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  • Ammonium; Benguela Upwelling System; BUSUC 1; Continuous Segmented Flow Analyzer, SEAL Analytical, QuAAtro39; CTD, Sea-Bird SBE 911plus; CTD/Rosette; CTD-RO; DATE/TIME; DEPTH, water; Event label; Field observation; LATITUDE; LONGITUDE; M157; M157_10-7; M157_11-4; M157_12-2; M157_14-2; M157_16-25; M157_16-3; M157_16-6; M157_17-16; M157_17-2; M157_24-1; M157_25-1; M157_2-8; M157_28-1; M157_2-9; M157_36-2; M157_41-14; M157_42-2; M157_43-2; M157_43-6; M157_9-2; Meteor (1986); Microstructure profiler, Sea & Sun Technology, MSS90L; Namibia; Nitrate; Nitrite; nutrients; Oxygen; oxygen minimum zone; PCTD-RO; Phosphate; PumpCTD/Rosette; Salinity; Sample code/label; Silicate; Station label; Temperature, water; Water mass; water mass fraction  (1)
  • Microinjection  (1)
Document type
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  • 1
    ISSN: 1432-2013
    Keywords: Diffusion coefficient ; Muscle cells ; Myoglobin ; Microinjection ; Oxygen ; Facilitated diffusion ; Intracellular oxygen transport ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We measured the diffusion coefficient of myoglobin (D Mb) inside mammalian skeletal muscle cells with a microinjection technique. A small bolus of horse Mb was injected into a single muscle fibre and the subsequent time-dependent changes of the Mb profiles along the fibre axis were measured with a microscope-photometer. For fibres of the rat soleus muscle at 22° C, a D Mb of 1.3·10−7 cm2/s was found, confirming a result obtained previously by us for rat diaphragm muscle with a photo-oxidation technique. In the extensor digitorum longus muscle of the rat, a higher value of 1.9 · 10−7 cm2/s was measured. Auxotonic muscle contractions did not change the apparent D Mb. For the temperature range between 22 ° C and 37 ° C, a temperature coefficient, Q 10, of 1.5 was calculated. The implication of this result for the role of Mb in the facilitation of oxygen transport was examined. Model calculations show that with this relatively low D Mb value, the intracellular oxygen supply can be improved only slightly.
    Type of Medium: Electronic Resource
    Location Call Number Limitation Availability
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  • 2
    Publication Date: 2024-03-22
    Description: Upwelling systems are significant sources of atmospheric nitrous oxide (N₂O). The Benguela Upwelling System is one of the most productive regions worldwide and a temporally variable source of N₂O. Strong O₂ depletions above the shelf are favoring periodically OMZ formations. We aimed to assess underlying N₂O production and consumption processes on different temporal and spatial scales during austral winter in the Benguela Upwelling System, when O₂-deficiency in the water column is relatively low. The fieldwork took place during the cruise M157 (August 4ᵗʰ – September 16ᵗʰ 2019) onboard the R/V METEOR. This expedition included four close-coastal regions around Walvis Bay at 23°S, which presented the lowest O₂ concentrations near the seafloor and thus may provide hotspots of N₂O production. Seawater was collected in 10 L free-flow bottles by using a rosette system equipped with conductivity-temperature-depth (CTD) sensors (SBE 911plus, Seabird-electronics, USA). Concentrations of inorganic nutrients (PO₄³⁻, NH₄⁺, NO₃⁻, NO₂⁻, and SiO₂) were measured colorimetrically according to Grasshoff et al. (1999) by means of a continuous segmented flow analyzer (SEAL Analytical, QuAAtro39). To determine the water mass fractions along the sampling transects, vertical profiles were collected using a free-falling microstructure profiler (MSS90L, Sea & Sun Technology). Temperature, dissolved oxygen, and salinity were measured with a CTD system consisting of a SeaBird 911+ probe, mounted on a sampling rosette.
    Keywords: Ammonium; Benguela Upwelling System; BUSUC 1; Continuous Segmented Flow Analyzer, SEAL Analytical, QuAAtro39; CTD, Sea-Bird SBE 911plus; CTD/Rosette; CTD-RO; DATE/TIME; DEPTH, water; Event label; Field observation; LATITUDE; LONGITUDE; M157; M157_10-7; M157_11-4; M157_12-2; M157_14-2; M157_16-25; M157_16-3; M157_16-6; M157_17-16; M157_17-2; M157_24-1; M157_25-1; M157_2-8; M157_28-1; M157_2-9; M157_36-2; M157_41-14; M157_42-2; M157_43-2; M157_43-6; M157_9-2; Meteor (1986); Microstructure profiler, Sea & Sun Technology, MSS90L; Namibia; Nitrate; Nitrite; nutrients; Oxygen; oxygen minimum zone; PCTD-RO; Phosphate; PumpCTD/Rosette; Salinity; Sample code/label; Silicate; Station label; Temperature, water; Water mass; water mass fraction
    Type: Dataset
    Format: text/tab-separated-values, 1660 data points
    Location Call Number Limitation Availability
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