. Several applications for the genetic manipulation of pathogenic Neisseriae are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02174444
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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