ISSN:
1432-2048
Schlagwort(e):
Cell culture (cytokinin and cell division)
;
Cell division
;
Cytokinin and cell division
;
Glycine (cell culture)
;
Phosphatidylinositol
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract A method is described for preparing fully viable, cytokinin-starved soybean (Glycine max (L.) Merr. cv. Acme) cells from a suspension-culture of callus tissue. The cells respond to kinetin treatment by re-initiating cell division. We present evidence, from the pattern of incorporation of 32P-labelled inorganic phosphate into individual phospholipids during the first hour of this response, that the synthesis of phosphatidylinositol (PI) and of phosphatidic-acid head-groups is affected within 15 min. The polyphosphoinositide phosphatidylinositol 4-phosphate, but not phosphatidylinositol 4,5-bisphosphate, was detected in the tissue. The characteristics of cytokinin-induced PI synthesis in cytokinin-starved soybean cells appear to resemble the ‘PI response’ of animal cells.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00397884
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