ISSN:
0006-3525
Schlagwort(e):
secondary structure analysis
;
copper,zinc superoxide dismutase and metal ion free derivatives
;
fourier transform ir spectroscopy
;
Chemistry
;
Polymer and Materials Science
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
Copper,zinc superoxide dismutase (Cu2Zn2-SOD) from bovine erythrocyte and its metal ion free derivatives, E2Zn2-SOD, Cu2E2-SOD, and E2E2-SOD (E: empty) were prepared and their secondary structures were investigated by Fourier transform ir spectroscopy. In 20 m M deuterated phosphate buffer (pD 7.5) solution at room temperature, the native Cu2Zn2-SOD contains about 34% β-strand, 17% β-turn, and 49% unordered structures, which is similar to the content determined by x-ray crystal structural analysis. The metal ion free derivatives decrease the component of β-strand and increase the unordered structure component in trend. Especially in the cases of zinc-free derivatives, Cu2E2-SOD and E2E2-SOD, about 24% β-strand, 20% β-turn, and 57% unordered structures are obtained. The result indicates that the zinc ion plays an important role in determining the secondary structure of copper,zinc superoxide dismutase. © 1997 John Wiley & Sons, Inc. Biopoly 42: 297-303, 1997
Zusätzliches Material:
3 Ill.
Materialart:
Digitale Medien
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