ISSN:
1617-4623
Keywords:
Streptomyces
;
Antibiotics
;
Bialaphos
;
Resistance gene
;
Biosynthetic gene cluster
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have isolated and studied the organization ofStreptomyces hygroscopicus genes responsible for the biosynthesis of the antibiotic herbicide bialaphos. Bialaphos production genes were cloned from genomic DNA using a plasmid vector (pIJ702). Three plasmids were isolated which restored productivity toS. hygroscopicus mutants blocked at different steps of the biosynthetic pathway. Subcloning experiments using other nonproducing mutants showed that four additional bialaphos production genes were also contained on these plasmids. A gene conferring resistance to bialaphos, which was independently cloned using the plasmid vector pIJ61, and an antibiotic-sensitive host (S. lividans), was also linked to the production genes. Cosmids were isolated which defined the location of these genes in a 16 kb cluster.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02428031
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