Schlagwort(e):
Electronic books.
Materialart:
Online-Ressource
Seiten:
1 online resource (220 pages)
Ausgabe:
1st ed.
ISBN:
9783110879476
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=3042054
DDC:
579.342
Sprache:
Englisch
Anmerkung:
Intro -- Introduction -- Part 1: A Short History of the lac System from its Beginning to 1978 -- 1.1 From Noah to Pasteur: Adaptation in Yeast -- 1.2 Adaptation in Bacillus subtilis and Escherichia coli -- 1.3 Mutants in the lac System of Escherichia coli -- 1.4 Inducers of the lac System and the Discovery of Permease -- 1.5 Negative Control through Repressor -- 1.6 Further Evidence for Negative Control through Repressor -- 1.7 The Triumph of the Jacob-Monod Theory -- 1.8 Minor Defects in the Jacob-Monod Theory -- 1.9 Isolation of the Lac and λ CI Repressors -- 1.10 Putting the lac Genes on Phage φ80 or λ -- 1.11 Lac and λ CI Repressors Bind to their Operator DNAs -- 1.12 Making Grams of Lac Repressor to Determine its Sequence -- 1.13 Mutations in lacI Suggest a Modular Structure of Repressor -- 1.14 Miller's Analysis of the I Gene: Climbing Mount Everest -- 1.15 Positive Control through the CAP Protein -- 1.16 Isolation and Sequence of lac Operator -- 1.17 Chemical DNA Sequencing -- 1.18 Steric Hindrance is the Mechanism of Repression -- 1.19 Chemical Synthesis of lac Operator DNA -- 1.20 Lac Repressor-Operator Complex Binds to Nitrocellulose -- 1.21 Polyacrylamide Gel Electrophoresis of Protein-DNA Complexes -- 1.22 Fusion of and Complementation within the lacZ Gene -- 1.23 Isolation of Lac Permease -- 1.24 The End of an Era -- Part 2: Misinterpretation -- 2.1 The Interpretation of Experiments is Sometimes Wrong -- 2.2 Adaptation Explained by Self-Replicating Genes -- 2.3 The Kinetics of Adaptation -- 2.4 Lac Repressor is RNA -- 2.5 Operator is RNA or Protein -- 2.6 Isolation of Lac Repressor -- 2.7 Isolation of Lac Permease and Arg Repressor -- 2.8 Genetic Proof that lac Operator is Dyadic -- 2.9 The Core of Lac Repressor Binds to lac Operator -- 2.10 Lac Repressor Uses ß-Sheet to Recognize lac Operator.
,
2.11 A π-Helix Binds to a Hairpin Loop of lac Operator -- 2.12 Prediction of the Sequence of lac Operator -- 2.13 The Kinetics of lac Repressor-Operator Interaction -- 2.14 Lac Repressor Bound to O2 Acts as an Efficient Road Block -- 2.15 Auxiliary Operators Can be Disregarded in Repression -- 2.16 CAP Activates Transcription like λ CI Repressor -- 2.17 In the Pitfall of Symmetry -- 2.18 The CAP-DNA Complex: Two Propositions -- 2.19 The Adenine of Cyclic AMP Binds to DNA -- 2.20 The Structure of ß-Galactosidase -- 2.21 From Lamarck to Cairns -- 2.22 DNA Sequence Analysis: Not in this Century! -- 2.23 Protein Splicing -- 2.24 OI-Repressor-O2 Loops -- Part 3: The lac Operon, a Paradigm of Beauty and Efficiency -- 3.1 The New Perspective -- 3.2 Some Numbers and Concepts -- 3.3 Activation of the lac Promoter by CAP Protein -- 3.4 Repression of the lac Promoter by Lac Repressor -- 3.5 ß-Galactosidase, Lac Permease and Transacetylase -- 3.6 The lac System as a Tool -- 3.7 What Lesson can be Learned from the lac System? -- 3.8 Outlook -- Authors Index -- Subject Index.
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