GLORIA — GEOMAR Library Ocean Research Information Access

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

The basic proteins of male gametic nuclei isolated from pollen grains of Lilium longiflorum (1994)

Ueda, Kenji ; Tanaka, Ichiro

Springer

Planta 192 (1994), S. 446-452

add to mindlist on the mindlist

Details

ISSN: 1432-2048

Keywords: Chromatin ; Generative nucleus ; Histone ; Lilium ; Nuclear differentiation ; Pollen

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A method has been developed for the efficient isolation of “generative” and “vegetative” nuclei from the generative and vegetative cells, respectively, of pollen grains of Lilium longiflorum Thunb. First, large numbers of pollen protoplasts were isolated enzymatically from nearly mature pollen grains. After the protoplasts had been gently disrupted by a mechanical method, the generative cells could be separated from the other pollen contents, which included vegetative nuclei. The generative nuclei were isolated by suspending the purified generative cells in a buffer that contained a non-ionic deter gent. The isolated generative nuclei, like those within pollen grains, had highly condensed chromatin and the isolated material was without contamination by vegetative nuclei. When basic proteins, extracted from the preparation of generative nuclei by treatment with 0.4 N H2SO4, were compared with those from preparations of somatic and vegetative nuclei by two-dimensional gel electrophoresis, it was revealed that at least five proteins with apparent molecular masses of 35, 33, 22.5, 21 and 18.5 kDa (p35, p33, p22.5, p21 and p18.5), respectively, were specific for, or highly concentrated in, the generative nuclei. An examination of solubility in 5% perchloric acid and the mobility during electrophoresis indicated that two of these proteins (p35 and p33) resembled H1 histones while the three other proteins (p22.5, p21 and p18.5) resembled core histones. It is likely that these basic nuclear proteins are related to the condensation of chromatin or to the differentiation of male gametes in flowering plants, as is the case for analogous proteins present during spermatogenesis in animals.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00198582

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Vinyl polymerization. 244Part 243. of the series: K. KUBUSHIRO, K. TAKEMOTO, and M. IMOTO, Makromolekulare Chem. 138 (1970) 1.. Polymerization of methyl and n-butyl methacrylate and n-butyl acrylate initiated by the system of cupra-rayon, water and carbon tetrachloride (1970)

Imoto, Minoru ; Ueda, Kenji ; Takemoto, Kiichi

New York : Wiley-Blackwell

Die Makromolekulare Chemie 138 (1970), S. 11-17

add to mindlist on the mindlist

Details

ISSN: 0025-116X

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Physics

Description / Table of Contents: Die Polymerisation von Methylmethacrylat wurde in Gegenwart von Cupra-rayon, Wasser und Tetrachlorkohlenstoff durchgeführt; sie verläuft radikalisch. Der Einfluß der zugesetzten Mengen an Monomerem, Cupra-rayon, Wasser oder Tetrachlorkohlenstoff auf die Polymerisationsgeschwindigkeit wurde untersucht, und aus den ermittelten Werten der Geschwindigkeiten wurde die Bruttoaktivierungsenergie im Temperaturbereich zwischen 70 und 90°C zu 9,7 kcal/Mol geschätzt. Weiter wurde gefunden, daß n-Butylacrylat sowie-methacrylat auch von dem oben erwähnten System polymerisiert werden, während Styrol und Acrylnitril sich nicht polymerisieren lassen.

Notes: Polymerization of methyl methacrylate was carried out in the presence of cupra-rayon, water and carbon tetrachloride. It was concluded that the process was of radical mechanism. The effects of the amount of methyl methacrylate, cupra-rayon, water or carbon tetrachloride on the rate of polymerization were investigated. From the rates of polymerization at 70-90°C, overall activation energy was estimated as 9.7 kcal/mole. n-Butyl acrylate and methacrylate were also polymerized by the above system. However, styrene and acrylonitrile did not polymerize.

Additional Material: 13 Ill.