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  • 1
    Publication Date: 2022-11-10
    Description: Dataset: Exudate Uptake Incubations - Microbial Abundances (Flow Cytometry)
    Description: Incubation experiments were conducted in St. John, US Virgin Islands to investigate the composition of exudates released from different species of benthic organisms, and the response of reef seawater microbial communities to mixed exudates released from different species and to specific metabolites. Exudates were collected from the stony coral Porites astreoides, and the octocoral Gorgonia ventalina after an 8 hour incubation. Reef seawater microbial communities were incubated separately in the presence of exudates from P. astreoides and G. ventalina for 48 hours and samples were collected to monitor changes in microbial abundance via flow cytometry and microbial community composition via 16S rRNA gene sequencing. Complementary Targeted and Untargeted metabolomic data from these incubation experiments is available on the MetaboLights database under accession number MTBLS2855. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/865739
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1736288
    Keywords: coral reef ; Planktonic Microorganisms ; Prochlorococcus ; Synechococcus ; Cell Counts ; flow cytometry ; Microbial Communities ; 16S rRNA gene sequencing
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
    Location Call Number Limitation Availability
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  • 2
    Publication Date: 2022-11-10
    Description: Dataset: Metabolite Uptake Incubations - Microbial Abundances (Flow Cytometry)
    Description: Pre-filtered reef seawater microbial communities collected from Lameshur Bay, U.S. Virgin Islands were incubated separately in the presence of the individual metabolites riboflavin, pantothenic acid, and caffeine for 24 hours and samples were collected to monitor changes in microbial community composition using 16S rRNA gene sequencing and microbial abundances using flow cytometry. Targeted metabolomic data from these incubations is available on the MetaboLights database under accession number MTBLS3286. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/865159
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1736288
    Keywords: coral reef ; Planktonic Microorganisms ; Prochlorococcus ; Synechococcus ; Cell Counts ; flow cytometry ; Microbial Communities ; 16S rRNA gene sequencing
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
    Location Call Number Limitation Availability
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